A process for reducing the production of sludge by municipal or industrial wastewater purification plants, comprising a step of mesophilic or thermophilic anaerobic digestion (20), or anaerobic digestion combining these two operating modes, of a stream of sludge to be treated (1), and at least one biological solubilization anaerobic treatment step (30); the process comprises, upstream of the anaerobic digestion step, a step of dehydration (10) of the sludge to be treated, followed by a step of mixing (15) the dehydrated sludge with a recirculated fraction of sludge that is more liquid, originating from recycling of the digestion (20), and/or from the anaerobic treatment step (30), and/or centrates originating from a final dehydration (50) of the treated sludge, wherein the recirculation rate is chosen such that the mixture has a dryness suitable for digestion, this mixture then being directed towards the digestion.

A sparger assembly for a bioprocessing system includes a first layer having a plurality of pores of a first size, and a second layer disposed above the first layer and having a plurality of holes of a second size, the second size being greater than the first size. The pores of the first layer and the holes of the second layer allow for the passage of a sparge gas through the first layer and the second layer.

The apparatuses described herein relate to preparation of a meat product. Apparatuses, systems comprising the apparatuses, and methods of making and use the systems and apparatuses are described herein. These are useful for controlling one or more of growth on and separation of a meat product from an enclosed substrate. The apparatuses and systems are configured to receive fluid and grow the meat product and/or separate the meat product from the substrate in a scalable manner.

Some embodiments include a microalgae culturing system including a bioreactor adapted to propagate microalgae in a culture solution using in combination at least one of natural and artificial light, and at least one nutrient including at least a carbon source, where the microalgae are freely suspended in and form part of the culture solution. A microalgae feed source is coupled to the bioreactor and a first controller between a water conditioning assembly and the bioreactor. The water conditioning assembly is coupled as an input of supply water to the bioreactor, and configured to condition the supply water to a specified purity that enables substantially unhindered growth of the microalgae in the culture solution to a specified concentration, and the first controller is configured to control supply of the microalgae feed source to the bioreactor.

A crossflow microfluidic particle concentrator including a main channel having an inlet and an outlet a crossflow outlet operably connectable with pressure means and/or flow control means, a plurality of crossflow channels fluidically connecting the crossflow outlet with the main channel a filtering element including a particle flow channel within the main channel, and a row of crossflow pillars disposed between the filtering element and the plurality of crossflow channels.

A culture medium replacement device includes: multiple reservoirs that are mounted on a well plate including multiple wells, which each contain a culture and a culture medium, and are provided respectively for the multiple wells and that each contain a culture medium to be supplied to a corresponding well; a gas supply pump that discharges a gas supplied to the multiple reservoirs; a gas supply passage unit that connects the gas supply pump and the multiple reservoirs and that transmits the gas from the gas supply pump to the multiple reservoirs; and multiple liquid supply passages that each connect a reservoir and a corresponding well and transmit, to the corresponding well, the culture medium pushed out from the reservoir by the gas flowing into the reservoir.

A cell culture bioreactor has perfusion membranes and gas transfer membranes or a gas phase in an extra-membrane space in contact with a film on the perfusion membranes. Gas transfer membranes may travel through the perfusion membranes or through the extra-membrane space. Examples with hollow fiber and flat sheet membranes are shown. One or more of the membranes optionally has a responsive surface, for example a thermo-responsive surface. In some examples, membranes are located in X-Y planes while the length of the reactor extends in a Z-direction.

The disclosure relates to methods of capturing carbon by microbial fermentation of a gaseous substrate comprising CO into one or more first products which, in turn, may be incorporated into an article of manufacture or one or more second products. Further, the disclosure relates to improving carbon capture and/or efficiency.

CO.sub.2, and other gases are utilized with mineral feedstock to synthesize products. The synthesized products, as the result of liquid, solid, gas photo-chemical reactions within the advanced bioreactor of the disclosed embodiment, are precipitated raw material for multiple consumer and industrial products. Waste heat, pressure and torque produced from the bioreactor are utilized for generating electricity and/or heat through a combination of energy recovery devices. Energy recovery devices offsets and lower the cost of operating the reactor as the disclosed reactor integrates photolysis via ultra-violet light, as an integral component, of a reactor system, composed also of an active mixer-agitator assembly, pressure and vacuum vessel chamber, heat source, and ports for media ingestion. The disclosed reactor is designed be conducive to transforming gaseous, solid, and liquid feedstock, like carbon dioxide —CO.sub.2, and other feedstocks that are inorganic and/or organic in an aqueous medium, into inorganic and organic products.

The invention relates to the use of microjet reactor for processing biomass. The cell lysis of flowable biomass is thereby carried out by means of multiple high-speed liquid jets which collide with one another, wherein the liquid jets contain the cells or consist wholly of the flowable cell mass, wherein intact or wholly or partially lysed biomass is added to at least one of the colliding high-speed liquid jets, and an extraction takes place simultaneously with the collision of the liquid jets or subsequently thereto. The lysis of the cells is initiated or facilitated by the forces that occur on acceleration, introduction of the acceleration, collision of the jets and mixing of the jet constituents.