Systems and methods relating to dynamic spargers for generating fine bubbles within reactors such as biological and chemical reactors. A sparger system is positioned within a reactor and comprises a support plate, multiple annular shrouds engaged with the support plate, and spargers positioned within the annular shrouds defining a gap between an interior surface of the annular shroud and an exterior surface of the corresponding sparger. Liquid flows through the defined gap between an interior surface of the annular shroud and an exterior surface of the sparger. Acceleration of the liquid through the gap shears bubbles at the exterior surface of the sparger creating bubbles or fine bubbles.

Embodiments of the present invention provide an automatic micro algae culturing device and system for self-cleaning, continual automatic algae culturing and irradiant optimizing. The culturing device includes a photosynthesis tubular reactor for micro algae culturing, with transparent cleaning particles to scrape off any unwanted particles or components such as including and not limited to formation of biofilm. The device also includes a nano-air bubble generating device installed around the tubular reactor to generate and feed nano-air bubbles into the tubular reactor, where the nano-air bubbles increase an active surface area for ions to interact with the algae in the culture medium in order to increase productivity of the culturing device by enhancing rate of mitosis, productively of oil, protein and polysaccharide and the nano-air bubbles also sterilizes the culture medium.

The systems and methods disclosed herein provide for the efficient generation of fine bubbles. In particular, systems and methods for use in bioreactors are disclosed herein providing a superior means to produce useful fermentation products by the biological fermentation of fine bubble waste substrates injected into a liquid broth containing a microorganism culture.

A bioreactor configured to contain a volume of liquid is provided. The bioreactor includes a collapsible bag able to contain the volume of liquid, a first sparger connected to the collapsible bag, and a second sparger connected to the collapsible bag.

Provided herein are a device and a method for preparation of immobilized proteins, enzymes or cells on a carrier to achieve the industrial batch production of the immobilized proteins, enzymes or cells.

A culture vessel that can suppress foaming in a culture medium when a common nozzle is used to supply a liquid culture medium and a gas together. This culture vessel includes: a vessel body having an opening portion that communicates with a housing space; a lid that closes the opening portion; and a nozzle that passes through the and extends to the housing space, and supplies a liquid culture medium and a gas together. The nozzle has a vent opening portion that is formed so as to open in a side of the nozzle and serves as a movement path of the gas from inside of the nozzle into the housing space.

The method of growing a biopolymer material employs incubation of a growth media comprised of nutritive substrate and a fungus in containers that are placed in a closed incubation chamber with air flows passed over each container while the chamber is maintained with a predetermined environment of humidity, temperature, carbon dioxide and oxygen. The air flows may be directed parallel or perpendicularly to the surfaces of the growth media.

A cell culture device includes a control unit that includes a first gas supply source, a second gas supply source, and a decompression source, and a culture unit that includes a first container, a second container, a first gas channel that connects a third container, the first gas supply source, the second container to a connection point, a second gas channel, a third gas channel, a first liquid channel, a second liquid channel, a gas-liquid channel, a first valve, a second valve, a third valve, a fourth valve, and a fifth valve.

The present disclosure provides an in vitro life culture system, comprising: a culture module used to cultivate a culture, the culture module including at least a culture chamber for holding a culture fluid; a culture fluid provision module used to provide the culture fluid to the culture module; and a fluid output module used to discharge the culture fluid from the culture chamber.

A calculation device (10) includes a calculation unit (14) that: acquires culture data over time indicating a relationship between a DO value detected in a culture solution and an oxygen supply amount controlled by a freely-selected control method, the culture solution being stored in a first culture tank (2) in which a culturing target material is cultured; identifies a culture model indicating a relationship between the oxygen supply amount for the culturing target material increasing over time and the DO value, based on the culture data; and executes simulation of virtually culturing the culturing target material in a second culture tank (20) using a culture control method of culturing the culturing target material based on the culture model and the oxygen supply amount in the second culture tank, and calculates parameters to be used in the culture control method based on the simulation result.