A continuous process for the synthesis of biodiesel from microalgae oil by transesterification with supercritical methanol, where the synthesis of biodiesel is carried out in a single transesterification step in a reactor that operates continuously, said process being ideal to be carried out at industrial scale since it can provide a continuous flow of biodiesel.

The invention relates to the use of microjet reactor for processing biomass. The cell lysis of flowable biomass is thereby carried out by means of multiple high-speed liquid jets which collide with one another, wherein the liquid jets contain the cells or consist wholly of the flowable cell mass, wherein intact or wholly or partially lysed biomass is added to at least one of the colliding high-speed liquid jets, and an extraction takes place simultaneously with the collision of the liquid jets or subsequently thereto. The lysis of the cells is initiated or facilitated by the forces that occur on acceleration, introduction of the acceleration, collision of the jets and mixing of the jet constituents.

A cell culture vessel comprising a housing in which a culture chamber is provided. In the housing, at least two holes that connect the outside of the housing and a culture chamber are provided.

Bioreactor and production process of adherent cell cultures that uses the bioreactor. The bioreactor includes a recipient with a cover, and further includes internally a bracket from which protrudes at least one tray that supports a three-dimensional polymer-based element mounted on it, such that when the bioreactor is filled with a culture medium the at least one tray and the three-dimensional polymer-based element are surrounded by the culture medium.

The process includes the following steps: the bracket, with the at least one tray of the bioreactor, with a three-dimensional polymer-based element is immersed in the culture medium, cells that are desired to be planted on the three-dimensional polymer-based element are inoculated from outside the bioreactor, and the culture is left in the bioreactor so that the cellular proliferation is produced.

There is provided a microfluidic system delivering external materials into a cell by cell mechanoporation using inertia, the microfluidic system including a fluidic channel structure through which a solution containing a cell and external materials flows continuously, in which the fluidic channel structure includes a junction between one or more channels, a localized vortex is generated near an interface of the junction, the cell is deformed by the vortex, and transient discontinuities are generated in a cell membrane by the vortex and the external materials are introduced into the cell by solution exchange between the cell and fluid around the cell.

The present disclosure provides a power device of a micro channel for external circulation of a bioreactor. The power device of the micro channel may be disposed outside the bioreactor and in fluid connection with the bioreactor, the power device of the micro channel may be of a shape of a box body. The power device of the micro channel may comprise: a stacked layer disposed in the box body, including a first shell plate, a second shell plate, and a sealing film sandwiched between the first shell plate and the second shell plate; and a liquid buffer device including a first liquid cavity and a second liquid cavity disposed in the box body, the first liquid cavity and the second liquid cavity may be fixed to an outer end surface of the stacked layer. The power device of the micro channel may be of a box shape, thereby reducing the volume and production cost thereof.

A microfluidic cell culture system is provided. The system includes a dual circulation arrangement for providing the cell culture with culture medium (and, optionally, selected compounds for study). The dual circulation arrangement permits culture conditions to be readily modified for different phases of cell culture. In particular, a first circulation route can be used to circulate a relatively high volume of medium, thereby allowing a low cell number to medium volume ratio, and a second circulation route can be used to circulate a relatively low volume of medium, thereby allowing a high cell number to medium volume ratio. The first circulation is optimised for a pre-culture period, before test compounds are added, and the second circulation is optimised for the test phase, providing a high cell number to medium ratio while preserving function during the test period.

The present invention relates to a microfluidic device for denudation of a cumulus oocyte complex. The device includes a substrate. A first channel having a width of about 200 μm to about 1 mm is located within the substrate. The first channel extends from a first end to a second end of the substrate. The first channel has a one or more ridge elements located along a surface thereof. A first port is located in the substrate and in fluid communication with the first end of the channel. A second port is located in the substrate and in fluid communication with the second end of the channel. Systems and methods of use of the microfluidic device for denudation of a cumulus oocyte complex are also disclosed.

A biomimetic system is provided for evaluating an effect of a test sample in vitro, and includes at least one organ chip, at least one medium container, a liquid pump, a nebulizer, a gas pump, and a chamber device. The liquid pump is provided to drive a liquid medium in the medium container to flow into a lower sub-channel of the organ chip and then to be discharged back into the medium container. The nebulizer is provided for atomizing a test solution including the test sample into an aerosol. The gas pump is provided to generate a pressurized gas which force the aerosol to flow out of a chamber of the chamber device and then to flow through an upper sub-channel of the organ chip.

The present invention concerns the field of high-efficiency, quality-controlled production of blue-green algae for direct human consumption, for extraction of proteins, vitamins, and amino acids, and for production of organic materials loaded with the special isotope 13C.

It is an object of the present invention to describe a high-efficiency photobioreactor.