A separation container for extracting a portion of a sample for use or testing and method for preparing samples for downstream use or testing are provided. The separation container may include a body defining an internal chamber. The body may define an opening, and the body may be configured to receive the sample within the internal chamber. The separation container may further include a seal disposed across the opening, such that the seal may be configured to seal the opening of the body, and a plunger movably disposed at least partially inside the internal chamber. The plunger may be configured to be actuated to open the seal and express the portion of the sample.

Methods of recovering cannabinoids from cell cultures include methods comprising steps of separating the cell culture at a temperature above the melting point of the cannabinoid to separate a light phase comprising liquid state cannabinoid from a heavy phase; and methods comprising treating the cell culture at a temperature below the melting point of the cannabinoid to separate a light phase from a heavy phase comprising solid state cannabinoid. Other methods include contacting the culture with a water-miscible solvent to form a water-miscible phase and an aqueous phase, separating the two phases and recovering the cannabinoid. Other methods include contacting the culture with a water-immiscible solvent to form a water-immiscible phase and an aqueous phase, separating the two phases, and recovering the cannabinoid. Other methods include washing the inner surface of a fermentation vessel with alkaline solution to recover cannabinoid attached to the vessel surface. Various methods make use of aqueous solvent systems comprising no organic solvent, aqueous solvent systems comprising added water-miscible organic solvent, and dual-phase aqueous/water-immiscible solvent systems.

This method for producing a cell cluster group comprises: a step for putting, into a well, a cell suspension obtained by suspending dispersed cells in a medium, using a cell incubator which includes the well and two or more recesses formed in the bottom of the well and in which the area of an opening of each recess in plan view is at most 1 mm.sup.2; a step for centrifuging the cell incubator; and a step for culturing the dispersed cells in the recesses.

A centrifuge rotor having a curved shape is offset on a spinning rotor base and creates contiguous areas of low to high centrifugal force depending on the distances from the axis of the rotor base and a method of separating components in a fluid based upon a difference in density of the components, the method comprising the steps of providing to a rotor as described herein the fluid containing the mixed together components to be separated based upon the difference in density of the mixed together components; continuously flowing the components in the fluid to the rotor through an input tube connected to the input port while the rotor is spinning about a centrifugal axis of rotation; separating the components in the fluid into fractions based upon the difference in density of the mixed together components with the use of centrifugal force when the rotor is spinning; collecting components having i) a first density via a first tube connected to the output port at the first end on the rotor, ii) a second density via a second tube connected to the output port at the second end on the rotor, iii) a third density via a third tube connected to the output port at the junction on the rotor and iv) a fourth density via a fourth tube connected to the output port between the input port and the output port at the first end.

Provided are a perfusion culture apparatus which can be utilized in long-duration culturing and which has a simple mechanism and is easy to use; and a centrifugal separator. The perfusion culture apparatus has a centrifugal separation tank 30 for centrifugally separating a culture solution W delivered from a culture tank 10, and a discharge mechanism 60 for causing a centrifugally separated supernatant of the culture solution to be discharged from the centrifugal separation tank. The centrifugal separation tank is provided with a cylindrical inner wall surface 32, and is configured so as to rotate about the center axis of the inner wall surface and thereby centrifugally separate the culture solution. The discharge mechanism includes a suction pipe 62 provided with a first end portion 64 which faces the inner wall surface and is disposed closer to the center axis than the culture solution during centrifugal separation, and a second end portion 66 which is disposed outside the centrifugal separation tank. In a state in which the centrifugal separation tank is rotated and the culture solution is centrifugally separated, the air in the centrifugal separation tank is sucked from the first end portion of the suction pipe, and the centrifugally separated supernatant of the culture solution is thereby discharged, together with the air, from the second end portion.

The present disclosure relates generally to a tubing apparatus for separating and concentrating stem and stromal cells, also known as regenerative cells, from adipose tissue, more specifically to a defined process of extracting, separating and concentrating clinically useful regenerative cells from adipose tissue using a combination of mechanical disruption and filtration-centrifugation to obtain a highly enriched heterogeneous population of stem stromal cells. The centrifuge tube comprising a threaded top cap with male luer access port to be adapted to female luer of a syringe, a tapered main tubular barrel, a thin disk filter, and a bottom conical cap with luer access port for withdrawal of stem stromal cells via a syringe.

A culture flask has a flask body. The flask body has two translucent planes, one opening end, one recess end and at least one collecting recess. Both translucent planes are located on the opposite ends of the flask body. A collecting recess is formed on the inner surface of the recess end, while the cross sectional area of the recess end gradually decreases toward the bottom of the collecting recess. By forming a collecting recess with decreased cross sectional area, culture cells can be collected inside the bottom after centrifugation, and therefore the user no longer has to transfer the culture cells and culture medium to a centrifuge tube when replacing culture medium. Consumption of suction tube and centrifuge tube can be avoided, the time to replace the culture medium is reduced, and also the risk of contamination when replacing the culture medium is reduced.

The present invention relates to methods for the specific separation of target cells from a biological sample, comprising specific binding of the target cells to phase-change hydrogel compositions and separation of respective cell-hydrogel complexes by counter-current centrifugation.

A stirring device 11 includes a stirring mechanism 7 and a control unit 15. The stirring mechanism 7 includes a motor 74 and a pedestal portion 77. In the stirring device 11, the motor 74 repeats forward rotation and reverse rotation alternately under the control of the control unit 15. Then, the pedestal portion 77 repeats turning in one direction and turning in the other direction alternately. As a result, the test tube S repeats the movement in one direction and the movement in the other direction alternately, and the vortex rotating in one direction and the vortex rotating in the other direction are alternately generated in the mixed solution in the test tube S. Then, the mixed solution in the test tube S is stirred to be rotated, and is stirred to be moved in the vertical direction. Therefore, the mixed solution in the test tube S can be efficiently stirred. As a result, the time required for stirring the mixed solution in the test tube S can be shortened.

When a stirring device 11 performs processing of stirring a mixed solution in a test tube S, a holding mechanism 13 and a movement mechanism 14 are operated so that the test tube S is moved to a position of B2. Then, in the test tube S, the cells attached to the inner surface of the test tube S and the liquid are separated. Further, the cells attached to the inner surface of the test tube S are detected by a color sensor 152. Therefore, it is possible to reduce the work for determining the end of the stirring processing by the user. As a result, the workability of the user when using the stirring device 11 can be improved.