The present disclosure provides a system, including methods and apparatus, for culturing, monitoring, and/or analyzing organoids. In an exemplary method of organoid culture, the method may comprise disposing a scaffold in a receptacle having an open side. A sealing member may be bonded to the open side of the receptacle to create a chamber. An organoid may be formed in the chamber using the scaffold. Fluid and/or at least one substance may be introduced into the chamber from an overlying reservoir for contact with the organoid.

The present invention relates to a gassing unit for bubble-free introduction of a process gas into a liquid located in a reactor, wherein the gassing unit comprises at least: a first gas receiving chamber and, spaced therefrom, a second gas receiving chamber for receiving a process gas, the two gas receiving chambers being connected to one another via at least two two-dimensional, gas-conducting diffusion membranes comprising hollow fibers spaced apart from one another and at least partially fixed to one another; a receptacle for a gas supply on at least one of the gas receiving chambers; a receptacle for a shaft on at least one of the gas receiving chambers;
wherein the gassing unit for gassing the liquid in the reactor can be supplied with process gas via the gas supply receptacle, can be set into a rotational movement via the receptacle for the shaft and can form a convection flow within the reactor via the rotational movement of the gassing unit in the liquid. Furthermore, the present invention relates to a method for gassing a process liquid, a gas-liquid reactor comprising a gassing unit according to the invention, and the use of a gassing unit according to the invention for supplying biological cultures with process gases.

A millifluidic device for cultures of biological agents comprising: a main body (11) comprising at least a first hole (40) closed at the bottom; a separator (35); a membrane (36) fixed to said separator (35); a plug (15) closing said first hole (40); said separator (35) designed to be placed in said first hole (40); said separator (35) being extractable from said first hole (40); said membrane (36) divides said first hole (40 ) into an upper half-chamber and a lower half-chamber; a pair of tubes (25) to perfuse said lower half-chamber; a pair of tubes (26) to perfuse said upper half-chamber; a first slide (22) placed centrally on said plug (15); a second slide (42) placed centrally on said first hole (40); a cylindrical body (41) rises from said first hole (40) and said second slide (42) is placed on the top of said cylindrical body (41); said cylindrical body (41) has a second hole (43), coaxial to said cylindrical body (41).

The present disclosure provides a bioreactor, which includes a tank configured to contain a mixture of animal cells and a liquid; and a first ventilation device arranged outside the tank and including a first gas distributor, the first gas distributor being configured to transmit a gas to the liquid separated from the animal cells. The bioreactor further includes a dialysis component, which is arranged outside the tank and comprises a dialysis filter. The bioreactor further includes a second ventilation device arranged in the tank and configured to transmit the gas to the mixture in the tank.

One aspect of the invention provides a method of generating bacteriophages adapted to infect a target bacterial strain. The method comprises: providing host bacteria that are susceptible to phage as input to a host chemostat containing phage; providing target bacteria that are related to the host bacteria, but not susceptible to phage as input to a target chemostat containing phage; filtering outflows from the host chemostat and the target chemostat to isolate phage from the populations of the host bacteria, the target bacteria, and macromolecules; combining the outflows; and introducing the combined outflow into each of the host chemostat and the target chemostat.

A bioreactor includes a culture medium vessel housing culture medium. The culture medium vessel further includes a first side surface including a first transparent optical window; and a second side surface parallel to the first surface and including one or more sensor adapters to fix optical sensors. A cell retention vessel is disposed underneath and connected to the culture medium vessel, the cell retention vessel housing biological cells and having: a top surface that intersects a base of the culture medium vessel, and a second transparent optical window indented into the top surface at a first corner of the top surface. A semipermeable membrane is disposed at a bottom of the cell retention vessel, and a frame comprising a grid is disposed underneath the semipermeable membrane.

A bioprocessing installation comprising a reservoir tank, one or more fluid lines, one or more receiving containers and an electronic control unit. The fluid line fluidically connects the reservoir tank to a receiving container. The liquid biological medium can be transferred from the reservoir tank through the fluid line into the at least one receiving container. The bioprocessing installation comprises a sensor arrangement with at least one gas bubble sensor, which sensor arrangement is configured to detect one or more gas bubbles or foam or separated phases in the liquid biological medium by a gas bubble sensor and to generate one or more corresponding sensor signals, and the electronic control unit is configured to monitor the transfer of the liquid biological medium from the reservoir tank in the direction of the at least one receiving container based on the sensor signals generated by the sensor arrangement.

Example embodiments include a cell culture apparatus, methods for cell cultivation by using the cell culture apparatus, and a cell culture incubator comprising the cell culture apparatus. The cell culture apparatus comprises a plurality of cell culture modules arranged adjacent to each other. Each cell culture module comprises two or more culture medium reservoirs connected by two or more flow channels that go through a basal chamber arranged under an apical chamber. The apical and basal chambers are separated by a porous membrane. The basal chamber has a bottom part arranged higher than a bottom part of each of the culture medium reservoirs. The culture model comprises further a cavity under at least the bottom part of the basal chamber and at least one means for capturing at least one image from a bottom and/or top of the at least one cell culture module.

One aspect of this disclosure is a method for maintaining a plankton population in a culture medium by removing particles from the culture medium. The method may comprise rotating a filter body to lift the particles from the culture medium with a filter of the filter body, positioning the filter relative to a conduit so that a first portion of the lifted particles fall into the conduit, directing a removal fluid toward the filter body to move a second portion of the lifted particles off the filter and into the conduit with impact forces applied by the removal fluid, and/or outputting an effluent flow from the conduit. The effluent flow may comprise the first and second portions of the lifted particles and a portion of the removal fluid. Aspects of related apparatus, methods, and systems also are disclosed.

The present invention relates to a hybrid system of a culture and purification process for manufacturing antibody pharmaceuticals, in which one or more devices selected from bioreactor, chromatography, and filtration equipment are provided with one or more materials selected from among SU disposable bags and stainless steel (SS), and one or more devices selected from preparation and storage tanks of a medium and a buffer are provided with SS material, thereby simultaneously obtaining the advantages of a SS process system and a SU process system, and preventing cross-contamination through independent operation of a culture unit and a purification unit, and a process system is designed and positioned such that an effluent stream exiting individual culture units is introduced into one or more purification units using a control unit, thereby avoiding a bottleneck phenomenon from occurring in the purification process.