Acoustophoretic devices and methods for separating biological cells (particularly T-cells) from other fluids/materials using multi-dimensional acoustic standing waves are disclosed. The devices include an inlet, at least two outlets, and a flow chamber having an ultrasonic transducer-reflector pair. Specifically, T cells, B cells, or NK cells can be separated from other blood components. A dual-pass acoustophoretic system including two acoustophoretic devices arranged in series and fluidly connected to one another is also illustrated. Means for pre-chilling the mixture prior to separation in the devices or system can be used to improve retention, concentration, and clarification and to prevent outgassing.

Provided is a cell detachment device causing to know whether the vibration applied to a culture vessel and the cells under the desired condition is applied. The device generating a first vibration and detaching a culture cell from a culture vessel using the first vibration, comprises: a piezoelectric element in which a second vibration is excited by applying a first alternating current using a first and second electrodes; a vibrated system in which the second vibration propagates, including a vibration member to which the piezoelectric element is fixed and on which the culture vessel mounted; a third electrode used outputting a second alternating current excited on the piezoelectric element by the first vibration generated in the vibrated system caused by the second vibration; and a detection unit detecting a state of the first vibration generated in the vibrated system by using the second alternating current output from the third electrode.

The current invention relates to a method for obtaining one or more target biomolecules from cells cultured in a fixed bed bioreactor, said method comprises: the addition of a lysis solution to the bioreactor, thereby lysing the cells in the bioreactor, and prior to, simultaneous with, and/or after lysing said cells, applying one or more mechanical actions to said bioreactor, after which the one or more target biomolecules are recovered from said bioreactor. The invention further relates to a system for obtaining one or more target biomolecules from cells cultured in a fixed bed bioreactor.

Microparticles and nanoparticles made of various materials that are used in various configurations are disclosed. Such particles can also contain various types of materials as payloads to be used in the separation, segregation, differentiation, modification or filtration of a system or a host anatomy. The microparticles and nanoparticles are utilized in conjunction with an acoustic standing wave or an acoustic traveling wave in various processes.

A recipient for cell cultivation having an inner compartment adapted for cell growth, in one embodiment, an outer tubular wall extends in a longitudinal direction and delimits an outer boundary of the inner compartment in a radial direction. First and second ends delimit the inner compartment at the first respectively the second outer end of the outer tubular wall. A fixed packing in the inner compartment comprises a packing, such as a fiber matrix. Additional embodiments and related methods are also disclosed.

An apparatus for growing biological material, comprising a bioreactor chamber comprising at least one substrate positioned within an interior of the bioreactor chamber to support the growth of the biological material; at least one inlet to supply nutrient; and at least one outlet configured to enable biological material to be retrieved from the bioreactor chamber; a liquid nutrient container comprising at least one liquid nutrient outlet fluidly connected to the bioreactor chamber to supply a liquid nutrient; and an atomizer fluidly connected between the bioreactor chamber and the liquid nutrient container and comprising a carbon dioxide inlet fluidly connected to receive a source of carbon dioxide wherein the atomizer is configured to receive liquid nutrient from the liquid nutrient container and produce a liquid nutrient mist and provide a mixture of the liquid nutrient and the carbon dioxide from the carbon dioxide inlet to the bioreactor chamber.

Methods and devices for manipulating one or more particles (e.g., cells) in three dimensions using surface acoustic waves is described. Methods and devices for printing or more biological cells onto a substrate using surface acoustic waves are also provided.

In an embodiment of the invention, there may be provided a bioreactor having tissue scaffolds and having culture medium perfused therethrough. There may be multiple independent culture chambers and reservoirs or sub-reservoirs. Sensors can provide for individually controlling conditions in various culture chambers, and various culture chambers can be operated differently or for different durations. It is possible to infer the number of cells or the progress toward confluence from the fluid resistance of the scaffold, based on flowrate and pressure drop. Harvesting may include any combination or sequence of; exposure to harvesting reagent; vibration; liquid flow that is steady, pulsatile or oscillating; passage of gas-liquid interface through the scaffold. Vibration and flow can be applied so as to reinforce each other.

Systems and methods are disclosed herein for use in transducing, activating, and otherwise treating cells. Cells are introduced into an inner layer of a multi-layered stack that defines at least one flow chamber and a plurality of cell entrainment regions. Vertical flow through the stack entrains the cells in the cell entrainment regions along with genetic information introduction agents or other additives, before the cells are washed using a reverse vertical flow and are collected from the device.

Systems and methods for harvesting cells include a bioreactor with a structure for cell entrapment/adherence and growth, such as a fixed bed structure. A cell harvest mechanism is adapted to agitate the bioreactor, such as by vibrating or shaking the fixed bed, and to move a liquid level relative to the structure, such as by repeatedly flushing and filling the bioreactor. A cell detaching solution is provided to the bioreactor, such as an enzymatic cocktail for the detachment of cells without producing clumps or aggregates. The bioreactor may comprise a preculture vessel upstream of another bioreactor.