The present disclosure describes a DNA fragment mixture for the prevention or for the treatment of at least one pathogenic, parasitic or infesting species of plants or of the environment, wherein the DNA mixture consists of random fragments of total DNA of at least one pathogenic, parasitic, or infesting species, and/or at least one phylogenetically similar species, against which the prevention and treatment are directed. Further, the disclosure describes a process and related system for improvement of the production/growth of microorganisms at high yield in bioreactors or photobioreactors, or of plants in different culture systems, where the nucleic acids of the organisms produced/grown by such a process are removed from the culture medium and the culture medium, deprived of these nucleic acid, is used again in the process.

A microfluidic system for cell retention for a perfusion bioreactor is provided. The system comprises at least one inlet configured to receive a bioreaction mixture to be processed. At least one curvilinear microchannel is in fluid flow connection with the at least one inlet, the at least one curvilinear microchannel being adapted to isolate cells in the bioreaction mixture, based on cell size, along at least one portion of a cross-section of the at least one curvilinear microchannel. At least two outlets are in fluid flow connection with the at least one curvilinear microchannel. At least one outlet of the at least two outlets is configured to flow the isolated cells to be recycled to the perfusion bioreactor.

Acoustic perfusion devices for separating biological cells from other material in a fluid medium are disclosed. The devices include an inlet port, an outlet port, and a collection port that are connected to an acoustic chamber. An ultrasonic transducer creates an acoustic standing wave in the acoustic chamber that permits a continuous flow of fluid to be recovered through the collection port while keeping the biological cells within the acoustic chamber to be returned to the bioreactor from which the fluid medium is being drawn.

Reactors, systems and processes for the production of biomass by culturing microorganisms in aqueous liquid culture medium circulating inner loop reactor which utilize nonvertical pressure reduction zones are described. Recovery and processing of the culture microorganisms to obtain products, such as proteins or hydrocarbons is described.

An exemplary embodiment of the present invention provides an apparatus for producing fermented soybean meal, which produces fermented soybean meal for monogastric animals and ruminant selectively or together. An apparatus for producing fermented soybean meal according to an exemplary embodiment of the present invention includes: a solid-liquid separating part, which mixes raw material soybean meal and an extraction solvent and extracts the soybean meal, and separately produces a remaining soybean meal and a soybean meal extract; a lactic acid bacteria culturing part, which produces the lactic acid bacteria by putting inoculum into the soybean meal extract, and supplies the lactic acid bacteria to the solid-liquid separating part; a solid substrate fermenting part, which is selectively supplied with and mixes at least two of the remaining soybean meal supplied from the solid-liquid separating part, the raw material soybean meal supplied through bypass, and lactic acid bacteria supplied from the lactic acid bacteria culturing part to produce a mixed material, and solid-substrate ferments the mixed material to produce primary solid substrate fermented soybean meal; and a drier, which dries the primary solid substrate fermented soybean meal supplied from the solid substrate fermenting part to produce secondary solid substrate fermented soybean meal.

Acoustic perfusion devices for separating biological cells from other material in a fluid mixture are disclosed. The devices include an inlet port, an outlet port, and a collection port that are connected to an acoustic chamber. An ultrasonic transducer creates an acoustic standing wave in the acoustic chamber that permits a continuous flow of fluid to be recovered through the collection port while keeping the biological cells within the acoustic chamber to be returned to the bioreactor from which the fluid mixture is being drawn.

We have modified a commercially-available adherent cell culture bioreactor, developing a new sampling manifold configuration and new way of taking samples to reduce contamination risk.

Acoustic perfusion devices for separating biological cells from other material in a fluid mixture are disclosed. The devices include an inlet port, an outlet port, and a collection port that are connected to an acoustic chamber. An ultrasonic transducer creates an acoustic standing wave in the acoustic chamber that permits a continuous flow of fluid to be recovered through the collection port while keeping the biological cells within the acoustic chamber to be returned to the bioreactor from which the fluid mixture is being drawn.

A method and system for high rate acidification and organic solids solubilization of feedstocks such as municipal source separated organics, municipal sewage sludge, and various industrial organic wastes are disclosed. The method and system feature a completely mixed bioreactor containing hydrogen-producing microorganisms, a crossflow membrane unit or membrane module located downstream of the bioreactor, a storage tank for receiving concentrated microorganisms from the membrane unit or module, and a connection that recirculates desired quantities of biomass from the storage tank to the bioreactor. This configuration decouples the solids residence time (SRT) from the hydraulic retention time (HRT) and results in a high solubilization rate.

The invention relates to a method and to a device for feeding gases or gas mixtures into a liquid, suspension, or emulsion in a reactor in a specific manner. According to the invention, gases or gas mixtures are fed into a liquid, suspension, or emulsion in a reactor in a specific manner, wherein the gas or gas mixture is fed in a specific amount and/or at defined points in time in one pulse as a gas bubble into a flowing liquid in a tilted reactor system, whereby a pulsation effect is obtained, wherein a driving force is produced by means of an adiabatic relaxation of the fed-in gas or gas mixture, by means of which driving force wall adhesions on the reactor are prevented.