A multi-layer culture vessel operation system capable of easily performing a multi-layer culture vessel handling operation is provided. A multi-layer culture vessel operation system includes: a cart device 20 movable with a multi-layer culture vessel 30 mounted thereon; and an operational device 10 capable of holding and rotating the multi-layer culture vessel 30, wherein the cart device 20 includes: a cart 21 having wheels 22; and a fixing part 23 that is removably mounted on the cart 21, and fixes the multi-layer culture vessel 30 to the cart, wherein the operational device 10 includes: a rotating portion 11 that holds the multi-layer culture vessel 30 and performs a rotation operation of rotating the multi-layer culture vessel 30 around a first rotation axis and/or a second rotation axis; a shaking portion 13 that holds the multi-layer culture vessel 30 and performs a shaking operation of shaking the multi-layer culture vessel 30 in a horizontal direction; and a control portion 15 that causes the shaking portion 13 to perform the shaking operation following the rotation operation by the rotating portion 11, without returning the multi-layer culture vessel 30 to the cart 21.

A method, and also a gas supply system without a separate humidifying apparatus, for supplying gas to a plurality of bioreactors, divides a constant gas stream with high distribution accuracy into a plurality of gas substreams having a mandated volume flow, which can be kept constant at the mandated level even when during gas supply there is fluctuation in the opposing pressure in the gas line to the respective bioreactor, and decouples a gas distribution from the opposing pressure by hydrostatic pressure compensation, with the gas distribution at the same time producing an obligatory humidification of the gas stream.

Systems and methods for automated cell culturing are disclosed. In some embodiments, one or more cell culture vessels are fluidly connected with one or more multiport valves and one or more fluid pumps. The fluid pumps may pump various fluids into and out of the cell culture vessels as necessary to support cell growth, routed by the one or more multiport valves. In some embodiments, one or more components may be removable from other components so that some components may be prepared and sterilized independently prior to usage.

An integrated modular microphysiological system is provided which includes a two or more chambers and a vascular network which includes at least one channel. The chamber can be configured for culturing a tissue and includes a layer of endothelial cells which forms an endothelial barrier within the well. The endothelial barrier can be in fluid contact with at least one of the at least one channels in the vascular network. The endothelial barrier can also be in fluid contact with a fluid in the chamber.

The present disclosure generally pertains to a biomimetic array device and methods of using the device to expose biological samples to an array of fluids. The device includes a cassette and an inlet region, where the cassette comprises at least one microchamber array and at least one microchannel. Each microchamber within a microchamber array has a top interface that is open to the external environment, so that a biological sample placed at the top interface is positioned to draw fluid from the microchambers. The inlet region comprises at least one well and at least one inlet channel, each well in fluid communication with one inlet channel. Fluid deposited into wells flows through each inlet channel and microchannel in fluid communication with each well containing fluid, so that each microchamber within one microchamber array provides an approximately equal volume of fluid to the biological sample.

The invention concerns a cassette for cell culture comprising: an at least partially rigid housing internally defining an internal space inside which a cell culture bag defining an internal volume is arranged and attached; conduits each connected at one end to the internal volume of the bag and each having a second end situated outside the housing; and valves for enabling/preventing the flow of fluid through the conduits that are mounted on the housing.

The present invention is a resonant vibratory agitation mechanism for installing inside bioreactor containers for agitating and degrading biodegradable waste. It either comprises of a sole layer of horizontally arranged springs with at least one vibration motor installed inside each of the springs, or comprises of a central frame, a plurality of vibration motors fixed on the central frame and a plurality of layers of horizontally or diagonally arranged extension springs. It provides sound waves, vibrations, resonant vibratory frequencies and heat for agitating and degrading biodegradable waste inside a bioreactor container. It saves costs to fabricate a bioreactor container by assembling a plurality of cylindrical drum barrels on top of a receiving tank. A closed-loop recirculation of water, heat, nutrients, O.sub.2 and CO.sub.2 may be established by integrating the present bioreactor system with wicking beds, hydroponics/aeroponics growing beds, a stove unit and a greenhouse.

The present invention provides a full-automatic cell production line, comprising a culture region and an operation region. The culture region comprises a B-stage platform body, and the B-stage platform body comprises a culture area, a refrigeration area, and a robot provided with a motion track. The motion track of the robot is linearly arranged. The culture area, the refrigeration area, and a manual delivery window all surround the track, and are all located in the operation region of the robot. The operation region is integrated with a liquid storage table, a cell factory liquid changing device, and a centrifugal bottle liquid changing device. The delivery window is used between the culture region and the operation region to deliver a material. The technical solution of the present invention is mainly used for full-automatic batch production of cells.

A device for culturing cells in a shaking operation with passive gas exchange, which includes at least one gas exchange opening, characterized in that the opening surface of the gas exchange opening is not oriented parallel with the movement vector of the shaking movement at at least one point in time of the shaking movement. Owing to the shaking movement and the ambient gas phase flowing around the outside of the device and the headspace gas phase flowing around the inside of the device, which flowing is associated with the shaking, the arrangement leads to the formation of a pressure gradient between the headspace gas phase and the ambient gas phase, which pressure gradient changes depending on the shaking movement and advantageously influences the gas exchange between the headspace gas phase and the ambient gas phase and the gas blending within the headspace.

Bioreactor bags formed from sheet materials are disclosed. The bioreactor bags include an opening which is openable and recloseable in a fluid tight manner for providing an opening for insertion of solid materials into the bag.