This disclosure describes a biochemical reactor with a lower divider support structure. The biochemical reactor may include a tank configured to house immobilized carriers and fluid. The biochemical reactor may include a circulation conduit at least partially disposed within the tank. The circulation conduit may include a circulation outlet opening. The biochemical reactor may include one or more vanes disposed proximate to the circulation outlet opening. The biochemical reactor may include a tank recirculation port disposed proximate to a second end. The biochemical reactor may include a tank inlet configured for feeding fluid into the tank. The biochemical reactor may include a tank outlet configured for drawing fluid from the tank. The tank outlet may be disposed proximate to a first end. The biochemical reactor may include a first divider and a second divider. The second divider may include a support structure including a grating configured to withstand variable loads.

Disclosed herein are flow-through apparatuses for delivering microcarriers, the apparatuses comprising a body having a fixed volume and comprising an inlet, an outlet, and a cavity configured to contain at least one microcarrier; a first seal attached to the inlet and comprising at least one inlet port; a second seal attached to the outlet and comprising at least one outlet port; and at least one conduit attached to the at least one inlet port and/or the at least one outlet port. Systems comprising such flow-through apparatuses in fluid contact with a bioreactor are also disclosed herein. Further disclosed herein are methods for delivering microcarriers into a reactor, the methods comprising placing a delivery apparatus comprising at least one microcarrier in fluid contact with the reactor; and applying a force sufficient to induce flow of the at least one microcarrier into the reactor.

A biopharmaceutical liquid reservoir, includes a bag forming an inner storage space for storing a biopharmaceutical liquid, a mechanical member located at a wall of the bag, having: a stationary set of parts that is stationary with respect to the wall of the bag, a rotating set of parts rotating about an axis of rotation with respect to the stationary set, a bearing located between the two sets and, inside the bag, a communication passage: separating the bearing from the inner storage space, including one or more changes of direction, being formed by a portion of the parts of the rotating set located opposite a portion of the parts of the stationary set.

Disclosed herein are methods of producing one or more cannabinoid compounds, expanding cells that produce the compounds, cannabinoid compounds produced by the methods, pharmaceutical compositions comprising the cannabinoid compounds, and methods of producing and utilizing the compounds and compositions.

An apparatus for separating cells from microcarriers comprises a chamber having a body and a base. The chamber is configured to receive a mixture comprising cells and microcarriers. The apparatus further comprises a filtration element disposed within the chamber and coupled to the body. The filtration element is configured to filter microcarriers from the mixture. The apparatus further comprises an adjustment mechanism coupled to the chamber. The adjustment mechanism is configured for selective extension and retraction of at least a portion of the body of the chamber to move the filtration element relative to the base of the chamber.

The present invention relates to an apparatus for preparing enzyme-immobilized beads used for preparation of tagatose, and a method for preparing enzyme-immobilized beads using the same. More specifically, the present invention relates to an apparatus for preparing enzyme-immobilized beads, comprising a nozzle with an inside diameter of 0.1-1 mm having a cylindrical lower end and comprising a cut-type liquid outlet (cut perpendicularly to the vertical axis of the nozzle) formed at the lower end, and a method for preparing enzyme-immobilized beads using the same.

Disclosed herein is a single use continuous recovery, flow-through harvest vessel and corresponding method for harvesting culture medium and simultaneously either leaving the microcarrier beads behind in the vessel or flowing microcarrier beads and medium back into a bioreactor.

Apparatus, system, and method are disclosed for cell recovery or cell separation. A cell separation system has at least a first container having a first filter for separating a first removal target from a suspension comprising cells and a removal target including at least the first removal target and a second removal target; a cell separation apparatus; a first flow path connecting the first container and the cell separation apparatus; and a first pump interposed in the first flow path for transferring the suspension from the first container to the cell separation apparatus by decompression suction, for example.

This invention relates to compositions of matter, methods, modules and instruments for automated mammalian cell growth and mammalian cell transduction followed by nucleic acid-guided nuclease editing in live mammalian cells.

A filter bag assembly includes: a flexible first sheet; a flexible second sheet overlying and secured to the first sheet so that a compartment is formed therebetween; a porous filter sheet disposed between the first sheet and the second sheet so as to divide the compartment into a first compartment and a second compartment; a first port secured to the second sheet so as to communicate directly with the first compartment; and a second port secured to the second sheet so as to communicate directly with the second compartment, the second port being spaced apart from the first port. The porous filter sheet is disposed so that fluid entering the first compartment through the first port must pass through the filter sheet before entering the second compartment or exiting through the second port.