According to one embodiment of the present disclosure, a cell culture system includes: a cell culture container; a liquid storage part configured to store a liquid including a culture medium or a reagent to be supplied to the cell culture container; and a cell collection part configured to collect cells cultured in the cell culture container, wherein the cell culture container, the liquid storage part, and the cell collection part are connected by spatially closed-system lines at least during a period from feeding of the liquid to the cell culture container to removal of the cultured cells, and wherein the cell culture container is arranged in an incubator in a form of a multistage shelf including a liquid supply/discharge port.

An automated cell culturing device, which expands, detaches and prepares cells, ready to be implanted in vivo is disclosed. The device is composed by a multi-layered cell culture chamber, a cell preparation chamber, and critical parameters control units which automatically drive the cell culture medium circulation, change and refill. The device according to the invention is characterized in that all the components contacting cells and culture medium constitute a totally disposable “cartridge” in order to avoid cross-contamination and improve safety. The device is particularly useful for expanding and preparing mesenchymal stem cells for osteoarthritis (OA) therapy, and for other cell based therapies in mammals.

A cell culture device includes: a base; a rotary table attached to the base; a container holding member attached to the rotary table and configured to hold a cell culture container; and a drive mechanism configured to rotatably drive the rotary table around a first axis and the container holding member around a second axis, wherein a liquid port to which a liquid tube is connected is arranged on an outer peripheral surface of the cell culture container, wherein a first predetermined portion of the liquid tube is positioned on the container holding member by a first positioning member, wherein a second predetermined portion of the liquid tube is positioned on the rotary table by a second positioning member, and wherein the first positioning member is rotatably attached to the container holding member.

The present invention is a kit to be used for obtaining Adipose tissue-based stem cells, which especially is suitable for obtainment of Adipose tissue-based stem cells, which comprises a reservoir (1) through which the entrance of the adipose tissue, that is taken from a predetermined area of the individual, into the kit; characterized by comprising a first housing (2), which is located on the said reservoir (1) and enables the transmission of the adipose tissue into the kit, a second housing (3), which is located on the said reservoir (1) and which enables the second injector (40) to be positioned outside the reservoir (1) and in a perpendicular position to the reservoir (1), a third housing (4) which enables the third injector (50) to be positioned outside the reservoir (1) and in a perpendicular position to the reservoir (1), an empty body (6) which is fixed inside the reservoir (1), a first inlet (7) which enables the transmission of the adipose tissue into the body (6) by pushing of the injector, a passage (8) which can rotate its own axis and which enables the circulation of the adipose tissue that is inside the body (6) between a desired inlet inside the body (6) and another inlet which is positioned in a 90 degree angle to this inlet by this rotation movement, at least one cover (10) which enables the said movement of the passage (8) and covers the top of the reservoir (1), a second inlet (12) which comprises a first splitting element (11) that is obtained by alignment of a multiple of cutting elements (10), and a third inlet (14) which comprises at least one second splitting element (13) which enables the adipose tissue split when it enters to and exits from the injector and is obtained by alignment of a multiple of cutting elements (10).

The present invention concerns an improved bioreactor with enhanced oxygen transfer. In lieu of the standard impeller and sparger assembly, a rotating gas sparger is used to introduce oxygen into the reactor. A mechanical drive shaft is coupled to a conduit from which a length of tubing extends. A source of pressurized gas is fluidly coupled to the tubing, which tubing is rotated via the mechanical drive shaft. The rotating tubing has a variably sized outlet for introducing oxygen bubbles into the liquid medium of the reactor, the bubbles providing both mechanical agitation and oxygen transfer due to the rotation of the tubing.

Bioreactors and methods of stimulating continuous growth of hairy root biomass in such bioreactors are provided.

Conducting oxidation in an inflatable bag bioreactor (or batch reactor more generally) provides an efficient, economical, and convenient reaction vessel. Rotating or rocking the inflatable bag bioreactor (or batch reactor more generally) during the reaction helps ensure continued exposure of the reaction mixture to the headspace gas in the vessel. The ability of the inflatable bag to expand or contract as the volume of the contents changes helps maintain consistent pressure and avoid the need to replenish the headspace gas.

The disclosure relates to bioreactors, for example for biological treatment and, more specifically to bioreactor insert apparatus including biofilms and related methods. The bioreactor insert apparatus provides a means for circulation of reaction medium within the bioreactor, a biofilm support, and biological treatment of an inlet feed to the reactor/insert apparatus. The bioreactor insert apparatus has a high relative surface area for biofilm attachment and is capable of generating complex flow patterns and increasing treatment efficiency/biological conversion activity in a biologically-active reactor. The high surface area structure incorporates multiple biofilm support structures such as meshes at inlet and outlet portions of the structure. The biofilm support structures and biofilms thereon can increase overall reaction rate of the bioreactor and/or perform some solid/liquid separation in the treatment of the wastewater or other influent.

Apparatus and associated methods relate to an optimizable cellular growth chamber, where growth conditions are optimized by adjusting gas pressures through a gas permeable membrane. In an illustrative example, an apparatus may provide a volume to contain growth medium and the cells under propagation, where the volume has limited but non-zero pneumatic communication with a pressure-controlled chamber via a gas-permeable membrane. Associated apparatus and methods are proposed to manage desired growth conditions via deliberate control of parameters, such as partial pressures, for example.

A multi-layer culture vessel operation system capable of easily performing a multi-layer culture vessel handling operation is provided. A multi-layer culture vessel operation system includes: a cart device 20 movable with a multi-layer culture vessel 30 mounted thereon; and an operational device 10 capable of holding and rotating the multi-layer culture vessel 30, wherein the cart device 20 includes: a cart 21 having wheels 22; and a fixing part 23 that is removably mounted on the cart 21, and fixes the multi-layer culture vessel 30 to the cart, wherein the operational device 10 includes: a rotating portion 11 that holds the multi-layer culture vessel 30 and performs a rotation operation of rotating the multi-layer culture vessel 30 around a first rotation axis and/or a second rotation axis; a shaking portion 13 that holds the multi-layer culture vessel 30 and performs a shaking operation of shaking the multi-layer culture vessel 30 in a horizontal direction; and a control portion 15 that causes the shaking portion 13 to perform the shaking operation following the rotation operation by the rotating portion 11, without returning the multi-layer culture vessel 30 to the cart 21.