A bioreactor for culturing of cells is described. Screens suitable as a cell growth scaffold may comprise crossed fibers. Screens may be contained loosely in a screen holder, which in turn may be contained inside a manifold assembly. A lower manifold, screen holder and upper manifold may have identical or similar interior open cross-sections. Flow of liquid medium can occur upwardly through the array of screens, then flowing over a weir in the presence of an air pocket, and into a moat and a pump. The screen holder may have slots whose exterior-facing ligaments are rounded, and may have grooves whose interior-facing edges are rounded. These components may be located inside an incubator suitable to maintain desired environmental conditions and cleanliness.

A bioreactor has a container, an end plate, an agitator, a drive unit, and at least one field device arranged in the interior, field device electronics, a data transmission unit arranged on the end plate, and a motor head piece coupled in terms of energy to the drive unit. At least one communication unit is coupled in terms of signaling to the motor head piece and includes an adapter piece, having adapter electronics which are electrically connected to the field device electronics of the field device, and a communicator piece. The adapter piece and the communicator piece are separate components which can be coupled to one another, wherein the motor head piece is a separate component which can be coupled to the communication units.

A device (30) includes a base connector (32) having an opening (33) and an impeller connector (64) coupled to the base connector (32). The impeller connector (64) has a through-passage (66) aligned with the opening (33) of the base connector (32). Further, the device (30) includes a flexible tube (34) having a first end (36) and a second end (40), where the first end (36) of the flexible tube (34) is coupled to the impeller connector (64). Furthermore, the device (30) includes a seal component (38) and an impeller (42) coupled to the second end (40) of the flexible tube (34). Additionally, the device (34) includes an enclosure (46) disposed enclosing the impeller (42), the flexible tube (34), the impeller connector (64), and the base connector (32).

A multi-chamber single-use bioreactor for cell culture expansion has bag assembly and a rigid support structure defining a bag receiving space. The bag assembly disposed in the bag receiving space of the rigid support structure and supported by the rigid support structure. The bag assembly has at least a first flexible bag and a second flexible bag. The first bag defines a first reaction chamber, and the second bag defines a second reaction chamber. The first reaction chamber has a first volume, a first inlet, and a first outlet, and the second reaction chamber has a second volume different from the first volume, a second inlet, and a second outlet. The second inlet of the second bag is fluidically connected to the first outlet of the first bag so liquid in first reaction chamber can be transferred to the second reaction chamber.

The present invention relates to a process for the production of in vitro engineered tissues, also known in the art as cultured meat, cultivated meat, cell based meat, cellular meat and/or clean meat, and a device for the production of the same. The process comprises the steps of: loading sterilizable 3D scaffolds into a seeding chamber, sterilization of the scaffolds in the seeding chamber, seeding the scaffolds by loading a first volume of culture medium into the seeding chamber, wherein the first volume of culture medium has a density of cells in suspension of 5,000 to 25,000 cell/cm.sup.2, more preferably from 10,000 to 16,000 cm.sup.2 such that the scaffolds are immersed in the culture medium, leaving the scaffolds and the first volume of culture medium in the seeding chamber for a period of 2-24 hours at 18-37° C., loading a second volume of culture medium into a bioreactor, the second volume being greater than the first volume of the culture medium wherein an incubation position of one or more movable grids inside the bioreactor confines the scaffolds to the second volume of the culture medium such that they remain immersed during an incubation step, wherein the scaffolds and the second volume of culture medium are incubated in the bioreactor for a period of 10-60 days at 18-37° C. and at a pH between 6.5-8.0, more preferably 7.0-7.4. The invention also relates to a device for the production of cultured meat.

A system includes a bioprocessing vessel formed of a material insulative to a liquid medium when present therein. At least one sensor is for sensing a parameter of the bioprocessing vessel and generating a signal indicative of the parameter. At least ne conductor is adapted to provide electrical communication between the liquid medium in the bioprocessing vessel and an external structure to achieve equipotential for reducing noise in the signal produced by the at least one sensor. Related apparatus and methods are also disclosed.

Provided herein are systems and methods for thermally separating a flask and the base of an automatic flask stirring device. A thermal separator apparatus may be provided with a stand and a plurality of first standoffs. The stand may have a top surface configured to support a flask containing a substance to be stirred by a flask stirrer. The first standoffs may be located between the stand and a flask stirrer base, thereby creating a first air gap between the stand and the flask stirrer base. In some embodiments, the first air gap allows air to flow straight through the first air gap in a first direction.

Disclosed herein are platforms, systems, and methods including a cell culture system that includes a cell culture container comprising a cell culture, the cell culture receiving input cells, a cell imaging subsystem configured to acquire images of the cell culture, a computing subsystem configured to perform a cell culture process on the cell culture according to the images acquired by the cell imaging subsystem, and a cell editing subsystem configured to edit the cell culture to produce output cell products according to the cell culture process.

An impeller, for example, a Rushton impeller for a bioreactor system is disclosed. The impeller includes a hub, optionaly including a slot, a plurality of blades, and one or more turbulators. The plurality of blades is disposed along a circumferential direction of the hub and spaced apart from each other. Each of the plurality of blades is coupled to at least a portion of a circumference and/or a top surface of the hub. Each blade of the plurality of blades includes a pressure face and a suction face. The one or more turbulators is disposed on at least a portion of the suction face, the pressure face, or both, of a blade of the plurality of blades.

Cells may be cultured by a method including the following steps: a first step of preparing a population of cell aggregates having a major axis of not more than 400 μm, and a second step of suspension culturing the population of cell aggregates obtained in the first step.