A liquid cell culture medium collecting filter unit includes a porous metal membrane that filters out cells in a liquid cell culture medium, a support that holds a peripheral portion of the porous metal membrane. and a tubular member that has a hollow part serving a flow path for a liquid cell culture medium. The tubular member is connected to the support such that the flow path faces at least part of a main surface of the porous metal membrane.

Described herein are biofilm bioreactors for synthesis at the interface between two liquids, and methods of using such bioreactors for the biotransformation of feedstocks into chemical products. Also contemplated is the extraction of such products.

Provided herein are tubular membrane filter elements, tangential flow filtration systems comprising such filter elements and methods of using such filter elements and filtration systems.

There has been demand for an additional method for producing antibodies. The present invention provides: a polymer-coated crosslinked alginate gel fiber in which a core layer containing a crosslinked alginate gel and either antibody-producing cells (e.g., antibody-producing CHO cells) or bioactive-substance-producing cells (e.g., MIN6 cells derived from pancreatic ? cells) is coated with a cationic polymer; and a method for producing antibodies, a bioactive substance, etc., using the fiber.

An apparatus includes a sample holder to hold or receive a sample during use. The apparatus also includes a first chamber operably coupled to the sample holder and configured to receive the sample holder. The first chamber includes at least one extraction reagent for extracting nucleic acid from the sample during use to generate a first treated sample. The first chamber and the sample holder collectively define a common longitudinal axis. The apparatus also includes a second chamber operably coupled to the first chamber and disposed along the longitudinal axis. The second chamber includes at least one oligomer for amplifying the nucleic acid to generate a second treated sample.

Proposed are a three-dimensional structuring method of cells and a three-dimensional structuring system of cells capable of efficiently bonding multiple cell clusters in a three-dimensional direction, pursuant to their growth, while ensuring safety. A plurality of fibers, in which one end of each of the fibers is held by a flat plate, are inserted together with the flat plate into a flow path through which a culture solution is supplied, a plurality of cell clusters are placed in the flow path upon causing the cell clusters to run with a liquid flow of the culture solution, and each of the cell clusters is cultured by being stacked on an outer surface of each of the fibers with the flat plate as a growth origin.

The present disclosure provides an in vitro tubular blood brain barrier mimic used to model drug transport across the brain capillary endothelial barrier cells to neurons. In one embodiment the stack is comprised of interior neuro endothelial capillary cells, extracellular matrix, porous polymeric hollow fiber, exterior extracellular matrix, and neuron astrocytes. The tubular human blood brain barrier mimic is used to test molecular transport and effects of drug candidates across the multilayer stack.

A system and method adapted for continuous growth and harvesting of fungal fruiting bodies. The system includes a growth chamber, one or more mycelium feed assemblies, a nutrient reservoir with liquid media, one or more environmental controls to control an environment within the growth chamber and of the liquid media within the nutrient reservoir. One or more mycelium feed assemblies are arranged within the growth chamber. Each of the one or more mycelium feed assemblies includes a nutrient supply member and a mycelium colony which grows around the nutrient supply member, that continuously supplies liquid media to the mycelium colony. The method includes starting one or more mycelium colonies on the nutrient supply member, establishing that a mature colony has formed, and then maintaining the mature mycelium colony by continuous delivery of liquid medium to the mycelium colony, thus allowing for contiguous generation and harvesting of fungal fruiting bodies.

According to one embodiment, a cell culture apparatus includes a culture container, a motor, an ejecting pipe, and a discharging pipe. The culture container has a transverse hollow tubular shape, has an opening formed in the center of a bottom surface, and has an openable and closable lid portion formed on a side surface. The motor rotates the culture container around a rotation axis perpendicular to the bottom surface. The ejecting pipe is inserted from the opening into the culture container. The discharging pipe is connected to the lid portion. An inner wall surface of the culture container has a shape of a single spiral when viewed from the direction of the rotation axis. The ejecting pipe ejects a liquid onto the inner wall surface. The discharging pipe discharges the ejected liquid from the lid portion.

Tissue-processing containers are disclosed for facilitating multistep processing of tissue. Also disclosed are systems incorporating the tissue-processing containers that include shakers, incubators, controllers, and pumps. Multistep methods are disclosed for processing tissues using the tissue-processing containers. The tissue-processing containers are specially designed to achieve efficiencies in automated tissue processing, exposure of tissues to processing media, and multistep tissue processing protocols. The described tissue-processing containers comprise a cup-like cavity comprising a middle hole covered by a mesh screen, troughs for separate processing steps, and are stackable in a substantially airtight and substantially watertight manner. Tissues for processing using the described tissue-processing containers include nerve tissue. Also described herein is a transport housing for the transport of stacks of tissue-processing containers, and which may directly incubate those containers, or which may be placed inside a separate incubator for incubating the containers.