A cell detector and detecting method enhance detection sensitivity for microbes according to the ATP method and reduce the detection period. The cell detector includes a sealing container having a culture portion for containing a culture medium containing the specimen, a sealable specimen introducing portion, and a luminescent reagent portion that emits light upon contact with the culture medium, a contact mechanism for controlling the contact between the culture medium and the luminescent reagent portion, a photodetector for detecting a luminescence resulting from the contact between the culture medium and the luminescent reagent portion, and a calculation unit for calculating a luminescent quantity from detection signals of the photodetector to determine cell proliferation on the basis of change in the luminescent quantity over time. The culture portion and the luminescent reagent portion are separately positioned, and the contact mechanism intermittently brings the culture medium and the luminescent reagent into contact.

Provided is a culture bag accommodating a culture fluid, the culture bag is capable of suppressing foaming in the culture fluid when oscillating the culture fluid to perform a culture, and performing a culture with high efficiency. The culture bag includes a culture space accommodating a culture fluid, the culture space being an endless space to allow the culture fluid to circulate therein, in which the culture bag has an inner surface that comes into contact with the culture fluid to be accommodated therein, the inner surface including, at least in part thereof, a concave-convex region having a concave-convex structure.

The invention relates to a laboratory tempering device for storing laboratory samples at a target temperature. It relates in particular to an incubator (1) for growing cell cultures. The invention moreover relates to a method for setting a target temperature in a laboratory tempering device that is based in particular on estimating the temperature inside the chamber.

A method of carrying out a bioreaction in a multivessel bioreactor system may include flowing a culture medium at an initial flow rate through one or more fluidic paths between a master vessel and one or more slave vessels, detecting a first culture medium parameter value in the master vessel or the slave vessels, transmitting the first culture medium parameter value to a control system, determining an adjusted flow rate of the culture medium at the control system based on the first culture medium parameter value, and controlling a bidirectional fluid transfer device with the control system to adjust the flow rate of the culture medium through the one or more fluidic paths. The control system may adjust the flow rate of the culture medium to maintain a substantially uniform value of the first culture medium parameter value in the master vessel or the one or more slave vessels.

The invention provides a system for observation of media dissolution and/or bacterial growth in a transparent bag, comprising a receptacle for supporting the bag, and a light emitting device arranged to transmit light into and/or through the interior of the bag supported at the receptacle. The media in the transparent bag can be visually inspected to confirm the dissolution state of the media and check for residual undissolved media and/or confirm the absence of unexpected microbial growth without having to move or handle the bag as it can remain supported in a defined orientation at the receptacle during repeated visual inspections by means of the light emitted from the light emitting device

A process for isolating cells of interest from a sample comprising polluting cells. The process includes a filtration step through a membrane provided with pores and with linkers, and a crosslinking step allowing to bind the polluting cells to the membrane, leaving the cells of interest unbound.

A probe assembly and a method of manufacturing a probe assembly. In one aspect there is a method of manufacturing a probe assembly comprising providing an electrode carrier carrying a plurality of electrodes, the electrode carrier comprising a top wherein the plurality of electrodes are exposed relative to the top and a bottom having a plurality of electrical contacts in electrical communication with the plurality of electrodes respectively; moulding a body around the electrode carrier to retain the electrode carrier whilst leaving the plurality of electrodes exposed. The invention also extends to a biomass monitoring system comprising a flexible enclosure including a probe assembly and support arrangement for receipt of the probe assembly in an engaged configuration.

Aspects of the present disclosure include methods of producing a cellular suspension from a tissue sample by applying resonant acoustic energy to a container comprising the tissue sample in a manner sufficient to produce a cellular suspension from the tissue sample. Resonant acoustic mixers and kits for use in producing a cellular suspension from a tissue sample are also provided.

An apparatus and method to maintain pH within a range conducive for cell growth in a bicarbonate-containing cell culture system without the addition of base. The method relies on the gas transfer characteristics of the bioreactor system to modulate the CO.sub.2 transfer to and from the cell culture such that the pH of the cell culture can be maintained within a desired range.

A method for enhancing mixing and aeration of a liquid reaction medium in a toroidal bioreactor vessel includes dispensing a liquid reaction medium into an interior of a toroidal bioreactor vessel, the interior being bounded by an inner surface, a textured surface being arranged on at least a portion of the inner surface, the textured surface having a plurality of upstanding protuberances. The toroidal bioreactor vessel is rotated in an orbital motion such that there is a resonant frequency traveling wave of the fluid orbiting in one direction in the interior of the toroidal bioreactor vessel when a particular orbital speed is imparted to the toroidal bioreactor vessel.