C12M33/04

Axenic inoculation system for microalgae using TR tube of triiodide resin and method for culturing axenic culture

A triiodide resin (TR) tube of the present invention has a cylindrical shape. A resin exhaust preventing part is provided at a lower portion of the TR tube, and a triiodide resin is received inside the TR tube. A contaminated culture passes through the TR tube to allow an axenic culture to flow out. When a microalgae culture contaminated with bacteria passes through the TR tube filled with the triiodide resin, a pure culture of microalgae can be prepared by using the sterilizing rate of bacteria populations, which is relatively higher than that of microalgae, due to characteristics of iodine exhibiting negative polarity. An axenic inoculation system for microalgae using the TR tube of the present invention comprises the steps of: sterilizing a photobioreactor (PBR) with peracetic acid, washing the PBR with purified water, and filling the PBR with a sterilized culture; allowing a microalgae culture contaminated with bacteria to pass through a TR tube attached on an upper portion of the PBR, thereby obtaining an axenic culture; and inoculating the axenic culture into a culture liquid inside the PBR to culture microalgae. According to the present invention, the microalgae contaminated with bacteria are sterilized together with bacteria in external air flowing in the TR tube while passing through the TR tube, thereby causing no secondary contamination at the time of inoculation or culture.

DRIVE MECHANISM CAPABLE OF DEALING WITH GAS STERILIZATION
20220033201 · 2022-02-03 ·

Provided is a drive mechanism capable of withstanding sterilization treatment using a sterilization gas such as hydrogen peroxide and capable of performing positioning operation with heightened accuracy. In a drive mechanism according to the invention, a movable block disposed in the internal space of a partition wall for blocking a sterilization gas is coupled to a movable platform disposed in the external space of the partition wall by means of a magnet coupling mechanism. Further, biasing magnet units are disposed on surfaces, of the movable block and the movable platform, on which no magnet coupling mechanism is disposed, such that the biasing magnet units serve as magnetic poles repelling each other. Repelling force generated by the biasing magnet units serves as biasing force that biases the movable platform toward the partition wall.

Apparatus for culturing cells

An apparatus for culturing cells of the present disclosure includes: a cabinet that has a main surface and a side surface, the main surface having a window; a main surface aspiration port disposed at the main surface of the cabinet; and a side surface aspiration port disposed at the side surface of the cabinet. In the cross section of the cabinet taken along the horizontal direction, a first vessel supplying part for supplying a first vessel is disposed at a midportion of the cabinet, and a second vessel supplying part for supplying a second vessel is disposed at an inner peripheral portion of the cabinet. The first vessel supplying part has a first lid capable of entirely covering the first vessel supplying part. The first vessel has no lid. The second vessel supplying part has no lid. The second vessel has a second lid.

MICROORGANISM INOCULATION DEVICE
20220033754 · 2022-02-03 ·

Disclosed is a microorganism inoculation device, which comprises a workbench, wherein support columns are provided on the upper surface of the workbench, a limiting plate is provided in the middle part of the supporting column, an injection head is provided in the middle part of the limiting plate, an air cylinder is provided in the middle part of the lower surface of the workbench, a piston rod of the air cylinder is fixedly connected with the middle part of the upper surface of a lifting plate, guide rods are provided on the upper surface of the lifting plate, the upper end of the guide rod is fixedly connected with a tray, the left and right sides of the upper surface of the tray are fixedly connected with the lower part of a fixed bracket, the middle part of the injection head is slidably connected with the limiting plate.

Apparatus for the transfer of bio-ink

An apparatus for transferring bio-ink onto a target having a slide defining a receiving area of a film of fluid containing inhomogeneities, a laser source associated with controlled diversion means and an optical block for focusing in a plane of the fluid film in order to apply a local pulse, wherein the apparatus also comprises imaging means and means for analyzing images in order to recognize the geometric positions of the inhomogeneities in the film, and an observable feature of each of the inhomogeneities (size, shape factor, type of particles, age of the particle, density, type of biomaterial, molecule, etc.) recognized by the appropriate analysis means. The apparatus further comprises selection means for selecting at least one of the inhomogeneous areas, and means for controlling the diversion in order to direct the laser beam toward the position of the inhomogeneous area and trigger the firing of the laser.

CELL PICKING DEVICE
20220268794 · 2022-08-25 ·

A cell picking device includes a supporter that supports a rack having a plurality of holes for holding a plurality of pipette tips, a sucker used to suck a sample, a driver that moves the sucker in an up-and-down direction and moves the sucker and the supporter relative to each other in a horizontal direction, and a controller that controls the driver such that, any pipette tip out of the plurality of pipette tips held by the rack is attached to the sucker by movement of the sucker in the up-and-down direction and the sucker is moved to a position outwardly of the rack by movement of the sucker and the supporter relative to each other.

DEVICE, SYSTEM, AND METHOD FOR SELECTING A TARGET ANALYTE
20170276575 · 2017-09-28 · ·

This disclosure is directed to a device and a system for picking a target analyte of a suspension. A picker introduces at least one force, such as by a magnetic gradient and/or by a pressure gradient, to extract the target analyte from a sample.

CELL TRANSPLANTATION DEVICE AND CELL TRANSPLANTATION SYSTEM

A cell transplantation device is used to place a graft containing cells into a target region in a living body. The cell transplantation device includes a needle extending in one direction, and a filter unit made of a fibrous member. The needle has a flow channel defined therein and an opening at the tip of the needle. The filter unit is disposed at an intermediate position in the flow channel and configured to retain a graft between the filter unit and the opening when a liquid material containing the graft enters the flow channel through the opening.

CONNECTOR

There is provided a connector, for introducing or extracting a material to or from at least one receptacle, comprising a housing extending between a distal end and a proximal end, the housing comprising, at least at one end, a pierceable seal; a hollow needle mounted, at least partially, within the housing between the distal end and the proximal end of the housing, a first end of the hollow needle being connected or connectable to a first corresponding receptacle, and a second end of the hollow needle facing the pierceable seal at an end of the housing; and an actuating mechanism acting on the housing or the hollow needle to enable the hollow needle to pierce the pierceable seal thereby forming a communication through the pierceable seal, such that material is able to transfer through the connector.

SAMPLING SYSTEM AND METHOD OF USING THE SAME
20210388304 · 2021-12-16 ·

The present application is directed to a sampling system for sampling a fluid from a vessel, where the sampling system includes a sterile dispenser assembly operatively connected to the vessel, the sterile dispenser assembly including a valve operatively connected to the vessel, a membrane, and a needle, and a detachable sterile sampling container assembly operatively connected to the sterile dispenser assembly, the detachable sterile sampling container assembly including a sampling container, a membrane attached to the sampling container, and a sampling container housing enclosing the sampling container, where the sampling container housing includes a compressible portion having a deflated configuration and an expanded configuration.