A methane generating device includes: a culture tank in which methane generating bacteria are cultured with a culture liquid; a gas feed device that feeds carbon dioxide and hydrogen gas required by the methane generating bacteria to the culture tank; and a generated gas extraction device that extracts methane generated by the methane generating bacteria.

A cell and tissue sheet forming package includes a container body, a membrane, a sliding door plate and a sealing film. The sliding door plate is disposed slidably on a top of the container body to cover or expose the membrane. The sliding door plate has a hole and a passive magnetic assembly. The cell injection equipment includes a carrier, an injection mechanism and a drive mechanism. The carrier carries the package, and the drive mechanism moves the carrier and the injection mechanism to have the injection mechanism to inject a solution, through the hole, into the package. A heating element of the carrier is introduced to heat the membrane and the solution to transform the solution into a colloid sheet on the membrane. Then, the positive magnetic assembly engages magnetically the passive magnetic assembly to slide the sliding door plate to expose the colloid sheet on the membrane.

A method for filtering a gas includes delivering a gas into a compartment of a gas filter assembly; applying a partial vacuum to the gas filter assembly so that the partial vacuum assists in drawing the gas through a porous filter body of the gas filter assembly that is at least partially disposed within the compartment of the gas filter assembly; and regulating the application of the partial vacuum based on a pressure reading of the gas upstream or downstream of the gas filter assembly.

A fluid transfer assembly for single use bioreactors includes a fluid transfer housing that can be mounted to the impeller shaft using a bearing that places the fluid transfer assembly directly below the lowest impeller but allows the impeller shaft to spin inside independently of the fluid transfer assembly. A fluid conduit connects the fluid transfer housing to a port in the single use bag wall which allows fluids to be introduced into the sparger and which also helps prevent the fluid transfer assembly from rotating with the impeller shaft.

Provided is a high throughput photobioreactor. The high throughput photobioreactor includes: a chamber; a plate installed in the chamber and mounted with a plurality of wells; a plurality of light sources installed in the chamber and irradiating light toward the plate; a light quantity controller positioned on an upper part of the plate to make quantities of light irradiated to the plurality of wells different; and a temperature controller controlling a temperature of the plate.

The present invention is a composting bioreactor system that continually receives biodegradable solid wastes, waste waters and exhaust gases, automatically recycles the biodegradable wastes into nutrients and heat energy, and automatically supplies the nutrients and heat into an integrated hydroponic or aquaponic system. This invention together with integrated food growing system may be installed onsite such as balconies, backyards and premises of restaurants and food factories etc. therefore may lead to zero mileage targets both for recycling wastes and for supplying foods. This invention integrates composting process and aquaponic technology together and may establish a closed-loop recirculation of both water and gases therefore upgrades aquaponics into compoponics. A compoponic system has both soil and soilless growing beds and mimics nature recirculating nutrients, carbon and energy among human being, animals, microorganisms and plants by way of photosynthesis, slow burning by cellular respiration and burning by combustion.

A single-use bioreactor is provided. The single-use bioreactor may include a bioprocess container, a shell, at least one agitator, at least one sparger, at least one gas filter inlet port for the sparger(s) and headspace overlay, at least one fill port, at least one harvest port, at least one sample port, and at least one probe. In examples, at least one controller may monitor and control one or more parameters associated with the single-use bioreactor A method to cultivate and propagate mammalian cells is also provided. The method may include cultivating under suitable conditions and in a suitable culture medium in a first single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a second single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a third single-use bioreactor, and cultivating the cells in the third bioreactor.

A method and system for producing hawthorn fermented beverage where hawthorn fruits are used as the main material to produce a hawthorn fermented beverage with special fermented flavor, rich nutrition, mellow mouth feel and suitable sourness and sweetness, through the processes of fruit sorting, cleaning, juice squeezing, blending, pre-sterilization, alcoholic fermentation, acetic fermentation, lactic acid fermentation, filtration, sterilization and filling. An atomized fermentation technology is adopted for the yeast aerobic proliferation during the alcoholic fermentation phase and for the acetic fermentation phase, so that, as compared with the liquid submerged fermentation methods, the fermentation time of these two stages are shortened by 10-15 hours and 62-100 hours respectively, and the fermentation period is shortened by 3-5 days, which improves the production efficiency of fermentation.

A method for displacing a fluid and simultaneously gas enriching a liquid cell culture medium with a gas. The method includes injecting a controlled volume of a gas or gas mixture into a one chamber by using a gas flow controller, the injection taking place through a gas inlet into a volume of liquid. This injection produces bubbling and agitation of the volume of liquid; a build-up of gas or gas mixture due to buoyancy in a hermetic space formed by the volume of liquid and the chamber, and a pressure increase in the chamber until a sufficient controlled pressure is reached of less than or equal to 10 bar. This increase displaces the volume of liquid by a fluid outlet connecting the volume of liquid to the exterior of the chamber. Also provided are a device implementing the method and a cell culture system in a multi-well culture plate.

An aerosolization device for an aerosol wetting chamber comprising a receiving opening for receiving the aerosolization device and adapted for wetting samples disposed in the aerosol wetting chamber with aerosol, the aerosolization device comprising a main body, an aerosol deflector and a swirling device.