The device for a cell suspension culture comprises a culture chamber comprising first and second axisymmetric-shaped walls having at least a partially curved radial section the concavity thereof facing the inside of the culture chamber, the walls delimiting an inner compartment and form an inner continuous surface; an inlet opening provided in the first wall and adapted to enable a fluid to enter; a supplying conduit connected to the inlet opening; and a valve member disposed inside the culture chamber adapted to tightly close or open the inlet opening. The device comprises also a device for moving the valve member, disposed outside the culture chamber.

An integrated system to produce microalgae with autonomous fault identification and circumvention that can be deployed on land or at sea. The system comprises a fully scalable reactor, CO2 extraction, oxygen replenishment to surrounding water, and all necessary equipment to run it safely ensuring high energy efficiency and optimal control of environment variables to maximize biomass yield.

A bioprocess system and a method for incubating, growing and harvesting cell cultures is described. Also disclosed is a bioprocess container that can be used with the system. In one aspect of the present disclosure, the bioprocess system includes bioprocess tubes and cell culture tubes having particular dimensions and being made from specific materials that allow the tubes to be welded together while preventing open connections and/or ruptures. In this manner, bioprocess containers can be connected and disconnected from a cell culture apparatus without having to perform the manipulation within a closed environment and without associated monitoring.

A trough reactor, including an elongated trough shaped enclosure, a stock feed inlet, a reactant feed-in and distribution system, at least one separation plate extending downwardly underneath the elongated trough shaped enclosure and at least one separation column forming a continuous gravitational decanter with at least one outlet and a posterior outlet, as well as a method of catalyzing a reaction in a trough reactor, including horizontally inclining an elongated trough shaped enclosure, supplying a feed of substrate into the elongated trough shaped enclosure, supplying and distributing a feed-in of a reactant into the elongated trough shaped enclosure; disposing a separation plate essentially vertically in the elongated trough shaped enclosure, substantially obstructing a spontaneous gravitational flow along the elongated trough shaped enclosure by the separation plate, providing a separation column of an essentially hollow vertical structure, separating the substrate or reactant or a product by a means of continuous gravitational decantation process, draining from an outlet at a bottom portion of the separation column an excessive portion of the substrate or reactant or a product and draining a portion of the substrate or reactant or a product from a posterior outlet, are described.

The present application belongs to the technical field of biology. Provided is a photobioreactor used for algae cultivation, said photobioreactor comprising: a reactor main body, a separation unit, and a first aeration device. The reactor main body is a sealed irregular tubular shape, the separation unit is located within the reactor main body, and divides the reactor main body into two spaces, a left space and a right space, and the first aeration device is connected to a bottom portion of the reactor main body. Also provided is an algae cultivation system, comprising the photobioreactor, the second aeration device, and a temperature control system, and being capable of regulating the temperature of an algae solution.

A system and method for growing and maintaining biological material including producing a protein associated with the tissue, selecting cells associated with the tissue, expanding the cells, creating at least one tissue bio-ink including the expanded cells, printing the at least one tissue bio-ink in at least one tissue growth medium mixture, growing the tissue from the printed at least one tissue bio-ink, and maintaining viability of the tissue.

Microalgae cultivation equipment for the cultivation of microalgae is provided in which a raceway is modified so as to contain multiple generally upright photobioreactor columns spaced apart along its length so as to increase the total surface area of liquid growth medium directly exposed to light and to improve the transfer of CO.sub.2 from the gas-phase to the liquid-phase by providing adequate height inside the vertical photobioreactor columns. The lowermost ends of the photobioreactor columns are immersed inside the liquid growth medium in the raceway component and are fed with liquid growth medium by a circulation promoting facility circulating the liquid growth medium from the raceway through the photobioreactor columns to become discharged back into the raceway. Gas inlets provide CO.sub.2 containing gas bubbles passing upwards in each of the photobioreactor columns. One or more paddle wheels or jet pumps induce a flow of liquid growth medium within the raceway.

Methods for introducing exogenous material into a cell are provided, which include exposing the cell to a transient decrease in pressure in the presence of the exogenous material. Also provided are devices for performing the method of the invention.

A system and method for growing and maintaining biological material including producing a protein associated with the tissue, selecting cells associated with the tissue, expanding the cells, creating at least one tissue bio-ink including the expanded cells, printing the at least one tissue bio-ink in at least one tissue growth medium mixture, growing the tissue from the printed at least one tissue bio-ink, and maintaining viability of the tissue.

A device for treatment of cells with particles is disclosed. The device includes a semi-permeable membrane positioned between two plates, the first plate defining a first flow chamber and comprising a port, a flow channel, a transverse port, and a transverse flow channel, the first flow chamber constructed and arranged to deliver fluid in a transverse direction along the first side of the semi-permeable membrane, the second plate defining a second flow chamber and comprising a port. A method for transducing cells is disclosed. The method includes introducing a fluid with cells and viral particles into a flow chamber adjacent a semi-permeable membrane such that the cells and the viral particles are substantially evenly distributed on the semi-permeable membrane. The method also includes introducing a recovery fluid to suspend the cells and the viral particles, and separating the cells from the viral particles. A method of activating cells is disclosed.