The present invention provides a parallel bioreactor system, comprising: an oscillator for generating oscillating motion; a plurality of culture vessels mounted on the oscillator, wherein each culture vessel is provided with an inner cavity, the inner cavity comprises a cylindrical portion at the upper part and an inverted truncated conical bottom at the lower part, a cross section of the cylindrical portion is consistent with the cross section of the top of the inverted truncated conical bottom, and the bottom of the cylindrical portion is joined with the top of the inverted truncated conical bottom; disposable culture bags arranged in the inner cavities of the culture vessels and used for accommodating culture solution, wherein each disposable culture bag is provided with a multifunctional cover plate, and the multifunctional cover plate is connected to the top of the culture bag to seal the culture bag, and is provided with a plurality of connection holes leading to interior of the disposable culture bag; and a control system, wherein the control system controls the oscillating motion of the oscillator and parameters of the culture solution in the disposable culture bags.

Provided is a cell culture system capable of mixing a medium for culturing animal or plant cells. The cell culture system includes a body, a movable plate including an accommodation space accommodating a culture bag accommodating a medium to mix the medium, and being tiltable in multiple directions by a central joint mounted at a first location of the body, a first actuator mounted between the body and a second location of the movable plate to move the movable plate, a second actuator mounted between the body and a third location of the movable plate to move the movable plate, and a controller for applying a control signal to the first and second actuators, wherein a first angle between a first reference line extending from the first location to the second location, and a second reference line extending from the first location to the third location is 120°.

A separation system, and an elution arrangement to be provided in a separation system, for separating a biomolecule from a cell culture are provided.

Provided are methods for the creation of ultra-high density cryopreserved cell banks. In certain embodiments, these methods employ altered perfusion culture techniques that allow for production of ultra-high density cell cultures that can be cryopreserved at unexpectedly high cell densities without the need for any cell concentration steps, while retaining excellent cell viability and quality.

A method for constructing a slow microcyclic artificial cell niche. A cell niche (62) which is isolated from a flow field is provided in the center of the flow field and the cell niche (62) is in communication with the flow field by means of an opening (61), wherein the opening (61) faces a wake (63) formed by means of the flow field flowing around the cell niche (62).

Growth methods and systems herein include a growth container with one or more meshes attached thereto. The growth container and its meshes can hold a substrate. The growth container and its meshes can be vertically oriented to allow fungal biomass (e.g., mycelium, fruiting body, primordia, etc.) to grow from the substrate and through the meshes. The growth containers can be cylindrical or a rectangular box having meshes extending along vertically oriented longitudinal sides. The meshes may be provided on two opposite sides of the growth cylinders. These meshes can expose the substrate to a growth environment to facilitate growth of the fungal biomass.

An automated cell culturing device, which expands, detaches and prepares cells, ready to be implanted in vivo is disclosed. The device is composed by a multi-layered cell culture chamber, a cell preparation chamber, and critical parameters control units which automatically drive the cell culture medium circulation, change and refill. The device according to the invention is characterized in that all the components contacting cells and culture medium constitute a totally disposable “cartridge” in order to avoid cross-contamination and improve safety. The device is particularly useful for expanding and preparing mesenchymal stem cells for osteoarthritis (OA) therapy, and for other cell based therapies in mammals.

Proposed are a three-dimensional structuring method of cells and a three-dimensional structuring system of cells capable of efficiently bonding multiple cell clusters in a three-dimensional direction, pursuant to their growth, while ensuring safety. A plurality of fibers, in which one end of each of the fibers is held by a flat plate, are inserted together with the flat plate into a flow path through which a culture solution is supplied, a plurality of cell clusters are placed in the flow path upon causing the cell clusters to run with a liquid flow of the culture solution, and each of the cell clusters is cultured by being stacked on an outer surface of each of the fibers with the flat plate as a growth origin.

The present disclosure provides an in vitro life culture system, comprising: a culture module used to cultivate a culture, the culture module including at least a culture chamber for holding a culture fluid; a culture fluid provision module used to provide the culture fluid to the culture module; and a fluid output module used to discharge the culture fluid from the culture chamber.

Embodiments described herein generally provide for expanding cells in a cell expansion system. The cells may be grown in a bioreactor, and the cells may be activated by an activator (e.g., a soluble activator complex). Nutrient and gas exchange capabilities of a closed, automated cell expansion system may allow cells to be seeded at reduced cell seeding densities, for example. Parameters of the cell growth environment may be manipulated to load the cells into a particular position in the bioreactor for the efficient exchange of nutrients and gases. System parameters may be adjusted to shear any cell colonies that may form during the expansion phase. Metabolic concentrations may be controlled to improve cell growth and viability. Cell residence in the bioreactor may be controlled. In embodiments, the cells may include T cells. In further embodiments, the cells may include T cell subpopulations, including regulatory T cells (Tregs), helper, naïve, memory, or effector, for example.