The present invention relates to a method for cultivating cells, in particular tissues, comprising a carrier plate unit, which has at least one access opening, at least one cultivation chamber, and at least one channel connecting the access opening to the cultivation chamber.

A pulsatile perfusion bioreactor for culturing one or more engineered blood vessels having a lumen and a wall is provided. The bioreactor includes a chamber for holding the engineered blood vessel and cell culture media; a mechanical property monitoring system for measuring axial tensile stress and strain, circumferential tensile stress and strain, and/or shear stress imparted on the vessel wall; and a pump system for delivering cell culture media through the vessel lumen, wherein the vessel is exposed to a composite pressure waveform and a composite flow waveform as the media is delivered there through. The pump system includes a steady flow and peristaltic pumps. Further, the composite pressure and flow waveforms each include a mean component, a fundamental frequency component, and a second harmonic frequency component. The bioreactor also includes a computer interface for monitoring and adjusting the composite waveforms to maintain a predetermined stress levels.

The invention relates to a microbioreactor module for the three-dimensional cultivation of cells, especially stem cells. Said microbioreactor is intended for single use and, in an embodiment of the invention, can be used as a multi-microbioreactor.

An invention proposes a photobioreactor with a cultivation chamber in the form of a shallow closed duct that is irradiated by the sun light. The duct is constructed from an inflatable sleeve from transparent polymer film; this shallow duct is sandwiched between a bank of frames with wire nettings from below and glass panes from above, which are secured on the shelves of the longitudinal sides of the frames.

The shallow duct has small inclination regarding the horizontal plane and the length until several hundred meters. Two headers are supplying and removal of gaseous medium and suspension of microalgae into and out of the shallow duct.

A system and method for automated and closed cell culture system. The system includes a disposable assembly, actuators, sensors, software/firmware and smart device App. The disposable includes fresh media, drug or reagents containers, waste container, sample out containers, cell culture flask, well, bag or bioreactor and active or passive pumping elements. The system is expanded to multiple containers cell or organ processing. In all these precisely controlling the fluids are achieved by interdigitated differential capacitance measurements. The system has provision for imaging, machine vision control, controlled over the internet, volume metering and can use wide variety of pumps for actuation. The system is programmed to perform cGMP protocols and operated as a portable instrument.

A bioartificial liver device including a bioreaction chamber having a plurality of semi-permeable membranes and a plurality of filter spaces each confined by two adjacent semi-permeable membranes; a plurality of liver cell perfusion ports each communicating with one of the filter spaces for introducing the liver cells into the filter spaces, and a positive peristaltic pump. In the device, the semi-permeable membranes are disposed substantially horizontal with respect to the ground, and the positive peristaltic pump is adapted to drive the plasma flow from the bottom wall of the device to the top wall. The device of the invention improves the cell loading and the area for substance exchange between the blood and the liver cells.

Described are systems, methods, and devices relating to normothermic extracorporeal support of an organ, tissue, or bioengineered graft comprising cross-circulation (XC) perfusion for prolonged periods (days to weeks) via an XC perfusion circuit in connection with an extracorporeal host (e.g., animal, patient, organ transplant recipient) are disclosed. The XC perfusion circuit comprises auto-regulation of blood flow based on the trans-organ blood pressure difference between arterial and venous pressure. Recipient support enabled 36 h of normothermic perfusion that maintained healthy lungs with no significant changes in physiologic parameters and allowed for the recovery of injured lungs. Extended support enabled multiscale therapeutic interventions in all extracorporeal lungs. Lungs exceeded transplantation criteria.

A system and method for automated and closed cell culture system. The system includes a disposable assembly, actuators, sensors, software/firmware and smart device App. The disposable includes fresh media, drug or reagents containers, waste container, sample out containers, cell culture flask, well, bag or bioreactor and active or passive pumping elements. The system is expanded to multiple containers cell or organ processing. In all these precisely controlling the fluids are achieved by interdigitated differential capacitance measurements. The system has provision for imaging, machine vision control, controlled over the internet, volume metering and can use wide variety of pumps for actuation. The system is programmed to perform cGMP protocols and operated as a portable instrument.

The present invention provides a cell cultivation device comprising a polymer porous film, and a cell cultivation method using said cell cultivation device. Provided is a cell cultivation device that has a siphon structure or a shishi-odoshi structure, said cell cultivation device comprising: a polymer porous film; a cell cultivation part that accommodates the polymer porous film; a liquid reservoir part that has the cell cultivation part stored in an interior thereof; a culture medium supply means that is positioned at an upper section of the liquid reservoir part; a culture medium discharge means that is positioned at a lower section of the liquid reservoir part; and a culture medium recovery means that recovers the culture medium that is collected in the liquid reservoir part and is intermittently expelled.

Disclosed herein are multi-cuvette cartridges, flow cells, and electrical pulse systems for treatment of large volumes of biological samples with pulsed electric fields. The multi-cuvette cartridges systems may include mechanical motors for automatic positioning and alignment between the cuvettes and the electrodes of the electrical pulse systems. The flow cell systems may include fluidic systems, such as pumps, nozzles and valves, which may operate in coordination with the electrical systems for efficient exposition of biological samples. Embodiments having flow cell systems that allow on-demand production of activated sample are also disclosed.