An automated cell culture system includes a cell culture reactor including a housing; a fluidic circuit for cell culture media, the fluidic circuit disposed in an interior of the housing. The fluidic circuit includes a culture vessel for culturing cells in the cell culture media, a reservoir for the cell culture media, the reservoir fluidically connected to the culture vessel, and a pump configured to pump the cell culture media in the fluidic circuit. The automated cell culture system includes one or more sensors disposed in the interior of the housing, each sensor configured to detect a parameter of one or more of (1) the cell culture media in the fluidic circuit and (2) an environment in the interior of the housing; and a computing device configured to automatically control operation of the cell culture reactor based on one or more of the detected parameters.

Disclosed is an apparatus for the production of tissue from cells. The apparatus comprises an elongate body having at least one circumferential groove and being operable to extend, by close-fitting relationship, centrally through at least one trough. The troughs are extending in a closed path, such that the at least one of the circumferential grooves open into an inner edge of a trough.

Also disclosed is a process for production of tissue from cells, via a transitioning intermediate which transitions from the cells into the tissue.

A cell culturing system includes a cell culturing device and a support device. The cell culturing device cultures cells by allowing a culture medium inside circulation flow paths connected to the bioreactor to flow inside the bioreactor. The cell culturing device is connected to the circulation flow paths and includes a waste liquid flow path in order to discard the liquid flowing through the circulation flow paths, and a waste liquid accommodation unit in which the liquid guided from the waste liquid flow path is accommodated. A sampling unit, in which the culture medium that is guided from the circulation flow paths to the waste liquid flow path is collected, is connected to the waste liquid flow path.

Embodiments are described that relate to methods and systems for growing cells in a hollow fiber bioreactor. In embodiments, the cells may be exposed to a number of growth factors including a combination of recombinant growth factors. In other embodiments, the cells may be grown in co-culture with other cells, e.g., hMSC's. In embodiments, the cells may include CD34+ cells.

Described herein are biofilm bioreactors for synthesis at the interface between two liquids, and methods of using such bioreactors for the biotransformation of feedstocks into chemical products. Also contemplated is the extraction of such products.

In one aspect, the present invention pertains to a system for increasing the methane content of biogas (biogas upgrading). In another aspect, the invention relates to a method for increasing the methane content of biogas.

A membrane biofilm reactor including a vessel defining a volume is disclosed. The reactor also typically includes a first and second plurality of hollow fiber membranes. Each hollow fiber membrane generally has an outer surface and a lumen, and is located within the volume. The reactor further preferably includes a first and second gas feedstock. The first gas feedstock is provided through a first inlet in fluid communication with the lumens of the first plurality of hollow fiber membranes. The second gas feedstock is provided through a second inlet in fluid communication with the lumens of the second plurality hollow fiber membranes. Finally, the reactor also typically includes a biofilm formed on the outer surface of the hollow fiber membranes and made up of methanotrophs, Methanosarcina acetivorans, or both. The first gas feedstock is preferably different from the second gas feedstock.

A culture device and a culture method by which a substance to be cultured can be three-dimensionally cultured, and the substance thus cultured can be removed as an integrated product with multiple tubes; and a cultured organ produced by the culture method. The culture device comprises a sealed container (2) which contains the substance to be cultured (A) and is disassembled after the culture is completed; multiple ducts (3, 4, 5) arranged in the sealed container (2) and having micropore formed on the outer peripheral surface; a culture medium-feeding device (6, 7) for feeding/circulating the culture medium (B) to at least one duct (3 or 5); an excretory device (8) connected to at least one duct (4) for excreting the waste product (C) permeating into the duct (4) through the micropore of the duct (4) from the substance to be cultured to the outside of the sealed container.

Provided herein are tubular membrane filter elements, tangential flow filtration systems comprising such filter elements and methods of using such filter elements and filtration systems.

Provided herein is technology relating to engineered tissues and particularly, but not exclusively, to methods, compositions, and systems for engineering a biosynthetic vascular network.