The present disclosure relates to a reactor that includes a cylinder integrally connected to a top portion and a bottom portion, where the bottom portion is rounded and configured to contain a stir bar, the top portion includes a neck configured to receive a cap, the cylinder has a height (H) and a width (W) such that the ratio of H to W is between about 1:1 and about 10:1, and the cylinder is transparent to light having a wavelength between about 400 nm and about 700 nm.

An apparatus for enhancing bacterial sporulation comprising a laminar flow chamber including a plurality of laminar flow tubes that smooth flow of coolant, a coolant input port that is coupled to the laminar flow chamber that supplies the coolant to the laminar flow chamber from a coolant source, a transparent tube section that is coupled to the laminar flow chamber and includes light-emitting diode (LED) light modules, the transparent tube section receives the smoothed flow of coolant from the laminar flow chamber, a culture solution tube that traverses through the laminar flow chamber and the transparent tube section, wherein a portion of the culture solution tube that is within the transparent tube section is surrounded by the coolant and is exposed to light from the LED light modules, and a culture solution input port that is coupled to the culture solution tube and supplies a culture solution to the culture solution tube.

A multi-layer culture vessel observation system, a cart device, and a multi-layer culture vessel observation device that enable an operator to easily observe objects to be observed in a multi-layer culture vessel are provided. A multi-layer culture vessel observation system includes: a cart device 20 capable of carrying a multi-layer culture vessel 30 that includes a plurality of trays and moving; and an observation device 10 capable of observing objects to be observed in each tray in the multi-layer culture vessel 30, wherein the cart device 20 includes a frame body including a sideways exposure part that exposes two side faces of the multi-layer culture vessel 30 from an upper end to a lower end, wherein the observation device 10 includes: a storage 14 that stores the cart device 20 in a state of carrying the multi-layer culture vessel 30; and an imaging device 11 that includes an optical system and outputs an image formed by the optical system, and wherein, when the cart device 20 carrying the multi-layer culture vessel 30 is stored in the storage 14, the sideways exposure part is located on an optical axis of the imaging device 11.

An observation device includes: an illumination unit that generates illumination light to be radiated onto cells floating in a culture fluid inside a culture vessel; a light receiving unit that receives observation light coming from the cells that have been irradiated with the illumination light, the observation light being imaged by an image-forming optical system; and a casing that has a transmissive section so as to transmit the illumination light and the observation light and that accommodates the illumination unit and the light receiving unit, wherein the casing has an elongated cylindrical form that is configured to be inserted into the culture fluid via a port used to insert a tube into the culture vessel.

Embodiments of a modular tubular bioreactor system for culturing an aqueous culture of microorganisms are described herein. The tubular bioreactor may comprise culture tubes configured in a vertically spaced and horizontally staggered arrangement to optimize the application of light to the culture in phototrophic and mixotrophic cultivation.

A method of killing specific cells from among a group of cells cultured in a culture vessel by quick and brief laser treatment, the cell culture vessel comprising a main body and a to-be-irradiated layer attached to the main body, the to-be-irradiated layer containing an ingredient capable of absorbing laser light upon laser irradiation, the group of cells being cultured on the surface of the to-be-irradiated layer, the method comprising: applying laser light to a partial area of the to-be-irradiated layer directly below the specific cells.

A biocompatible composite element for a bioreactor is provided that includes an outer frame and an inner component. The outer frame is a polymeric material. The outer frame can be inseparably attached to a wall of the bioreactor. The inner component is a transparent material selected from a group consisting of glass, sapphire, and glass ceramic. The inner component is secured in the outer component in an inseparable hermetically tight manner. The inner component is configured for a spectral process control through the transparent material of the inner component.

A cell culture carrier includes a stimulus-responsive substrate for cell culture comprising a stimulus-responsive polymer and an external signal receiving material, an active substance is supported on the stimulus-responsive substrate for cell culture.

Embodiments described herein provide systems for optimizing organism growth, destruction or repair, by controlling the duty cycle, wavelength band and frequency of photon bursts to an organism, through the high frequency modulation of photons in an individual color spectrum to the organism, where the photon modulation is based upon the specific needs of the organism. Devices for the optimization of organism growth, destruction or repair through the high frequency modulation of photons of individual color spectrum to the organism are also provided. Further provided are methods for the optimization of organism growth, destruction or repair through the use of high frequency modulation of photons of individual color spectrums.

Disclosed herein are bioreactors that include a container for holding fluid aligned on a vertical axis, wherein at least a portion of the container is oriented at an angle relative to the vertical axis, wherein the angle is from about 0 to 90. In some embodiments, the container is placed on a support structure with portions of the container oriented at alternating angles. The system is essentially closed except for at least one opening (for example an opening at the bottom edge) that allows for the introduction of gases and/or nutrients. The gas and/or nutrients are introduced in such a way as to provide mixing and aeration of a cell culture in the bioreactor. Also disclosed are methods of culturing cells (such as microalgae, macroalgae, bacteria, fungi, insect cells, plant cells, animal cells, or plant or animal tissue or organs) using a bioreactor described herein.