Methods and systems are provided for transfecting cells using real-time, continuous transfection of cells. In some aspects, the methods can be applied for the continuous production of non-viral vector nucleic acid complexes. The systems and methods include a passive mixing fluidic module with at least two inlets, a plurality of mixing elements, and an outlet to provide a continuous flow of transfection complexes to a cell reactor. The transfection agent and nucleic acid are passively mixed and then provided to cells in a continuous flow of cell medium. In some aspects, the flow of cell medium perfusing through the cell reactor recirculates. The system and the methods of the present disclosure provide for highly reproducible and scalable transfection with a low coefficient of variation.

An apparatus for managing gas bubbles in a bioprocessing system includes a body portion having a underside surface, and an opening in the body portion. The body portion is configured for placement within a bioreactor vessel such that the underside surface of the body portion is disposed in a liquid within the bioreactor vessel. The underside surface of the body portion is configured to divert rising gas bubbles in the liquid towards the opening.

A device for treatment of cells with particles is disclosed. The device includes a semi-permeable membrane positioned between two plates, the first plate defining a first flow chamber and comprising a port, a flow channel, a transverse port, and a transverse flow channel, the first flow chamber constructed and arranged to deliver fluid in a transverse direction along the first side of the semi-permeable membrane, the second plate defining a second flow chamber and comprising a port. A method for transducing cells is disclosed. The method includes introducing a fluid with cells and viral particles into a flow chamber adjacent a semi-permeable membrane such that the cells and the viral particles are substantially evenly distributed on the semi-permeable membrane. The method also includes introducing a recovery fluid to suspend the cells and the viral particles, and separating the cells from the viral particles. A method of activating cells is disclosed.

Microalgae cultivation equipment for the cultivation of microalgae is provided in which a raceway is modified so as to contain multiple generally upright photobioreactor columns spaced apart along its length so as to increase the total surface area of liquid growth medium directly exposed to light and to improve the transfer of CO.sub.2 from the gas-phase to the liquid-phase by providing adequate height inside the vertical photobioreactor columns. The lowermost ends of the photobioreactor columns are immersed inside the liquid growth medium in the raceway component and are fed with liquid growth medium by a circulation promoting facility circulating the liquid growth medium from the raceway through the photobioreactor columns to become discharged back into the raceway. Gas inlets provide CO.sub.2 containing gas bubbles passing upwards in each of the photobioreactor columns. One or more paddle wheels or jet pumps induce a flow of liquid growth medium within the raceway.

A bioreactor can include: a tissue culture chamber; at least one inlet port into the tissue culture chamber; an inlet port member located in each inlet including an inlet tube extending into the tissue culture chamber; at least one outlet port into the tissue culture chamber; an outlet port member located in each outlet including an outlet tube extending into the tissue culture chamber; an optical cover; and a hydrogel can be located in the tissue culture chamber having at least one lumen fluidly coupling the inlet tube to the outlet tube, wherein an inlet interface region of the hydrogel is constrained around the inlet tube and an outlet interface region of the hydrogel is constrained around the outlet tube.

Provided herein according to some embodiments is an in vitro construct useful as a model for a hematopoietic microenvironment, which may include: a microfluidic device having multiple chambers; and two or more populations of cells (e.g., 3 or 4 populations of cells) (or “niches”) selected from: 1) mesenchymal cells (e.g., Stro-1+; MSC); 2) osteoblasts (OB; optionally said osteoblasts provided by differentiating mesenchymal cells to differentiated osteoblasts); 3) arterial endothelium (e.g., CD146+NG2+; AEC); and 4) sinusoidal endothelium (CD146+NG2−; SEC), wherein each of said two or more populations of cells are provided in a separate chamber of the microfluidic device. Methods of making and using the construct are also provided.

A system and method for growing and maintaining biological material including producing a protein associated with the tissue, selecting cells associated with the tissue, expanding the cells, creating at least one tissue bio-ink including the expanded cells, printing the at least one tissue bio-ink in at least one tissue growth medium mixture, growing the tissue from the printed at least one tissue bio-ink, and maintaining viability of the tissue.

A preparation system for preparing a cell suspension having a carrier on which a reactor housing and a magnet system are accommodated, wherein, in the reactor housing, a reaction channel is formed, which extends between an inlet opening arranged centrally on an upper side of the reactor housing and an outlet opening arranged on the outside of the reactor housing and which is bounded by a channel wall, the magnet system being received on the carrier so as to be relatively movable between a first functional position, to bear with a pole face against the channel wall of the reaction channel and a second functional position to assume with the pole face a predetermined distance relative to the channel wall, and having a reactor housing drive for initiating a rotational movement on the reactor housing.

A perfusion cell culture device includes a driving module and a plurality of cell culture modules. The driving module includes a driving source connecting opening and a chamber. The chamber and the driving source connecting opening are connected. Each of the culture modules includes a fluid channel, a first elastic element, two flow direction controlling units and a cell culture zone. The fluid channel is disposed above the chamber, the first elastic element is disposed between the fluid channel and the chamber, the two flow direction controlling units are respectively disposed on two ends of the fluid channel and connected to the fluid channel selectively, and the cell culture zone is connected to the two flow direction controlling units.

A fermentation tank having a first supply or discharge line with a first central opening arranged at the lower end of the fermentation tank for supplying or discharging a product. The fermentation tank also has a second and a third supply or discharge line, each with a centrally arranged opening for supplying or discharging a product, the three openings being arranged at different height levels. Also, a method of fermentation using the fermentation tank.