Atmospheric carbon has risen above 400 parts per million (ppm) crossing a threshold that endangers climate stability. Greenstone Device biotechnology enables consumers, businesses, municipalities and nations to mitigate their carbon output by providing a platform for capturing emitted carbon directly from the atmosphere, and converting that carbon into organic biomass fertilizer.

A photobioreactor device for growing photosynthetic organisms, organisms that feed on photosynthetic organisms and hydroponics is disclosed. The device may include a lighting system integrated around the circumference of the device. In embodiments, a cap configured to seal the container has ports configured to permit passage of fluid out of or into a container without removing the cap. In some embodiments, organisms are grown in a disposable bag secured between the cap and the container.

Bioreactors and methods for cultivating microalgae are provided herein. The bioreactor and methods include features and modifications to improve heterotrophic growth efficiency by providing a light signal.

Disclosed is a device for exposing an algal solution to light, for a photobioreactor, this exposure device including a flat support including at least one opening capable of holding at least one shaft, the shaft being made of a liquid-tight, translucent or even transparent flexible material, and including an open end capable of holding a sleeve, the sleeve being translucent, or even transparent, and arranged to seal and attach the shaft to the support. The exposure device has positive floatability in water. Also disclosed is a photobioreactor equipped with such a device and a start-up method for such a photobioreactor.

Provided are systems and methods for spatially and temporally controlling light with an illumination device comprising a light source operably connected to a circuit board, one or more light guide plates, one or more optical masks, a controller, and a computer readable medium, comprising instructions that, when executed by the controller, cause the controller to: illuminate a cell or a substrate with light from the light source, and spatially and temporally control illumination of light to the cell or the substrate with one or more illumination parameters, wherein the one or more light guide plates provides uniform illumination of the light. Also provided herein are methods of screening using the system and/or device of the present disclosure.

Provided is a cell-culturing apparatus including: an incubator that can maintain an interior thereof in an environment that is appropriate for growth of cells; a cell-culturing vessel that is accommodated inside the incubator; an optical-data acquisition unit that acquires optical data of a medium in the cell-culturing vessel; a medium changing unit that changes the medium in the cell-culturing vessel; and a controller, wherein a timing at which the medium is changed is determined on the basis of a change over time in the optical data acquired by the optical-data acquisition unit.

A photobioreactor system includes a frame, a reflective wall coupled to the frame such that the reflective wall defines and circumferentially surrounds an interior chamber, and multiple light sources coupled to the reflective wall such that the multiple light sources circumferentially surround the interior chamber. The interior chamber is configured to receive a container that holds and supports growth of a desired organism.

A system for growing and reproducing microorganisms that includes a basin system that includes a number of basins, where each basin has a vertical meandering system which is formed by partitions and which can be illuminated, each basin is filled with a nutrient suspension, at least one outer wall of each basin is double-walled such that a cavity is formed, a temperature control medium for controlling the temperature of the nutrient suspension can flow through the cavity.

A container (1, 1b) of a bioreactor (1a, 1b) has a container interior (22) designed for being filled with a medium (8) including a photoreactive material, and designed for at least partially triggering a photochemical reaction of the photoreactive material. The container (1, 1b) has at least one illuminant receiving pouch (11) that is at least partially transparent for an electromagnetic radiation (14), and that is arranged on a illuminant opening (12) within the container interior (22) and is designed to receive at least one illuminant (15, 15a, 15b, 15c, 15d, 15e) from an outer side (A) through the illuminant opening (12, 12a, 12b). The illuminant at least partially irradiates the medium (8) such that the photochemical reaction of the photoreactive material can be triggered by electromagnetic radiation (14) emitted by the illuminant and the illuminant is isolated in relation to the medium (8) by the illuminant receiving pouch (11).

Bioreactors and methods for cultivating microalgae are provided herein. The bioreactor and methods include features and modifications to improve heterotrophic growth efficiency by providing a light signal.