The invention discloses a bioreactor 10 for cell culture and expansion, comprising a bioreactor chamber 17, a filter 13 disposed within the chamber 17 and at least one tether 14 loosely tethering the filter to the bioreactor chamber. In an embodiment, the tether(s) allow the filter 13 to move from side to side and/or up and down within the chamber 17, preferably without touching an inner surface of the bioreactor chamber 17. Alternatives show, two, three or four tethers, but one or more tethers can be used.

Provided is a biomimetic chip device. The biomimetic chip device includes a body, a main channel arranged in the body and extending in one direction, a plurality of culture chambers spaced apart from each other on the main channel, a first reservoir arranged at one end of the main channel and storing a first fluid, and a second reservoir arranged at the other end of the main channel and storing the first fluid, wherein the body is tilted about a first axis perpendicular to the one direction to allow the first fluid to flow between the first reservoir and the second reservoir.

A culture apparatus includes a culture vessel including an internal space containing a culture solution in which a culture target is suspended, a gas supply apparatus supplying a single gas or a mixed gas to the internal space of the culture vessel, and an agitation apparatus agitating the culture solution by changing position and posture of the culture vessel. The agitation apparatus changes the position and posture of the culture vessel to circulate the culture solution in the internal space of the culture vessel so that a bottom surface of the internal space of the culture vessel is partially revealed and that the revealed portion transitions in a circulating direction R.

The invention provides easy-to-scale methods of creating DNA/transfection reagent master mixes for transfecting cells in culture.

A method for culturing a microbial or cellular seed culture includes positioning a first flexible bag within a chamber of a first retainer, the first retainer being secured to a mixer table. A culture is produced within a compartment of the flexible bag, the culture comprising a growth media and a microbial or cellular starter culture added thereto. The mixer table is activated so that the culture is mixed within the compartment of the first flexible bag while the microbes or cells of the culture grow, the culture having a maximum volume of less than 10 liters.

The invention discloses a flexible bag assembly for cultivation of cells, comprising one or more bags forming a plurality of cultivation compartments, wherein a drain port in at least a first cultivation compartment is adapted to be fluidically connected with a second cultivation compartment upon opening of a valve means. It also discloses a bioreactor with the bag assembly mounted on a rocking tray and a method of cultivating cells in the assembly.

A culturing apparatus (10) is an apparatus for culturing a culture medium with which an airtight container (35) is filled. Such a culturing apparatus (10) is provided with a container holding unit (12) that holds the airtight container (35) and a motion imparting unit (15) that imparts physical motions from the outside of the airtight container (35) to the airtight container (35) held on the container holding unit (12).

A separation system, a method in a separation system and an elution arrangement to be provided in a separation system for separating a biomolecule from a cell culture are provided. The method comprises the steps of: providing a feed from a cell culture (3; 103; 203) comprising said biomolecule to a magnetic separator (5; 105; 205) and providing to the magnetic separator magnetic beads comprising ligands capable of binding this biomolecule; separating by the magnetic separator said magnetic beads with bound biomolecules from the rest of the feed; forwarding said magnetic beads as a slurry with an added buffer to an elution cell (7; 107; 207); eluting the bound biomolecules in the elution cell.

The present invention relates to a microfluidic hanging drop culture device for culturing cell aggregates, and organoids/spheroids cultured in the device of the present invention have greater stem cell activity and higher differentiation than organoids/spheroids of a conventional culture technology. The present invention can be reused repeatedly, and can be utilized as platforms according to various uses by changing the size and number of wells, and by using a rocker in the device, a culture solution located in wells in a reservoir and a culture chamber is allowed to continuously flow through microchannels so that the environment of all of the wells is maintained to be the same, and thus cell aggregates can be cultured with high efficiency. In addition, the device for culturing cell aggregates can be used as a model for disease research and drug screening through the mass-production of cell aggregates of which disease phenotypes are maintained, and can also be used in transplantation therapy for treating diseases through the mass-production of therapeutic cell aggregates.

A rocking mechanism for a bioreactor vessel includes a base, a motor mounted to the base and having an eccentric roller driven by the motor, and a rocking plate in contact with the eccentric roller, the rocking plate being configured to receive a bioreactor vessel thereon. The motor is controllable to drive the eccentric roller to transmit a force against an underside of the rocking plate to tilt the rocking plate and bioreactor vessel.