Embodiments described herein generally provide for expanding cells in a cell expansion system. The cells may be grown in a bioreactor, and the cells may be activated by an activator (e.g., a soluble activator complex). Nutrient and gas exchange capabilities of a closed, automated cell expansion system may allow cells to be seeded at reduced cell seeding densities, for example. Parameters of the cell growth environment may be manipulated to load the cells into a particular position in the bioreactor for the efficient exchange of nutrients and gases. System parameters may be adjusted to shear any cell colonies that may form during the expansion phase. Metabolic concentrations may be controlled to improve cell growth and viability. Cell residence in the bioreactor may be controlled. In embodiments, the cells may include T cells. In further embodiments, the cells may include T cell subpopulations, including regulatory T cells (Tregs), for example.

Cell culture systems and methods provide improved immunotherapeutic product manufacturing with greater scalability, flexibility, and automation. Cell culture systems are configured with interchangeable cartridges, allowing versatility and scalability. Systems are configured to have multiple connected cell culture chambers, which allows parallel processing of different types of cells. Gas-impermeable cell culture chambers and methods for generating cells in closed systems prevent contamination and user error. Methods for recycling cell culture medium provide additional efficiencies.

Reactors, systems and processes for the production of biomass by culturing microorganisms in aqueous liquid culture medium circulating in a loop reactor which utilize substantially vertical flow zones are described. Recovery and processing of the culture microorganisms to obtain products, such as proteins or hydrocarbons is described.

A bioreactor including a bioreactor body, wherein the bioreactor body includes a first substrate and an opposing second substrate, a pathway extending through the bioreactor body and being formed by a first channel defined in the first substrate and an opposing second channel defined in the second substrate, a first inlet for introducing a first fluid flow to the first channel, a second inlet for introducing a second fluid flow to the second channel, a first outlet for permitting the first fluid flow to exit the first channel, a second outlet for permitting the second fluid flow to exit the second channel, a membrane disposed in the pathway between the first and second channels and having a plurality of pores sized to selectively capture, in the first channel, a biological source material and to permit biological products to be collected from the bioreactor.

Described herein are apparatuses and methods for culturing and monitoring cells in dynamic physiological conditions with combinatorial and biomechanical stimulation. In some embodiments, the apparatuses and methods may comprise one or more cell culture chambers, flexible membranes, pneumatic actuators, microfluidic layers, or hydrogels. In some embodiments, various mechanical stimuli including bending stress, shear stress, or a combination thereof may be applied to one or more cell types. Also described herein are high-throughput and dynamic cell culture array systems comprising the described apparatuses and methods.

Embodiments described herein generally provide for expanding cells in a cell expansion system. The cells may be grown in a bioreactor, and the cells may be activated by an activator (e.g., a soluble activator complex). Nutrient and gas exchange capabilities of a closed, automated cell expansion system may allow cells to be seeded at reduced cell seeding densities, for example. Parameters of the cell growth environment may be manipulated to load the cells into a particular position in the bioreactor for the efficient exchange of nutrients and gases. System parameters may be adjusted to shear any cell colonies that may form during the expansion phase. Metabolic concentrations may be controlled to improve cell growth and viability. Cell residence in the bioreactor may be controlled. In embodiments, the cells may include T cells. In further embodiments, the cells may include T cell subpopulations, including regulatory T cells (Tregs), helper, naïve, memory, or effector, for example.

Cell culture systems and methods provide improved immunotherapeutic product manufacturing with greater scalability, flexibility, and automation. Cell culture systems are configured with interchangeable cartridges, allowing versatility and scalability. Systems are configured to have multiple connected cell culture chambers, which allows parallel processing of different types of cells. Gas-impermeable cell culture chambers and methods for generating cells in closed systems prevent contamination and user error. Methods for recycling cell culture medium provide additional efficiencies.

The invention relates to a bioreactor (10, 10′, 100) adapted for rotation, the bioreactor comprising: a vessel (12) comprising: a first end (24) and a second end (26) which define a central axis (28) of the vessel (12) extending along a first direction, e.g. a length direction, of the vessel (12) from said first (24) to said second end (26), at least one wall (18, 18′, 20) running along the first direction of the vessel (12), at least one media conduit (22, 22′) defining a volume for receiving fresh or spent media; an inner chamber defined by at least a part of a space confined within said at least one wall (18, 18′, 20) and comprising a fresh media chamber (14) and a spent media chamber (16); a cell culture chamber (30) in fluid communication with said at least one media conduit (22, 22′) and said fresh (14) and/or spent media chamber (16); and a movable wall (38) configured, within said inner chamber, to separate said fresh media chamber (14) from said spent media chamber (16) within said inner chamber.

Cell culture systems and methods provide improved immunotherapeutic product manufacturing with greater scalability, flexibility, and automation. Cell culture systems are configured with interchangeable cartridges, allowing versatility and scalability. Systems are configured to have multiple connected cell culture chambers, which allows parallel processing of different types of cells. Gas-impermeable cell culture chambers and methods for generating cells in closed systems prevent contamination and user error. Methods for recycling cell culture medium provide additional efficiencies.

The present disclosure provides a power device of a micro channel for external circulation of a bioreactor. The power device of the micro channel may be disposed outside the bioreactor and in fluid connection with the bioreactor, the power device of the micro channel may be of a shape of a box body. The power device of the micro channel may comprise: a stacked layer disposed in the box body, including a first shell plate, a second shell plate, and a sealing film sandwiched between the first shell plate and the second shell plate; and a liquid buffer device including a first liquid cavity and a second liquid cavity disposed in the box body, the first liquid cavity and the second liquid cavity may be fixed to an outer end surface of the stacked layer. The power device of the micro channel may be of a box shape, thereby reducing the volume and production cost thereof.