Embodiments of the present disclosure relate to systems and methods for ethanol production, use, and waste recovery using aquatic plants. In certain embodiments, systems and methods are disclosed for reuse and further processing of waste alcohols, sugars, organic acids and/or byproducts produced by conversion of corn, other grains, or other biomass materials of use in biofuel production methods.

Reactors, systems and processes for the production of biomass by culturing microorganisms in aqueous liquid culture medium circulating in a loop reactor which utilize substantially vertical flow zones are described. Recovery and processing of the culture microorganisms to obtain products, such as proteins or hydrocarbons is described.

A method for providing a large amount of cell culture medium (10), in particular for cultivating animal cells, for a large reactor system (12) by using single-use components, comprises the following sequence of steps: a) preparing a concentrated media stock solution (32), b) filling the concentrated media stock solution (32) into single-use containers (40), c) transporting the single-use containers (40) filled with media stock solution (32) to the large reactor system (12), d) aseptically connecting the single-use containers (40) to the large reactor system (12) by means of a single-use hose system (26), e) processing the media stock solution (32) by supplying ultrapure water (24) and/or additives (44), and f) supplying the processed media stock solution (32) into the large reactor system (12).

Control of humidity in chemical reactors, and associated systems and methods, are generally described. In certain embodiments, the humidity within gas transport conduits and chambers can be controlled to inhibit unwanted condensation within gas transport pathways. By inhibiting condensation within gas transport pathways, clogging of such pathways can be limited (or eliminated) such that transport of gas can be more easily and controllably achieved. In addition, strategies for purging condensed liquid from chemical reactor systems are also described.

The disclosure relates to bioreactors, for example for biological treatment and, more specifically to bioreactor insert apparatus including biofilms and related methods. The bioreactor insert apparatus provides a means for circulation of reaction medium within the bioreactor, a biofilm support, and biological treatment of an inlet feed to die reactor/insert apparatus. The bioreactor insert apparatus has a high relative surface area for biofilm attachment and is capable of generating complex flow patterns and increasing treatment efficiency/biological conversion activity in a biologically-active reactor. The high surface area structure incorporates multiple biofilm support structures such as meshes at inlet and outlet portions of the structure. The biofilm support structures and biofilms thereon can increase overall reaction rate of the bioreactor and/or perform some solid/liquid separation in the treatment of the wastewater or other influent.

Some fluid coupling devices described herein are configured for use in fluid systems. For example, some embodiments described in this document are single-use, aseptic fluid coupling devices that can be coupled to create a sterile flow path therethrough. Some such aseptic couplings are genderless couplings such that two identical aseptic couplings can be coupled together and then latched to form a robust connection between the aseptic couplings.

The monitoring and control of bioprocesses is provided. More particularly, the present disclosure is directed to formulating buffer products from multiple buffer solutions for feeding the different operations occurring within a bioprocess line. As the buffer product is being formulated, the buffer product is tested for conductivity, refractive index, and optionally pH. The conductivity measurements are used in conjunction with refractive index measurements to ensure that the buffer product not only has the correct concentration of ions but also has the correct concentration of components. A controller can be used to make automatic adjustments to the buffer product should any of the measured parameters fall outside a preset range.

Embodiments of the present disclosure are directed to systems, apparatuses, devices and methods for tissue cultivating. Such systems may comprise a plurality of bioreactors, each bioreactor comprising a bag having a mandrel tube arranged therein, and a fluid management system for managing fluid flow among the plurality of bioreactors. The fluid management system can include at least one intra-luminal pump, configured to flow intra-luminal fluid in a first direction in the bioreactors, at least one media pump, configured to flow biomedia fluid in the bioreactors in a second direction opposite to the first direction, and a plurality of valves and/or clamps to effect at least one of filling, flowing, cessation of flow, and draining of at least one of the intra-luminal fluid and biomedia fluid tubes among the bioreactors.

Provided is a cell culture apparatus including a cell supply unit that supplies cells; a culture medium supply unit that supplies a culture medium; an additive supply unit that supplies an additive for inducing the differentiation of undifferentiated cells; a stirring unit that stirs a processing target; a separation unit that separates a component contained in the processing target; a culture vessel that cultures the cells; a first flow channel that forms a circulation route passing through the cell supply unit, the stirring unit, the separation unit, and the culture vessel; a second flow channel that connects the culture medium supply unit and the first flow channel; a third flow channel that connects the additive supply unit and the first flow channel; and a control unit that controls the feeding of liquid through the first flow channel, the second flow channel, and the third flow channel.

Provided is a cell culture apparatus including a culture vessel that stores a cell suspension containing cells; a first filter part that has a first filter membrane that performs membrane separation treatment on the cell suspension extracted from the culture vessel; a first circulation flow path that allows components blocked by the first filter membrane to return to the culture vessel; a second filter part that has a second filter membrane that performs membrane separation treatment on components of the cell suspension permeated through the first filter membrane; a second circulation flow path that allows components permeated through the second filter membrane to return to the culture vessel; and a recovery flow path that recovers components blocked by the second filter membrane. In the cell culture apparatus, an average hole diameter of the first filter membrane is 20 m or smaller, and 0<B/A0.5 is satisfied in a case where an average hole diameter of the first filter membrane is A and an average hole diameter of the second filter membrane is B; or an average hole diameter of the first filter membrane is 20 m or smaller, and the second filter membrane is an ultrafiltration membrane.