A multi-stage anaerobic digester is designed to treat a high solids, stackable feedstock. The system may also receive a pumpable feedstock such as a slurry or sludge. In a first stage, the digestate circulates in one direction around a raceway such that the digestate may pass a feed inlet multiple times before leaving the first tank. An optional side stream loop withdraws fibrous material from near the top of the reaceway and return digestate with chopped fibers, preferably lower and further along the raceway. An outlet from the raceway located near, but upstream of the feed inlet discharges partially digested substrate to a second stage, which is operated as a stirred tank reactor. The two stages may be provided in a single tank with an internal wall separating a ring shaped outer portion from a cylindrical inner portion. The digester may be operated in a thermophilic temperature range.

Algae harvesting and cultivating systems and methods for producing high concentrations of algae product with minimal energy. In an embodiment, a dead-end filtration system and method includes at least one tank and a plurality hollow fiber membranes positioned in the at least one tank. An algae medium is pulled through the hollow fiber membranes such that a retentate and a permeate are produced.

A method may be provided for stimulating the growth of a biomass, which is mixed with a liquid inside a bioreactor, by means of ionising radiation. The following method steps are used: a) exposing a first partial volume of the liquid in the bioreactor to ionising radiation, the first partial volume comprising at most 10% of the liquid volume in the bioreactor; b) mixing the first partial liquid volume, which has been exposed to ionising radiation, with the second partial liquid volume, which has not been exposed to ionising radiation, in the bioreactor; c) repeating method steps a) and b) multiple times, each partial volume of the liquid in the bioreactor being exposed to a total radiation dose of at most 50 Gy on statistical average.

Systems and methods for containing and manipulating liquids, including vessels and unit operations or components of cell culture, cell containment, bioreactor, and/or pharmaceutical manufacturing systems, are provided. In certain embodiments, such vessels and unit operations are directed to continuous perfusion reactor or bioreactor systems and may include one or more disposable components. For instance, in one aspect, a system includes an apparatus in the form of a bioreactor for harvesting cells which produce one or more products. The apparatus may include a disposable, collapsible bag adapted for containing a liquid, the collapsible bag in fluid communication with a liquid-solids (e.g., cell) separation device. For example, an outlet of the collapsible bag may be connected to an inlet of the separation device, and an outlet of the separation device may be connected to an inlet of the collapsible bag for recycle. Accordingly, after separating the cells from the liquid in the separation device, the cells can be flowed back into the collapsible bag where they can be re-harvested. Meanwhile, product contained in the liquid can be collected in a separate container. The efficiency of product formation in such a system may be enhanced by using mixing systems described herein.

The subject invention provides methods and systems for producing microbe-based compositions that can be used in the oil and gas industry, environmental cleanup, agriculture, and many other applications. More specifically, the subject invention provides methods and systems for producing microorganisms and/or growth by-products thereof using a hybrid solid state-submerged fermentation method, wherein a matrix comprised of a solid material covered in an alginate coating is formed inside a liquid fermentation vessel. In some embodiments, the solid material is a plurality of natural loofa sponges.

The present invention is in the field of a method of producing lactic acid in high yield in a sequencing batch reactor. Therein glucose may be used as feedstock for bacteria, which ferment the glucose into lactic acid. The reactor is operated under at least partly defined non-axenic conditions and in a cyclic mode.

A biological component treatment system according to embodiments of the present disclosure includes an IC route through which the liquid is capable of flowing in inner cavities of hollow fibers, and an EC route through which the liquid is capable of flowing in a main space on an outer side of the hollow fibers. The biological component processing system is further equipped with a control unit that controls a flowing state of the liquid through the IC route and the EC route. The control unit, at a time of priming, while the liquid flows through both the IC route and the EC route, causes a differential pressure to be generated between the liquid flowing through the inner cavities and the liquid flowing through the main space, and causes the gas to flow out from the hollow fibers.

A bioreactor with a closed vessel, a reaction chamber, and at least one barrier, wherein the bioreactor is configured to support a biologically active environment. The barrier for the reaction chamber of the bioreactor includes pores and a media flow path connected to the pores. The media flow path is configured to have a diameter that is smaller than the average diameter of the pores. The barrier of the bioreactor includes a surface feature, internal structural feature, or combinations thereof, which are configured to improve or enhance the growth of cells or microorganisms.

Disclosed is a mass-cultivation system for microalgae, including a reactor that contains a cultivation liquid in the interior thereof, wherein the liquid includes functional particles. According to the mass-cultivation system for microalgae according to the present invention, because various functions that are necessary for cultivation of microalgae may be uniformly distributed in a cultivation liquid by allowing functional particles having various functions to flow in the cultivation liquid, a suitable environment may be created based on the cultivation of a large amount of microalgae and the growth of microalgae so that a high efficiency cultivation system may be realized while the problems of mass-cultivation of an existing cultivation system may be solved.

The present disclosure provides a power device of a micro channel for external circulation of a bioreactor. The power device of the micro channel may be disposed outside the bioreactor and in fluid connection with the bioreactor, the power device of the micro channel may be of a shape of a box body. The power device of the micro channel may comprise: a stacked layer disposed in the box body, including a first shell plate, a second shell plate, and a sealing film sandwiched between the first shell plate and the second shell plate; and a liquid buffer device including a first liquid cavity and a second liquid cavity disposed in the box body, the first liquid cavity and the second liquid cavity may be fixed to an outer end surface of the stacked layer. The power device of the micro channel may be of a box shape, thereby reducing the volume and production cost thereof.