A system and method for determining a trajectory parameter of particles, comprising receiving a plurality of particles at a microfluidic channel, applying a force to each particle of the microfluidic channel, acquiring a dataset of each particle, measuring a trajectory of the particle, and determining a trajectory parameter of the particles.

An apparatus and method to maintain pH within a range conducive for cell growth in a bicarbonate-containing cell culture system without the addition of base. The method relies on the gas transfer characteristics of the bioreactor system to modulate the CO.sub.2 transfer to and from the cell culture such that the pH of the cell culture can be maintained within a desired range.

A method for mixing a fluid includes: dispensing a first volume of a fluid into a flexible container, the flexible container being at least partially disposed within the chamber of a support housing; repeatedly moving the support housing and the flexible container contained therein so as to mix the first volume of fluid within the flexible container; adding further fluid into the flexible container after moving the support housing to form a second volume of fluid; and manipulating a mixing element within the flexible container so as to mix the second volume of fluid.

A portable apparatus for transport and incubation of a medical sample in a blood culture flask includes a sealable container having a thermally insulated compartment for receiving the blood culture flask and a heater for heating the medical sample to a temperature suitable for pre-culturing of the sample. An agitator is provided for agitating the sample in the blood culture flask.

A filter holding device (1; 41; 75; 81) arranged for providing at least one filter (7; 43a, 43b; 85) inside a flexible bag (3; 71), said filter holding device comprising attaching means (19a-d, 21a-d; 79; 83) for attaching the filter holding device to an inner surface of the flexible bag such that the at least one filter held by the filter holding device is provided at a distance from the inner surface of the flexible bag.

Provided is a cell culture device that has a simple structure and ensures sufficient nutrient supply to cells and oxygenation of the cells to thereby enable mass cell culture. The cell culture device comprises: a cell culture container which is an approximately cylindrical body provided with a flat bottom part at the lower end; a dish-shaped body having an approximately disc shape which is provided with a plurality of magnetic attraction members, said magnetic attraction members being positioned in the circumferential part at equal intervals, and horizontally disposed in a non-contact state within a hollow space inside the cell culture container; and a cyclic body which is provided with a plurality of magnetic attraction members and positioned outside the cell culture container so that the cell culture container is located within the cycle thereof. The cyclic body and the dish-shaped body move vertically in magnetic conjunction to thereby agitate a medium and supply nutrients to cells.

The invention discloses an immunoglobulin-binding magnetic bead, comprising a porous matrix and one or more magnetic particles embedded in said matrix, wherein said matrix comprises a porous polymer and at least 10 mg/ml Fc-binding proteinaceous ligands covalently coupled to said porous polymer.

Aspects of the present disclosure include methods of producing a cellular suspension from a tissue sample by applying resonant acoustic energy to a container comprising the tissue sample in a manner sufficient to produce a cellular suspension from the tissue sample. Resonant acoustic mixers and kits for use in producing a cellular suspension from a tissue sample are also provided.

Disclosed is biomanufacturing apparatus 1 comprising a housing 20 including top 22 and bottom 24 faces which allow stacking of plural housings, an access door 25 at a front side of the housing, a substantially enclosed bioreactor chamber 30 inside the housing accessible via the door, and a further substantially enclosed region 36 inside the housing containing electrical parts and/or electronic control components, the chamber 30 including: a tray 40 for supporting a bioreactor, a tray support 45 including a mechanism 44,47 for rocking the tray in use; the tray support further including a load cell (41) to determine changes in the mass load on the tray.

Disclosed is biomanufacturing apparatus 1 comprising a housing 20 including top 22 and bottom 24 faces which allow stacking of plural housings, an access door 25 at a front side of the housing, a substantially enclosed bioreactor chamber 30 inside the housing accessible via the door, and a further substantially enclosed region 36 inside the housing containing electrical parts and/or electronic control components, the chamber 30 including: a tray 40 for supporting a bioreactor, a tray support 45 including a mechanism 44,47 for rocking the tray in use; at least a fluid handling portion 48,49 of at least one fluid pump device 54/58; and at least one connection 43 for connecting to a conduit and/or path for carrying fluids, gas and/or electricity.