The present invention relates to an edible and sterilizable macroporous three-dimensional (3D) tissue engineering scaffolds comprising a network of cross-linked biocompatible polymer, preferably a natural polymer. Moreover, the scaffold of the invention further comprises additives and living cells which adhere and proliferate, colonizing the entire surface of the scaffold and giving rise to a raw material for the later formation of tissue with high nutritive content and/or cultured meat, that may be subsequently processed into food for animal or human consumption without requiring modification or removal of the cells form the scaffold. Method of using the scaffolds to make cultured meat and/or tissues for being processed as food comprising the scaffold, are also described herein.

The present invention relates to a process for producing a composition comprising an aqueous medium and, disposed in the aqueous medium, a first volume of a first hydrogel, which process comprises: (i) providing a composition comprising a first hydrophobic medium and, disposed in the first hydrophobic medium, a first volume of a first hydrogel; (ii) disposing a volume of an aqueous composition comprising a hydrogel compound around the first volume of the first hydrogel; (iii) allowing the aqueous composition comprising the hydrogel compound to form a gel and thereby forming a hydrogel object, which hydrogel object comprises the first volume of the first hydrogel and a second volume of a second hydrogel, which second volume of the second hydrogel is disposed around the first volume of the first hydrogel; and (iv) transferring the hydrogel object from the first hydrophobic medium to an aqueous medium and thereby producing the composition comprising the aqueous medium and, disposed in the aqueous medium, the first volume of the first hydrogel. The invention further provides a hydrogel object, which hydrogel object comprises a first volume of a first hydrogel and a second volume of a second hydrogel, which second volume of the second hydrogel is disposed around the first volume of the first hydrogel.

A cell culture matrix is provided that has a substrate with a first side, a second side opposite the first side, a thickness separating the first side and the second side, and a plurality of openings formed in the substrate and passing through the thickness of the substrate. The plurality of openings allow flow of at least one of cell culture media, cells, or cell products through the thickness of the substrate, and provides a uniform, efficient, and scalable matrix for cell seeding, proliferation, and culturing. The substrate can be formed from a woven polymer mesh material that provides a high surface area to volume ratio for cells and good fluid flow through the matrix. Bioreactor systems incorporating the cell culture matrix and related methods are also provided.

The present invention provides a nerve cell device in which early observation of nerve activity (spikes, bursts, and the like) is made possible and the measured electric strength is increased by cultivating neurons upon a cell scaffold. By using this nerve cell device, imaging of intracellular signaling is also possible.

A method for achieving microfluidic perfusion of a spheroid, the method being implemented in a microfluidic device that includes a cavity for hydrodynamically trapping the spheroid, the method including performing a first injection of a gel containing the spheroid into the microfluidic network, hydrodynamically trapping the spheroid in the trapping cavity of the microfluidic network, performing a second injection of a fluid that is non-miscible with the gel into the microfluidic network with a view to flushing away gel present in the network, except in the trapping cavity, cross-linking the gel present around the spheroid, in the trapping cavity, performing a third injection of a culture medium into the microfluidic network with a view to perfusing the spheroid petrified in its gelled environment, and located in the trapping cavity.

An aseptic bioprocess package is provided herein. The aseptic bioprocess package includes a 2D flexible container including an interior compartment, a height having an upper half and a lower half, an inlet and an outlet, the inlet and the outlet being disposed on the same half of the 2D flexible container and a channel-forming feature in the interior compartment of the container, the channel-forming feature being configured to maintain a fluid flow path that fluidly connects the interior compartment of the flexible container with the outlet.

A cell culture system is provided that includes a cell culture vessel having an interior cavity to house a cell culture substrate in a cell culture space, and at least one port for at least one of fluid inlet to the interior cavity and fluid outlet from the interior cavity. The system further includes a piston having a distal end disposed in the cell culture vessel above the cell culture space, the distal end of the piston being sealed with an airtight seal within the interior cavity. The system also includes a driver coupled to the piston to move the piston so as to increase and decrease a distance between the distal end and the cell culture space. The driver can pressurize the interior cavity via actuation of the piston to harvest cells from the cell culture space through the at least one port.

Proposed is a bioink supply system and, more particularly, proposed is a bioink supply system including: a hydrogel storage part; cell storage part; a mixing part configured to receive and mix a hydrogel and a cell solution from the hydrogel storage part and the cell storage part; a sensor part configured to measure a level of bioink inside a syringe; and a controller configured to receive a signal from the sensor part and maintain a constant level of the bioink inside the syringe, in which the mixing part supplies, to the syringe, the bioink prepared by mixing the hydrogel and the cell solution. The bioink supply system can continuously supply an active bioink to a syringe of a bioprinter during 3D bioprinting, and thus can continuously print large-scale biotissue, a plurality of organoids, organ-on-a-chip devices, etc.

A platform for creating engineered tissues includes a vascular tube that defines a vascular diameter and is configured to receive vascular system seed cells, a non vascular tube that defines a non-vascular tube diameter and is configured to receive organ system seed cells, and a barrier formed between the vascular tube and the non vascular tube.

Disclosed is a 3D porous medium and a method of manufacture. The 3D porous medium includes (i) a support structure of transparent hydrogel particles or emulsion droplets, (ii) bacterial nutrient in open volumes between the transparent hydrogel particles, as well as within micropores in the transparent hydrogel particles, and (iii) bacterial cells within the open volumes in the support structure.