A bioreactor configured for stimulation of a plurality of samples includes a well plate containing a plurality of chambers and a cover placed over the well plate. A plurality of fixed arms are located in the cover, each chamber of the plurality of chambers having a fixed arm located therein. A plurality of moving arms are located in the cover, each chamber of the plurality of chambers having a moving arm located therein. The moving arm and fixed arm of each chamber hold a sample therebetween. A moving arm shaft is located in the cover, and is operably connected to the plurality of moving arms. An electrical motor is located outside of the cover and is operably connected to the moving arm shaft. The electrical motor is configured to drive movement of the plurality of moving arms relative to the plurality of fixed arms via the moving arm shaft.

An algal growth system can include a frame and a flexible sheet material that can have a substantially vertical orientation, where the flexible sheet material can be configured to facilitate the growth and attachment of algae, where the flexible sheet material can be supported by the frame. The algal growth system can include a first drive shaft, where the first drive shaft can be coupled with the frame, where the first drive shaft can support and actuate the flexible sheet material, a gear system, where the gear system can be coupled with the first drive shaft, a first roller, where the first roller can be coupled with the frame and can be configured to guide the flexible sheet material, and a drive motor, where the drive motor can be coupled with the gear system, where the drive motor can actuate the gear system and the at least one drive shaft such that the flexible sheet material can be actuated.

The disclosure relates to bioreactors, for example for biological treatment and, more specifically to bioreactor insert apparatus including biofilms and related methods. The bioreactor insert apparatus provides a means for circulation of reaction medium within the bioreactor, a biofilm support, and biological treatment of an inlet feed to the reactor/insert apparatus. The bioreactor insert apparatus has a high relative surface area for biofilm attachment and is capable of generating complex flow patterns and increasing treatment efficiency/biological conversion activity in a biologically-active reactor. The high surface area structure incorporates multiple biofilm support structures such as meshes at inlet and outlet portions of the structure. The biofilm support structures and biofilms thereon can increase overall reaction rate of the bioreactor and/or perform some solid/liquid separation in the treatment of the wastewater or other influent.

Apparatus and associated methods relate to an optimizable cellular growth chamber, where growth conditions are optimized by adjusting gas pressures through a gas permeable membrane. In an illustrative example, an apparatus may provide a volume to contain growth medium and the cells under propagation, where the volume has limited but non-zero pneumatic communication with a pressure-controlled chamber via a gas-permeable membrane. Associated apparatus and methods are proposed to manage desired growth conditions via deliberate control of parameters, such as partial pressures, for example.

Assay and systems are provided that include use of cells and/or organoid for patient diagnosis, drug development, and personalized medicine, including for diagnosis and treatment of cancer and associated diseases and disorders. In one aspect, systems and methods include applying a phase-specific and force-guided polymerization of a biomaterial, wherein the polymerization comprises a nucleation phase and an elongation phase; thereby, producing a model.

Provided is a cell culture device capable of mass culturing stem cells while reducing occurrence of karyotypic abnormalities due to an influence of magnetism. The cell culture device includes: a cell culture vessel that is a substantially cylindrical body having a flat bottom at a lower end; a dish-shaped body having, in a periphery, a plurality of first magnetic bodies at equal intervals, the dish-shaped body having a substantially disk shape and disposed horizontally in a hollow space of the cell culture vessel without contact; and an annular body having a plurality of magnetic bodies and positioned outside the cell culture vessel to have the cell culture vessel located inside a ring of the cell culture vessel. The annular body and the dish-shaped body move up and down in conjunction with each other due to a magnetic force to stir a medium and supply nutrients to the cells. The cell culture device includes a storage made of a ferromagnetic material and configured to store the annular body when the annular body moves downward below the bottom of the cell culture vessel.

An algal growth system including a first flexible sheet material mounted in a first mounted geometry such that the height is greater than the width of the first mounted geometry, and a second flexible sheet material mounted in a second mounted geometry such that the height is greater than the width of the second mounted geometry, the first flexible sheet and the second flexible sheet material being noncontiguous, a motor, the motor being coupled with an actuator system, where the motor actuates the actuator system, and a reservoir.

A fluidic system for producing extracellular vesicles from producer cells, including at least one container, a liquid medium contained by the container and producer cells, characterised in that it also includes microcarriers suspended in the liquid medium, the majority of producer cells being adherent to the surface of the microcarriers, and a liquid medium agitator, the agitator and the dimensions of the container being adapted to control a turbulent flow of the liquid medium in the container.

The present disclosure relates to cell cultured adipose tissue. In one embodiment, the cultured adipose tissue is produced by culturing adipose cells in a culture media in vitro, harvesting the adipose cells after a desired amount of adipose cells are produced, and aggregating the harvested adipose cells to provide the cultured adipose tissue. In some embodiments, aggregating the harvested adipose cells comprises mixing the harvested adipose cells with a hydrogel or binder in a three-dimensional (3D) mold. In other embodiments, aggregating the harvested adipose cells comprises cross-linking the harvested adipose cells in a 3D mold. The cultured adipose tissue have a defined 3D shape and a size on the macroscale. In some embodiments, the cultured adipose tissue may be a food product.

An automated cell culturing device, which expands, detaches and prepares cells, ready to be implanted in vivo is disclosed. The device is composed by a multi-layered cell culture chamber, a cell preparation chamber, and critical parameters control units which automatically drive the cell culture medium circulation, change and refill. The device according to the invention is characterized in that all the components contacting cells and culture medium constitute a totally disposable cartridge in order to avoid cross-contamination and improve safety. The device is particularly useful for expanding and preparing mesenchymal stem cells for osteoarthritis (OA) therapy, and for other cell based therapies in mammals.