An object of the present invention is to provide a separation substrate having a high megakaryocyte blocking rate and a high platelet permeation rate, and a cell separation filter and a method for producing a platelet which use the same. The separation substrate of the present invention is a separation substrate including non-woven fabric for separating a platelet from a cell suspension containing a megakaryocyte and the platelet, in which an average pore diameter of the separation substrate is 2.0 μm to 15.0 μm, and a thickness of the separation substrate is 10 μm to 500 μm.

Systems (100) and methods for perfusion control in a bioreactor (120) are provided. The system (100) comprises a media container (110) connected to the bioreactor (120) and weighing scales (113, 123) measure weight of the bioreactor (120) and the media container (110). A plurality of controllers (225, 245) are connected to the weighing scales and configured to continuously feed the media from media container (110) to the bioreactor (120) at a user defined rate using a motor pump (112). A filter (130) is provided to receive feed from the bioreactor (120) through a recirculation line (121) and a permeate flow line (141) is connected to the filter (130) to flow out the permeate from the filter (130). When weight of the bioreactor (120) goes beyond the upper (U) or lower (L) permissible weight limit, a controller (225) connected to the weighing scale of the bioreactor (120) sends a signal to operate the permeate motor pump (142) either to flow out the permeate from the filter (130) or to stop the motor pump (142).

A bioreactor for producing hydrogen gas and other metabolites. The bioreactor utilizes light, fermentation, and other metabolic processes for the production of metabolites, derived from various microorganisms contained within the bioreactor through respective metabolic pathways. The bioreactor comprises a main reactor chamber, a semipermeable membrane, a sleeve, a power supply, a substrate medium, a heating member, a plurality of tubing members, a collection reservoir, a pressure-sealed connecter member, and an agitator.

This invention relates to compositions of matter, methods, modules and automated, end-to-end closed instruments for automated mammalian cell growth, reagent bundle creation and mammalian cell transfection followed by nucleic acid-guided nuclease editing in live mammalian cells. The disclosed compositions and method entail making “reagent bundles” comprising many (hundreds of thousands to millions) clonal copies of an editing cassette and delivering or co-localizing the reagent bundles with live mammalian cells such that the editing cassettes edit the cells and the edited cells continue to grow.

A method of obtaining a compound may include adding a substrate to a medium in a reactor, and reacting the substrate in the reactor to form the compound. A first stream is separated from the reaction liquid through a first membrane. A second stream is separated from the reaction liquid through a second membrane. The first membrane is a filtration membrane and the second membrane is configured for liquid-gas or liquid-liquid extraction The first membrane and the second membrane are at least partially immersed in the medium and are moved relative to the reactor during the separation steps.

A bioreactor (1, 2, 3) and use thereof for converting organic residual and/or waste materials into an organic nutrient solution with a proportion of at least 10% plant-available mineralized nitrogen relative to the total nitrogen content of the nutrient solution. A process for preparing an organic nutrient solution is also provided, as well as an organic nutrient solution, use of an organic nutrient solution as an absorbent for carbon dioxide storage, use of an organic nutrient solution as an agent for binding carbon in plants and soils and to a nutrient production and carbon dioxide storage system.

The purpose of the present invention is to efficiently dispense cells while reducing the risk of incorporating bacteria, etc. A cell dispensing device 1 includes: a first tube 23a which connects a container 21 and a dispensing container 22; a syringe pump 24 attached to a second tube 23b connected to a branching portion 31 formed on the first tube 23a; and a buffer tank 25 provided between the syringe pump 24 and the branching portion 31. In addition, a cell suspension contained in the container 21 is temporarily stored in the buffer tank 25 by means of the syringe pump 24, and the cell suspension stored in the buffer tank 25 is delivered to the dispensing container 22.

A yeast storage and transfer vessel having semi-permeable materials and a method for transfer of liquid yeast to a fermentor using said vessel and a peristaltic pump. In some embodiments, one or more opening(s) may include a port and valves and/or filters allowing the egress of carbon dioxide and other fermentation gases out of the yeast vessel. The bag storage vessel materials may also include semi-permeable materials. The herein disclosed apparatus and method permit the sterile transfer and mixing of yeast and/or fermentation product into a variety of fermentors, in addition to sterile pumping action in the reverse direction.

A Disposable Bioprocess System having a Single-Use-Bioreactor, a Single-Use-Pump and a single-use micro-organism retention filter. Combined most suitable for cultivation of suspended micro-organisms in a liquid media at high micro-organism concentration in a perfusion mode continuous process for expression of biological material. The inlet port of the liquid Single-Use-Pump connects via a valve to the broth reservoir of the Single-Use-Bioreactor through a liquid conveying port. The outlet port of the liquid pumping Single-Use-Pump connects via a valve to a micro-organism retention filter. And a method for operating the sterile Disposable Bioprocess System in perfusion mode for continuously processing.

A device for the decentralized and continuous production of biomass at home, which comprises: a culture container for continuous processes, in batch and in a fed batch connected to a system that provides gaseous components; and all the supplied components are sterilized by a filter; a solids dispenser that is connected to a solids reservoir, and which can quantify the dispensed solids; a dissolving-unit that is connected to the solids dispenser, to a liquids metering pump, and to a purified water generation system, and where the dissolving-unit exit is connected to the culture tank by the culture medium filters; and the latter communicate the fluids with the dissolving-unit and the culture tank; a device that contains and dispenses the inoculum that initiates the culture, and which is connected to the culture tank; a temperature-controlled rotary drum system is connected to the culture tank to separate the biomass, with a drain of liquids; a sterilizer is connected to the culture tank and the associated filters; a microprocessor with instructions to control the variables of the process, which is connected to a user interface; and connections to a water source, electric power source, drain of liquids, and to Internet and servers with AI. Procedure for the continuous production at home of biomass using said device.