An insect culture maintenance system includes a sequence of open-ended cylindrical tubes [500, 502, 504, 506] joined pairwise alternately at their tops and bottoms using multiple dual-cap connectors. Each dual-capped connector has a channel from an inside of a first cap to an inside of a second cap. In use, connectors that cap the bottoms of the tubes [508, 512] are filled with insect food media [518, 520], while connectors that cap the tops of the tubes [510] are open. As a result of this design, adults pass from one tube to the next through the top dual-cap connectors, while larvae pass from one tube to the next through the bottom dual-cap connectors, resulting in propagation of subsequent generations of insects through the sequence of tubes.

A nozzle system for fluid deployment for treating a biological fluid within a bioreactor, having a bioreactor having an internal volume; an adjustable nozzle deployed within the internal volume; a reservoir capable of containing an agent; and a tubing connecting the reservoir and the adjustable nozzle, wherein the adjustable nozzle is capable of being adjusted to distribute a processing agent in a plurality of distribution streams.

A component management apparatus for a bioprocessing system includes a frame having a plurality of segments, including at least a first segment and a second segment pivotably connected to the first segment such that at least the second segment is movable between a closed position and an open position, and at least one mounting bracket connected to the frame for connection of a bioprocess component.

The devices, methods, and compositions disclosed herein accomplish robust cell encapsulation in polymer microparticles using a vertically oriented microfluidic device. A hydrophilic polymer precursor solution is flowed into a first inlet channel, which extends inward from an upper surface of the device housing. A hydrophobic fluid is flowed into a second inlet channel, which extends inward from a lower surface of the device housing. The two inlet channels meet at a junction, and an outlet channel extends away from the two inlet channels. When the two inwardly flowing streams meet at the junction, the polymer precursor solution disperses into the hydrophobic fluid. The dispersed precursor droplets are photopolymerized into microparticles as they travel through the outlet channel. The resulting microparticles are highly uniform, and are larger than conventionally formed microparticles. Cells of varying types can be encapsulated with high viability and spatial uniformity.

A culture device and a culture method by which a substance to be cultured can be three-dimensionally cultured, and the substance thus cultured can be removed as an integrated product with multiple tubes; and a cultured organ produced by the culture method. The culture device comprises a sealed container (2) which contains the substance to be cultured (A) and is disassembled after the culture is completed; multiple ducts (3, 4, 5) arranged in the sealed container (2) and having a plurality of micropores formed on the outer peripheral surface; a culture medium-feeding device (6, 7) for feeding/circulating the culture medium (B) to at least one duct (3 or 5); an excretory device (8) connected to at least one duct (4) for excreting the waste product (C)permeating into the duct (4) through the micropores of the duct (4) from the substance to be cultured to the outside of the sealed container.

The present invention is for a closed environment process for the growth and differentiation of cells and the culturing of cells to confluency for the production of tissue. The tissue may be a clean meat product.

An object of the invention is to make it possible to provide a mechanism that applies an external stimulus and to measure structural and electromagnetic changes of a cell due to the external stimulus with high sensitivity by an NV center. There is provided an environment control mechanism configured to change a state of a sample by applying an external stimulus to the sample.

The invention relates to a bioreactor (100) which is designed for cultivating biological cells (1), comprising a container (10) configured to receive a cultivation medium (2), and a plurality of substrate filaments (20) which are arranged in the container (10) and are configured for a temporary adherent coupling of the biological cells (1) to the substrate filaments (20). The substrate filaments (20) are provided with a surface layer (21) which is switchable between an adherence state, in which the biological cells (1) can be QI coupled adherently to the surface layer (21), and a release state, in which the adherent coupling of the biological cells (1) to the surface layer (21) is reduced in comparison to the binding state. The invention also relates to a method for processing biological cells (1) in the bioreactor (100).

A liquid cell culture medium collecting filter unit includes a porous metal membrane that filters out cells in a liquid cell culture medium, a support that holds a peripheral portion of the porous metal membrane. and a tubular member that has a hollow part serving a flow path for a liquid cell culture medium. The tubular member is connected to the support such that the flow path faces at least part of a main surface of the porous metal membrane.

The present invention is to provide a method capable of visualizing a coating state of a protein adsorbent even on a substrate having a complicated structure, in particular, for use on a surface of an apparatus for culturing or growing living cells. The method comprises coating a series of substrates with selected protein adsorbents and fluorescent dye, irradiating selected coated substrates and determining a coating state from color development of the substrates.