An object of the present invention is to provide an artificial tissue perfusion device capable of analyzing the interaction between a vascular cell layer and a parenchymal cell layer with high accuracy. An artificial tissue perfusion device includes a co-culture system (C) in which a plurality of types of cell are cultured. The co-culture system has a tubular well part (10) having a culture space (11) inside; a base material (20) having a perfusion flow path (26) which extends in a predetermined direction and is perfused with a medium, and a holding part (23) which opens to the perfusion flow path and holds the well part attachably and detachably; and a gel membrane (30) having a form of a porous membrane and disposed at an end portion of the well part facing the perfusion flow path in a case where the well part is held by the holding part. A tissue-based cell is cultured on a surface side of the gel membrane facing the culture space, and a luminal cell is cultured on a surface side of the gel membrane facing the perfusion flow path.

A bioreactor comprising a housing defining a perfusion chamber, the housing including at least one port, wherein the at least one port is coupled to the housing, and a sample holder positioned within the perfusion chamber. A bioreactor and spheroid-based biofabrication method for making perfusible tissue constructs and perfusing them.

A packed-bed bioreactor system for culturing cells is provided, the system including a cell culture vessel having at least one interior reservoir, an inlet fluidly connected to the reservoir, and an outlet fluidly connected to the reservoir; and a cell culture matrix disposed in the reservoir. The cell culture matrix includes a structurally defined multi-layered substrate for adhering cells thereto, and each layer of the multi-layered substrate has a physical structure and a porosity that are substantially regular and uniform.

Disclosed is a microfluidic device having a channel a chamber in fluid communication with the channel. The chamber is sized to contain dental sample that is form a semipermeable barrier through which fluid from the channel is deliverable to the dental sample.

A fixed-bed bioreactor system is provided that includes a vessel with a media inlet, a media outlet, and an interior cavity disposed between and in fluid communication with the media inlet and media outlet. The vessel further includes a cell culture substrate disposed in the interior cavity between the media inlet and the media outlet in a packed-bed configuration, the cell culture substrate including a plurality of porous disks in a stacked arrangement. The interior cavity includes a cell culture section and a spacer section, the cell culture substrate defining the cell culture section and the spacer section being disposed between the cell culture section and the media outlet, and each of the plurality of porous disks has a surface configured to culture cells thereon.

Filter elements for perfusion systems and methods are provided. A filter element sheet includes a microporous membrane having a mean pore size of at least about 0.65 μm and a feed spacer comprising woven fibers and having an open area of at least about 35%. The filter element sheet can be arranged within a filter element, for example, in a spiral-wound format or in a cassette format. A perfusion system includes at least one filter element and a pump configured to control flow of a liquid feed through the at least one filter element.

A multi-layer microfluidic bioreactor is used in methods for culturing cells. A grooved semipermeable substrate can capture and grow cells using perfusion culture methods. Microfluidic geometry directs flow over grooves for cell expansion and along grooves for cell harvesting. The bioreactor and methods can be optimized for high density and automated processing for growth of cells.

The disclosure relates generally to a cell culture apparatus and a cell culture method. One aspect of the disclosure is an oxygen-permeable bag comprising one or more polymer films defining a boundary of an interior compartment of the bag, each of the one or more polymer films comprising an inner layer adjacent the interior compartment of the bag, the inner layer comprising a fluoropolymer, and adhered to the inner layer, an outer layer comprising polymer; a first port formed in an exterior surface of the bag and in fluid communication with the interior compartment; a second port formed in an exterior surface of the bag and in fluid communication with the interior compartment; and contained in the interior compartment, a plurality of microcarriers.

Described herein is a modular continuous flow bioreactor for various cells. In one embodiment, the modular cell culture bioreactor apparatus may comprise a plurality of cell chambers disposed between an upper flow chamber and a lower flow chamber; a plurality of lower conduits fluidly connecting the lower flow chamber with one or more lower reservoirs and a plurality of upper conduits fluidly connecting the upper flow chamber with one or more upper reservoirs; one or more pumps fluidly connected through the plurality of conduits with the one or more reservoirs and with the upper and lower flow chambers; wherein each individual cell chamber comprises a lower permeable membrane in fluid communication with the lower flow chamber, a three-dimensional distribution of cells, and an upper permeable membrane in fluid communication with the upper flow chamber.

The present invention provides a microfluidic devices and methods of use thereof for the concentration and capture of cells. A pulsed non Faradaic electric field is applied relative to a sample under laminar flow, which results to the concentration and capture of charged analyte. Advantageously, pulse timing is selected to avoid problems associated with ionic screening within the channel. At least one of the electrodes within the channel is coated with an insulating layer to prevent a Faradaic current from flowing in the channel. Under pulsed application of a unipolar voltage to the electrodes, charged analyte within the sample is moved towards one of the electrodes via a transient electrophoretic force.