The invention discloses a first unit (1) for treatment of a bioprocess liquid comprising a first lateral face (2), a second lateral face (3) and a front face (4) which meets the two said lateral faces. The front face comprises: a plurality of valves (7) adapted to receive and act upon one or more legs (8) of a disposable flow path (6); optionally one or more pumps (10) adapted to receive and act upon one or more legs of the disposable flow path; optionally one or more sensors (11) adapted to receive and to measure one or more parameters in one or more legs of the disposable flow path; wherein the plurality of valves and optional pumps and sensors are vertically offset from each other to give one or more legs of a disposable flow path received by said valves and optional pumps and sensors a slope of at least 3.0 degrees from the horizontal plane (h).

One or more embodiments of the present disclosure relate to a culture device, which comprises: an accommodation base, an accommodation chamber disposed on the accommodation base. The accommodation chamber includes a culture chamber for accommodating one or more cultures. The culture chamber includes a chamber bottom wall and a chamber side wall surrounding the chamber bottom wall. A culture solution channel is formed between an outer side of the chamber side wall and an inner wall of the accommodation chamber. At least part of the chamber side wall includes a membrane material, and a plane where the membrane material is located intersect with the chamber bottom wall.

Embodiments described herein generally provide for expanding cells in a cell expansion system. The cells may be grown in a bioreactor, and the cells may be activated by an activator (e.g., a soluble activator complex). Nutrient and gas exchange capabilities of a closed, automated cell expansion system may allow cells to be seeded at reduced cell seeding densities, for example. Parameters of the cell growth environment may be manipulated to load the cells into a particular position in the bioreactor for the efficient exchange of nutrients and gases. System parameters may be adjusted to shear any cell colonies that may form during the expansion phase. Metabolic concentrations may be controlled to improve cell growth and viability. Cell residence in the bioreactor may be controlled. In embodiments, the cells may include T cells. In further embodiments, the cells may include T cell subpopulations, including regulatory T cells (Tregs), helper, naïve, memory, or effector, for example.

Provided is a cell suspension culturing apparatus suitable for large-scale culture of cells, the cell culturing apparatus comprising a culture solution aspirator having a double-tube structure comprising an outer tube and an inner tube inserted in a lumen of the outer tube, and a suspension culture vessel, wherein in the culture solution aspirator, the outer tube comprises a filter through which a culture solution is passed, the inner tube comprises a suction port for the culture solution passed through the filter, and an air hole which communicates the lumen of the outer tube with the outside is disposed distally in a direction of insertion of the outer tube into the suspension culture vessel.

Various embodiments of the present technology provide methods and systems for soil enrichment. The systems may comprise a bioreactor system coupled to an initial treatment system for the cultivation of a live microorganism culture containing organic nutrients on an agriculturally effective scale. The systems may be automated and/or portable for practical applications onto target fields. The live microorganism culture may be delivered onto the soil of the target fields, enriching the soil with the organic nutrients that become bioavailable to crops growing in the soil. The soil enrichment system may provide a sustainable approach to agriculture that may efficiently enhance the natural processes of the native soil of any crop.

The present disclosure relates to a new use of a tangential flow filtration device and a continuous concentration system for producing an ultra-high concentration medical exosome solution.

The present invention is a manufacturing method for producing fermentation products using a fermentation vessel, including steps of: preparing a fermentation vessel and a sensor, introducing liquid into the fermentation vessel, and operating the fermentation vessel in which properties of liquid in the fermentation vessel are measured to adjust operating conditions; wherein the sensor device has a sensor for measuring liquid properties and a sensor cover body; a bottom permeable portion for passing liquid and crystals in the liquid is disposed on the bottom surface of the cover body, and a top permeable portion for passing liquid and crystals in the liquid is disposed on the top surface of the cover body; micropores are respectively formed in the bottom permeable portion and the top permeable portion; and micropores disposed in the top permeable portion are the same or larger than micropores disposed in the top permeable portion.

An anaerobic digestion device based on self-sustained air flotation includes an anaerobic digestion tank unit, a self-sustained air flotation screening unit and a biogas measurement and collection unit. The self-sustained air flotation screening unit includes an air flotation screening part, a material sedimentation part, a reflux part and a three-phase separation part connected sequentially from bottom to top. A digested material in the anaerobic digestion tank unit is pumped into the air flotation screening part, overflows into the material sedimentation part, and then is raised to the reflux part. Gas passing through the three-phase separation part and gas produced in the anaerobic digestion tank unit enter the biogas measurement and collection unit to be measured and collected.

The present invention relates to a Disposable Bioprocess System consisting of a Single-Use-Bioreactor, a Single-Use-Pump and a single-use micro-organism retention filter. Combined most suitable for cultivation of suspended micro-organisms in a liquid media at high micro-organism concentration in a perfusion mode continuous process for expression of biological material. The inlet port of the liquid Single-Use-Pump connects via a valve to the broth reservoir of the Single-Use-Bioreactor through a liquid conveying port. The outlet port of the liquid pumping Single-Use-Pump connects via a valve to a micro-organism retention filter. And a method for operating said sterile Disposable Bioprocess System in perfusion mode for continuously processing.

An improved dry grind system and method for producing a sugar stream from grains or similar carbohydrate sources and/or residues, such as for biofuel production. In particular, a sugar/carbohydrate stream, which includes a desired Dextrose Equivalent (DE) where DE describes the degree of conversion of starch to dextrose (aka glucose) and/or has had removed therefrom an undesirable amount of unfermentable components, can be produced after saccharification and prior to fermentation (or other sugar conversion process), with such sugar stream being available for biofuel production, e.g., alcohol production, or other processes. In addition, the systems and methods also can involve the removal of certain grain components, e.g., corn kernel components, including protein, oil and/or fiber, prior to fermentation or other conversion systems. In other words, sugar stream production and/or grain component separation occurs on the front end of the system and method.