A centrifuge rotor having a curved shape is offset on a spinning rotor base and creates contiguous areas of low to high centrifugal force depending on the distances from the axis of the rotor base and a method of separating components in a fluid based upon a difference in density of the components, the method comprising the steps of providing to a rotor as described herein the fluid containing the mixed together components to be separated based upon the difference in density of the mixed together components; continuously flowing the components in the fluid to the rotor through an input tube connected to the input port while the rotor is spinning about a centrifugal axis of rotation; separating the components in the fluid into fractions based upon the difference in density of the mixed together components with the use of centrifugal force when the rotor is spinning; collecting components having i) a first density via a first tube connected to the output port at the first end on the rotor, ii) a second density via a second tube connected to the output port at the second end on the rotor, iii) a third density via a third tube connected to the output port at the junction on the rotor and iv) a fourth density via a fourth tube connected to the output port between the input port and the output port at the first end.

A cover lid collector (10) for a sample carrier (40) for cell cultivation, wherein the cover lid collector (10) comprises a connecting portion (12) for engaging with the sample carrier (40) for closing the same, wherein the connecting portion (12) defines a first plane (11). The cover lid collector (10) further comprises a cavity (14), which when the cover lid collector is engaged with the sample carrier (40), has a cross-section that decreases in a direction perpendicular to the first plane (11) and pointing away from said sample carrier (40) between a near end (16) of the cavity (14) and a far end (18) of the cavity (14), wherein the near end (16) is closer to the sample carrier (40) than the far end (18).

The present disclosure relates to a method for attaching, detecting and retrieving a single cell on a manipulation medium comprising the steps of: attaching the single cell onto the manipulation medium by applying a carrier liquid; partially drying out the carrier liquid; detecting a property of the single cell by rotating the manipulation medium and scanning a light beam over the manipulation medium; loosening the single cell from the manipulation medium without damaging the single cell by manipulating the single cell with an instrument; and retrieving the single cell from the manipulation medium by aspirating the single cell into a tube, wherein the carrier liquid is dried out to a degree corresponding to that the single cell does not move during the rotation of the manipulation medium and that the single cell can be loosened by the instrument without damaging the single cell. The disclosure further relates to an apparatus for detection and manipulation of one or more object(s).

The invention relates to a system, comprising: a) a sample processing unit, comprising an input port and an output port coupled to a rotating container having at least one sample chamber, the sample processing unit configured provide a first processing step to a sample or to rotate the container so as to apply a centrifugal force to a sample deposited in the chamber and separate at least a first component and a second component of the deposited sample; and b) a sample separation unit coupled to the output port of the sample processing unit, the cell separation unit comprising separation column holder (42), a pump (64) and a plurality of valves (1-11) configured to at least partially control fluid flow through a fluid circuitry and a separation column (40) positioned in the holder, the separation column configured to separate labeled and unlabeled components of sample flowed through the column.

A culture flask has a flask body. The flask body has two transparent planes, one opening end, one recess end and at least one collecting recess. Both transparent planes are located on the opposite ends of the flask body. A collecting recess is formed on the inner surface of the recess end, while the cross sectional area of the recess end gradually decreases toward the bottom of the collecting recess. By forming a collecting recess with decreased cross sectional area, culture cells can be collected inside the bottom after centrifugation, and therefore the user no longer has to transfer the culture cells and culture medium to a centrifuge tube when replacing culture medium. Consumption of suction tube and centrifuge tube can be avoided, the time to replace the culture medium is reduced, and also the risk of contamination when replacing the culture medium is reduced.

The invention relates to a system, comprising: a) a sample processing unit, comprising an input port and an output port coupled to a rotating container having at least one sample chamber, the sample processing unit configured provide a first processing step to a sample or to rotate the container so as to apply a centrifugal force to a sample deposited in the chamber and separate at least a first component and a second component of the deposited sample; and b) a sample separation unit coupled to the output port of the sample processing unit, the cell separation unit comprising separation column holder (42), a pump (64) and a plurality of valves (1-11) configured to at least partially control fluid flow through a fluid circuitry and a separation column (40) positioned in the holder, the separation column configured to separate labeled and unlabeled components of sample flowed through the column.

The present disclosure relates to hollow fiber tangential flow filters, including hollow fiber tangential flow depth filters, for various applications, including bioprocessing and pharmaceutical applications, systems employing such filters, and methods of filtration using the same.

The present invention relates to methods for the specific separation of target cells from a biological sample, comprising specific binding of the target cells to phase-change hydrogel compositions and separation of respective cell-hydrogel complexes by counter-current centrifugation.

The present disclosure relates to hollow fiber tangential flow filters, including hollow fiber tangential flow depth filters, for various applications, including bioprocessing and pharmaceutical applications, systems employing such filters, and methods of filtration using the same.

A closed, automated and scalable stirred tank bioreactor platform, capable of sustaining high fold expansion of hPSCs is provided. hPSCs are expanded in a controlled bioreactor using perfused xeno-free media. Cell harvest and concentration are performed in closed steps. The hPSCs can be cryopreserved to generate a bank of cells or further processed as needed. Cryopreserved cells can be thawed into a 2D tissue culture platform or a 3D bioreactor to initiate a new expansion phase or be differentiated to the clinically relevant cell type. The expanded hPSCs express hPSC-specific markers, have a normal karyotype and the ability to differentiate to the cells of the three germ layers. This end-to-end platform allows large expansion of high quality hPSCs that can support the required cell demand for various clinical indications.