A bone fragment and osteomedullary tissue harvesting system that includes a harvesting device, a collection vessel and tubing. The harvesting device includes a needle portion and a handle portion. The needle portion that has a needle bore that extends through at least part of the needle portion. The handle portion is operably attached to the needle portion. The handle portion includes a connection port and a vacuum control mechanism that are in communication with a handle bore that extends through the handle portion. The needle bore is in communication with the handle bore. The vacuum control mechanism includes a vacuum aperture that extends through a surface of the handle portion and is in communication with the handle bore. The collection vessel is capable of receiving aspirated bone fragments and tissue. The tubing operably connects the connection port and the collection vessel.

An adapter assembly for receiving a capillary includes a passage, into which the capillary is or can be received. The adapter assembly has an adapter cone which is configured for being received in or on a correspondingly configured counter cone. The adapter cone, at least over a section for bringing the adapter cone into contact with the counter cone, includes an elastic material that is more elastic than the material of the counter cone. The invention further relates to a counterpart for connecting to the adapter, to an apparatus and to a method for automatically exchanging capillaries.

A cell detection device that detects a cell in a specimen with high sensitivity and includes a sealed container having a sealable specimen introduction portion, a culture portion that holds a culture solution, an extraction reagent portion that holds an extraction reagent, and a luminescent reagent portion that holds a luminescent reagent, a contact mechanism that controls contact between the culture solution, the extraction reagent, and the luminescent reagent, a photodetector that detects luminescence from the luminescent reagent portion, and a calculator that determines growth of a cell based on a detection signal of the photodetector. The culture solution, the extraction reagent, and the luminescent reagent are separately disposed in the sealed container, and the contact mechanism brings the culture solution to which a specimen is added and the extraction reagent into contact to obtain an extraction solution, and intermittently brings the extraction solution and the luminescent reagent into contact.

A jig member for use in the manufacture of a luminal cell structure, in which needle-shaped bodies are arranged in a comb-like form on a thickness-direction surface of a plate-like support having a length direction, wherein a surface which is adjacent to the thickness-direction surface and is along the length direction is formed in a tapered shape toward tips of the needle-shaped bodies; and a jig for use in the manufacture of a luminal cell structure, in which the member is arranged on a circumference of a circle with the tips of the needle-shaped bodies facing in the direction toward the center of the circle.

A sampling system includes a graduated sampling chamber configured for fluid connection to a sample source, a pump device configured for fluid connection with the sampling chamber, and a sterile air filter intermediate the pump device and the sampling chamber, wherein the pump device is selectively actuatable to draw a volume of fluid from the sample source into the sampling chamber.

Delivering a payload across a plasma membrane of a cell includes providing a population of cells and contacting the population of cells with a volume of an aqueous solution. The aqueous solution includes the payload and alcohol content greater than 5 percent concentration. The volume of the aqueous solution may be a function of exposed surface area of the population of cells, or may be a function of a number of cells in the population of cells. Related compositions, apparatus, systems, techniques, and articles are also described.

An object of the present invention is to purify and concentrate differentiating cells derived from ES cells, iPS cells, or the like without damaging them. The above problem can be solved by an apparatus for analyzing and separating particles comprising: a flow path cartridge, an illumination unit, a detection unit for detecting particles of interest, a force generating unit, wherein a sample liquid reservoir (sample reservoir) connected to a first flow path; a fourth branched flow path and a fifth branched flow path which are connected to the first flow path; a third-A reservoir connected to the fourth branched flow path; a third-B reservoir connected to the fifth branched flow path; and a fourth reservoir for reserving particles which are not sorting; are formed on the cartridge, and each reservoir comprise a means which equalizes an air pressure in the each reservoir with an air pressure of an in-device air pressure control system, and a stream of the flow path in the cartridge is controlled by controlling the air pressure in the each reservoir through the each in-device air pressure control system.

Aspects of the invention relate to liquid transfer systems including a pipette tip having a body defining an inner volume and having first and second ends, the first end arranged to transfer a volume of liquid into and out of the pipette tip and the second end arranged for attachment to a pipette, and a bladder sealingly attached to the body. In some embodiments, the bladder is disposable in the inner volume. The bladder may be defined by a membranous sac and/or a planar membrane. The bladder may be moveable in response to an applied pressure to transfer liquid. The bladder also may be stretchable to accommodate a volume of fluid. In some embodiments, the pipette tip is used in a cell culture incubator, with a manipulator including a pipette that is in fluid communication with the pipette tip.

An electroporation system may include a well plate, a dispenser and a dispenser-well positioning system. The well plate may include wells, each of the wells including an interior, a first electrode adjacent the interior and a second electrode adjacent the interior and spaced from the first electrode. The first electrode and the second electrode are to apply an electrostatic field across the well. The dispenser is to dispense a cell having a diameter into each of the wells. The dispenser-well positioning system is to align each well and the dispenser such that the dispenser dispenses the cell into each well at a location spaced from the first electrode and the second electrode by a distance of at least 5 times the diameter of the cell.

A continuous automated perfusion culture analysis system (CAPCAS) comprises one or more fluidic systems configured to operate large numbers of biodevices in parallel. Each fluidic system comprises an input reservoir plate for receiving media; a biodevice plate comprising an array of biodevices fluidically coupled to the input reservoir plate, configured such that each biodevice has independent media delivery, fluid removal, stirring, and gas control, and each biodevice is capable of continuously receiving the media from the input reservoir plate; and an output plate fluidically coupled to the biodevice plate for real-time analysis and sampling. The operations of the CAPCAS are automated and computer-controlled wirelessly. The CAPCAS can also be used for abiotic and biotic chemical synthesis processes.