A method and apparatus for separating cell suspension into centrate and concentrate includes a solid wall rotatable centrifuge bowl (82). A single use rotatable core (88) is positioned in the bowl and bounds a cavity (90) which serves as a separation chamber. The bowl rotates about an axis (84). An inlet tube (92) and an outlet tube (102) extend along the axis in the bowl cavity. The centripetal pump (98) in the cavity is in connection with the outlet tube. The centrate discharge pump (120) is in connection with the outlet tube. A pressure damping reservoir (122) is fluidly connected between the outlet tube and the discharge pump. At least one control circuit (142) is operative to control the apparatus to maintain a positive pressure above atmospheric pressure within the cavity, so as to maintain the cell suspension away from at least one seal (106) that bounds the interior of the cavity.

A portable apparatus for collection and processing of human biological material containing adipose, such as extracted during a lipoplasty procedure, is useful for multi-step processing to prepare a concentrated product (e.g., stromal vascular fraction) or a fat graft composition. The apparatus has a container with an internal containment volume including an available processing volume in a range of from 75 cubic centimeters to 1300 cubic centimeters, and with a tissue retention volume and a filtrate volume separated by a filter and with a tapered portion to a collection volume for collecting concentrate product. The apparatus has a mixing device for mixing contents within the tissue retention volume, and including a rotatable shaft extending from outside of the internal containment volume to inside of the internal containment volume and comprises at least one mixing member that is disposed in the tissue retention volume and is connected with the shaft to move through the tissue retention volume relative to the filter when the shaft is rotated. Methods include use of the apparatus to process human biological material containing adipose, such as to prepare a stromal vascular fraction concentrate product or a fat graft composition.

Disclosed are devices for propagating at least one microtissue, methods for manufacturing such devices, and the use us such devices, said devices comprise at least one well, wherein said at least one well comprises an open upper section comprising an open upper end and an open lower end, and a lower section comprising an open upper end and a bottom end, wherein the upper section and the lower section are in fluid communication, and wherein the bottom end of the lower section of at least one well of said plurality of wells is provided with a well bottom, said lower section of said at least one well has a smaller cross-sectional area than the open upper section of the same well.

A method of killing specific cells from among a group of cells cultured in a culture vessel by quick and brief laser treatment, the cell culture vessel comprising a main body and a to-be-irradiated layer attached to the main body, the to-be-irradiated layer containing an ingredient capable of absorbing laser light upon laser irradiation, the group of cells being cultured on the surface of the to-be-irradiated layer, the method comprising: applying laser light to a partial area of the to-be-irradiated layer directly below the specific cells.

The present disclosure relates to a method for attaching, detecting and retrieving a single cell on a manipulation medium comprising the steps of: attaching the single cell onto the manipulation medium by applying a carrier liquid; partially drying out the carrier liquid; detecting a property of the single cell by rotating the manipulation medium and scanning a light beam over the manipulation medium; loosening the single cell from the manipulation medium without damaging the single cell by manipulating the single cell with an instrument; and retrieving the single cell from the manipulation medium by aspirating the single cell into a tube, wherein the carrier liquid is dried out to a degree corresponding to that the single cell does not move during the rotation of the manipulation medium and that the single cell can be loosened by the instrument without damaging the single cell. The disclosure further relates to an apparatus for detection and manipulation of one or more object(s),

A centrifuge layering insert configured for insertion into a centrifuge tube includes a tube having a first open end and a second open end. The centrifuge layering insert also includes a casing having an outer wall, an inner wall, a first open end, and a second substantially closed end. The casing forms an annular space around the tube between the tube first end and the tube second end. The tube has at least a portion of the length extending beyond the first open end of the casing in a direction opposite the second open end of the tube.

A portable apparatus for collection and processing of human biological material containing adipose, such as extracted during a lipoplasty procedure, is useful for multi-step processing to prepare a concentrated product (e.g., stromal vascular fraction) or a fat graft composition. The apparatus has a container with an internal containment volume with a tissue retention volume and a filtrate volume separated by a filter and with a tapered portion to a collection volume for collecting concentrate product. The apparatus has fluid suction features including a suction port and a conduit providing fluid communication from the suction port to a location within the filtrate volume within a tapered portion of the internal containment volume, and optionally including a second suction port and a second conduit to a different location in the filtrate volume with the second conduit being configured to permit elevation adjustment within the filtrate volume. Methods include use of the fluid suction features during collection of human biological material and/or post collection.

An assembly includes an adjustable dip tube that can selectively withdraw an isolated component at various heights within a vessel. The adjustable nature of the dip tube can improve the cell recovery rate as compared to conventional cell recovery assemblies. Additionally, the assembly can take advantage of the adjustable nature of the dip tube while maintaining an aseptic interior cavity of the assembly.

An autologous cell concentrating system and method are disclosed. The system has a blood separation component, a first vessel, a second vessel, a first valve, a second valve, and a concentration and flow logic and control component. The concentration and flow logic and control component is configured to: determine a first volume of a target cell-poor fraction in the first vessel to mix with a target cell-rich fraction in the second vessel in order to form a target cell-rich concentrate having a concentration of target cells that is within a target concentration range; and control the second valve to transfer the first volume of the target cell-rich fraction from the first vessel to the second vessel to form the target cell-rich concentrate. The target concentration range is between 1.0 and 1.510.sup.6 target platelets/L.

Manufacturing system and method for creating multiple tissue constructs from cells. System can include a thaw subsystem (if the cells are provided in a frozen state), an expansion subsystem, a concentration subsystem, and a tissue maturation subsystem. Each of these subsystems is modular and can be reconfigured, and the process can be repeated depending on the specific tissue process being implemented. Multiple tissue types can be combined in multiple bioreactors. The activities of multiple bioreactors can be coordinated and controlled in an automated manner by a supervisor controller. The supervisor controller can receive user input at the start of the process, and can manage the process henceforth, alerting the user if user actions are required.