The disclosure relates to a method for generating cells which secrete neurotrophic factors (NTFs) in a functionally closed and automated hollow-fiber bioreactor system comprising inducing differentiation of a population of undifferentiated mesenchymal stem cells (MSCs) in a differentiating medium supplemented with ascorbic acid. The disclosure further relates to a method of treating a disease for which administration of neurotrophic factor is beneficial and to a pharmaceutical composition including the described cells.

The present disclosure relates to cell cultured adipose tissue. In one embodiment, the cultured adipose tissue is produced by culturing adipose cells in a culture media in vitro, harvesting the adipose cells after a desired amount of adipose cells are produced, and aggregating the harvested adipose cells to provide the cultured adipose tissue. In some embodiments, aggregating the harvested adipose cells comprises mixing the harvested adipose cells with a hydrogel or binder in a three-dimensional (3D) mold. In other embodiments, aggregating the harvested adipose cells comprises cross-linking the harvested adipose cells in a 3D mold. The cultured adipose tissue have a defined 3D shape and a size on the macroscale. In some embodiments, the cultured adipose tissue may be a food product.

Provided are a cell culture supplement for producing stem cells and a method for producing stem cells. The cell culture supplement is formed of an aqueous solution of a polypeptide represented by the following formula (1): (Pro-Hyp-Gly)n (1) wherein Hyp represents hydroxyproline, and n represents an integer of 2 to 1000.

A cell support composite includes: a substrate formed of an artificial material; a laminin molecule or a fragment thereof which adheres to at least a part of the substrate; and a renal tubule epithelial cell or a renal tubule epithelial-like cell which is a culture cell attached to the substrate via the laminin molecule or the fragment thereof.

A platform for creating engineered tissues includes a vascular tube that defines a vascular diameter and is configured to receive vascular system seed cells, a non vascular tube that defines a non-vascular tube diameter and is configured to receive organ system seed cells, and a barrier formed between the vascular tube and the non vascular tube.

A cell culture carrier module and a cell culture system having the same are provided. A cell tank and a culture medium module respectively communicate with the carrier module. The carrier module includes a reactor, a first fixer, a second fixer and a plurality of cell culture carriers. The reactor has a chamber and at least one inlet/outlet. The inlet/outlet communicates with the chamber. The first fixer is fixed to the reactor and located in the chamber. The second fixer is disposed in the chamber and is movable relative to the first fixer. Two ends of each cell culture carrier are fixed to the first fixer and the second fixer, respectively. The cell culture carriers are in an untwisted state or a twisted state according to a variation in a distance between the first fixer and the second fixer due to a movement of the second fixer.

A platform for creating engineered tissues includes a vascular tube that defines a vascular diameter and is configured to receive vascular system seed cells, a non-vascular tube that defines a non-vascular tube diameter and is configured to receive organ system seed cells, and a barrier formed between the vascular tube and the non-vascular tube.

A method of manufacture of crosslinked, edible, porous hollow fibers and sheet membranes suitable for the manufacture of clean meat products, the hollow fibers and sheet membranes made therefrom and methods of use thereof.

The invention relates to bioengineering and medical modular systems for the realization of self-contained equipment for influencing biological media, cells, tissue and tissue-like structures as objects. These modular systems distinguish themselves, in particular, by the fact that the user-specific equipment for influencing the objects can be realized in a simple and economically effective way. A tub-shaped main body for placement of elements that can be combined with one another is provided for this. Furthermore, at least one container for a liquid medium, a pumping system for a liquid medium, connection elements, filters for the inlet and outlet of gas, at least one filter with a connecting element for a medium, elements for a connection to at least one bioreactor, at least one gas inlet unit or a combination of at least one bioreactor and at least one gas inlet unit are components of the modular system. Moreover, an area for at least one bioreactor, at least one gas inlet unit or a combination with at least one bioreactor and at least one gas inlet unit is available. Straight pipe sections, bent pipe sections, hose sections, connectors and/or distributors can ideally be connection elements here.

Provided is a simple method for producing a cell concentrate (other than human blood) in a short time with neither loss of cells nor an excessive burden on cells. Included is a method for producing a cell concentrate using an inside-out filtration system for processing a cell suspension, the system including: a cell suspension inlet port; a filtrate outlet port; a cell suspension outlet port; and hollow fiber separation membranes provided between the cell suspension inlet port and the cell suspension outlet port, wherein the membranes have a total cross-sectional area of 0.5-1.5 cm.sup.2, the membranes have an inside membrane area of 0.2 m.sup.2 or less, the suspension is flowed inside the membranes at a linear velocity of 500-1200 cm/min, and the quotient of the division of the initial filtrate flow rate from the filtrate outlet port by the flow rate into the cell suspension inlet port is 0.4-0.7.