The present invention relates to a fermentation process for the fermentation of at least one microorganism, wherein the fermentation process comprises the steps of (a) allowing a fermentation broth comprising the at least one microorganism to flow in the fermentation reactor; (b) supplying a carbon-substrate to the fermentation reactor allowing the gaseous carbon-substrate to be dissolved, or partly dissolved, in the fermentation broth; (c) supplying a nitrogen-substrate to the fermentation reactor allowing the gaseous nitrogen-substrate to be dissolved, or partly dissolved, in the fermentation broth; and (d) maintaining a nitrate concentration of the fermentation broth below 0.035 g/l, and/or maintaining a nitrate concentration of the fermentation broth below 0.01 g nitrate/g biomass; wherein the at least one methanotrophic organism comprises at least one methanotrophic microorganism.

Provided is a culture device, including: a culture tank (110) configured to store an object liquid that is a culture solution having suspended therein an object; a screen (210) made of a metal, the screen including a main body (212) and a plurality of through holes (214) passing through the main body from a front surface (212a) thereof to a back surface (212b) thereof; a spray portion (220) configured to spray the object liquid stored in the culture tank onto the front surface of the body; an accommodation portion (240) surrounding the back surface of the main body and configured to accommodate the culture solution having passed through the through holes; a UV light irradiation portion (250) arranged in the accommodation portion and configured to radiate UV light; and a return portion (290) configured to return the culture solution in the accommodation portion to the culture tank.

Embodiments herein are directed to methods of heterotrophically culturing a. Embodiments herein are directed to methods, systems, and bioreactors for heterotrophically culturing Euglena sp. microorganism, a Schizochytrium sp. microorganism, or a Chlorella sp. microorganism comprising: culturing the microorganism in a culture media containing one or more carbon source, one or more nitrogen source, and one or more salt; maintaining a pH of between about 2.0 to about 4.0; maintaining a temperature of about 20° C. to about 30° C.; and maintaining an environment with substantially no light; wherein the culturing occurs in three cultivation stages.

The present set of embodiments relate to a system, method, and apparatus for hydrating and mixing a liquid medium from a dry medium. Such systems, methods, and apparatuses can be used in the biotech industry because they can be used to provide prepackaged dry media in a format that can be quickly rehydrated free of issues with operator error found in more conventional media rehydration systems using mixing tanks and reactors.

A bioreactor support system configured to hold a bioreactor bag, said bioreactor support system (1) comprising: —a base module (3) comprising an impeller drive unit (5); and —at least two vessel units (11a, 11b, 11c) of different sizes, which are configured to support and substantially enclose a side wall of a bioreactor bag (111a, 111b, 111c) when a bioreactor bag is provided in the bioreactor support system (1), wherein said base module (3) and said at least two vessel units (11a, 11b, 11c) comprise mating connection devices (13a, 13b, 13c, 15a, 15b, 15c) such that the at least two vessel units (11a, 11b, 11c) can be connected to one and the same base module (3) and such that the impeller drive unit (5) of the base module (3) can be used to drive an impeller (113a, 113b, 113c) in a bioreactor bag (111a, 111b, 111c) when a bioreactor bag is provided in the bioreactor support system (1).

A large amount of three-dimensional cells are efficiently produced. To this end, a cell is cultured by using a culture medium containing an ultra-fine bubble generated by heating a heating element to make film boiling on an interface between a liquid W and the heating element.

A system for growing algae includes a gas concentrator configured to receive a first gas containing CO2 at a first concentration and discharge a second gas containing CO2 at a second concentration higher than the first concentration, and a photobioreactor containing algae in an algae slurry and fluidically coupled to the gas concentrator to receive the second gas. A recycling line extends from a gas outlet on the photobioreactor to remove a third gas from the photobioreactor for future use in the photobioreactor.

The inventions described herein concern improved bioreactors and methods of aerating fluids.

Embodiments of the present invention provide an automatic micro algae culturing device and system for self-cleaning, continual automatic algae culturing and irradiant optimizing. The culturing device includes a photosynthesis tubular reactor for micro algae culturing, with transparent cleaning particles to scrape off any unwanted particles or components such as including and not limited to formation of biofilm. The tubular reactor has multiple double-walled glasses. Inner walls of the tubes in the tubular reactor may be coated with superhydrophobic coating to avoid bio film formation, or sticking of any hydro dirt or dust particles. Because the cleaning particles are transparent, they wont block any sunlight for photosynthesis in the tubular reactor.

The apparatuses described herein relate to preparation of a meat product. Apparatuses, systems comprising the apparatuses, and methods of making and use the systems and apparatuses are described herein. These are useful for controlling one or more of growth on and separation of a meat product from an enclosed substrate. The apparatuses and systems are configured to receive fluid and grow the meat product and/or separate the meat product from the substrate in a scalable manner.