A system for performing a gas-liquid mass transfer includes a container bounding a compartment and having a top wall, a bottom wall, and an encircling sidewall extending therebetween. A tube has a first end and an opposing second end, the first end of the tube being disposed within the compartment of the container. A nozzle is disposed within the compartment of the container and has at least one outlet, the nozzle being coupled with the tube so that a gas can be passed through the tube and out the at least one outlet of the nozzle. The nozzle is sufficiently buoyant so that when a fluid is disposed within the compartment of the container, the nozzle floats on the fluid.

A condenser bag includes a body bounding a channel that extends between an inlet opening at a first end and an outlet opening at an opposing second end, the body being comprised of a polymeric film; an intake port bounding a port opening that extends therethrough, the intake port being directly physically secured to the first end of the body so that the port opening communicates with the channel of the body; and a tubular transfer line having a first end directly mechanically coupled with the body at a first location located between the inlet opening and the outlet opening so as to communicate with the channel and an opposing second end directly mechanically coupled to the intake port so as to communicate with the port opening thereof.

The present invention pertains to methods for manufacturing high titer antibody products. In particular, the invention pertains, in part, to improved serum-free animal cell culture medium, which can used for the production of a protein of interest. Additionally, the present invention further pertains to chromatographic procedures employed to successfully isolate the antibody product subject of the present disclosure.

Miniature stirred tank bioreactor and system; the bioreactor configured for single use culturing providing unobstructed culturing volume with sensors, probes and ports using minimally invasive placement in-wall or across the wall methods; the bioreactor further capable of containing scaffolds and features for culturing cells and tissue, including structures providing surfaces for cell growth, and screens for retaining microparticles during media exchange.

An apparatus for managing gas bubbles in a bioprocessing system includes a body portion having a underside surface, and an opening in the body portion. The body portion is configured for placement within a bioreactor vessel such that the underside surface of the body portion is disposed in a liquid within the bioreactor vessel. The underside surface of the body portion is configured to divert rising gas bubbles in the liquid towards the opening.

The present invention pertains to methods for manufacturing high titer antibody products. In particular, the invention pertains, in part, to improved serum-free animal cell culture medium, which can used for the production of a protein of interest. Additionally, the present invention further pertains to chromatographic procedures employed to successfully isolate the antibody product subject of the present disclosure.

A method for filtering a gas includes delivering a gas into a compartment of a gas filter assembly; applying a partial vacuum to the gas filter assembly so that the partial vacuum assists in drawing the gas through a porous filter body of the gas filter assembly that is at least partially disposed within the compartment of the gas filter assembly; and regulating the application of the partial vacuum based on a pressure reading of the gas upstream or downstream of the gas filter assembly.

A mixed gas generated in a methane fermentation tank is supplied to culture tanks. In the culture tanks, water as a culture solution is held and algae are cultured. Carbon dioxide contained in the mixed gas preferentially dissolves in water. That is, carbon dioxide is removed from the mixed gas. As a result, a concentration of methane, which is one component of the mixed gas, increases. The mixed gas is re-supplied to another culture tank or the same culture tank.

An assembly for delivering a fluid includes a fluid dispenser connected to a fluid supply conduit. The fluid dispenser includes a fluid outlet positioned along a length of the fluid dispenser. The fluid outlet is configured to deliver the fluid from the fluid supply conduit at a flow rate that varies along the length of the fluid dispenser.

A cell culture system for culturing cells is provided. The cell culture system including a cell culture vessel with a cell culture chamber for culturing cells, an inlet through which liquid can flow for filling the cell culture vessel, and an outlet through which fluid can exit the cell culture vessel. The system also includes at least one fill sensor arranged to detect liquid within the cell culture vessel reaching a fill level at a predetermined position of the cell culture vessel during filling of the cell culture vessel, where the at least one fill sensor can generate a detection signal when liquid within the cell culture vessel reaches the fill level. Also included is an actuator to change an orientation of the cell culture vessel in response to the detection signal.