The invention relates to a method for producing microalgae on a support (14) that is movably mounted essentially in an aqueous medium contained in a tank (12), said method comprising a succession of phases in which the microalgae developing on the support (14) are exposed to sunlight and phases in the shade, the light intensity received in the shade being less than 50% of the average light intensity received during the sunlight exposure phases. The total length of the shade phases is more than 50% longer than the total length of the sunlight exposure phases.

The present disclosure teaches an algal growth system. The algal growth system includes a flexible sheet material, a liquid reservoir, a mechanism to rotate the flexible sheet material, a resource reclamation device, and an enclosure. The flexible sheet material facilitates growth of a biofilm. The liquid reservoir is configured to retain a contacting liquid in contact with the flexible sheet material and the mechanism rotates the flexible sheet material through the contacting liquid. The resource reclamation device associated with the enclosure is configured to collect and provide a resource for reuse.

The present disclosure teaches an algal growth system. The algal growth system includes a flexible sheet material, a liquid reservoir, a mechanism to rotate the flexible sheet material, a resource reclamation device, and an enclosure. The flexible sheet material facilitates growth of a biofilm. The liquid reservoir is configured to retain a contacting liquid in contact with the flexible sheet material and the mechanism rotates the flexible sheet material through the contacting liquid. The resource reclamation device associated with the enclosure is configured to collect and provide a resource for reuse.

Provided is a cell detachment system including: a scraper having magnetism, for detaching cells adhering to a culture surface; a magnetic field applying unit configured to apply a magnetic field to the scraper; and a scraper position control unit configured to control a position of the scraper by controlling the magnetic field applying unit, wherein the scraper is configured to move while rotating under a state in which no magnetic field is applied thereto, and under a state in which a magnetic field is applied thereto by the magnetic field applying unit, to be caused to move, without rotating, by the scraper position control unit so as to detach the adhering cells.

In a cell culture in a sealed cell culture container, gradual deterioration of culture environment is prevented. A cell culture method in which cell culture is conducted by adding a fresh culture medium to a culture container in which cells and a culture medium are enclosed, which includes the steps of adjusting the pH of said culture medium to be added to be higher than the pH which is optimum for culture of the cells, and/or adjusting the partial pressure of dissolved carbon dioxide of the culture medium to be added to be lower than the pressure which is optimum for culture of the cells, and/or adjusting the partial pressure of dissolved oxygen of the culture medium to be added to be higher than the pressure which is optimum for culture of the cells; and mixing the adjusted culture medium and the culture medium in said culture-container to adjust at least any of the pH, the partial pressure of dissolved carbon dioxide and the partial pressure of dissolved oxygen to be optimum for culture of said cells, thereby to culture the cells in the culture container.

Adhesive culture cells cultured by using a culture container made of a flexible film can selectively be released from the culture container without causing damage to the cells. A cell release method for releasing, from a culture container, adhesive culture cells cultured adhering to the culture container made of a flexible film includes bringing a surface of the film of the culture container into contact with the adhesive culture cells, applying a pressure to the adhesive culture cells, and releasing the adhesive culture cells from the culture container, wherein the surface of the film faces a surface of the film of the culture container to which the adhesive culture cells adhere.

A method for collecting cells that have been cultured by using a cell culture kit in which, at least, a culture container for cultivating cells, a cell collection container for collecting cells after cultivation and a waste liquid container for collecting a culture medium after cultivation are linked to one another through a conduit is provided. The method includes transferring a culture supernatant that has been cultured from the culture container to the waste liquid container, transferring a concentrated suspension of cells from the culture container to the cell collection container, returning part of the culture supernatant discharged in the waste liquid container to the culture container and allowing cells remaining in the culture container to be suspended in a suspension of cells, and transferring again a suspension of cells in which remaining cells are suspended from the culture container to the cell collection container.

A full-automatic microorganism detecting enrichment system and the enrichment method thereof are provided. A transmission apparatus is controlled by a industrial control computer to transmit a to-be-detected sample placed on a preset operating position, a culture medium comprising culture, a pipeline filter specially for collecting bacteria and a filter head sequentially into a sterilization cabin, an middle package removing cabin, an inner package removing cabin, an enrichment operating cabin, a buffer cabin and a positive bacteria filling cabin to perform corresponding operations and enrich the microorganism in the to-be-detected samples. Said system can achieve a fully automated enrichment of the microorganism in the to-be-detected samples, needs no manual operation, and saves time and labor force. Further, it can avoid false positive or false negative result caused by an effect of human factor, and achieve sterile and automatic operation with an accurate detection result.

A sampler for use with a bioreactor for growing a cell culture is disclosed. In one embodiment, the bioreactor includes a structured fixed bed including a removable sample portion for recovering a sample of cells from the cell culture. Related apparatuses and methods are also disclosed.

The present disclosure teaches an algal growth system. The algal growth system includes a flexible sheet material, a liquid reservoir, a mechanism to rotate the flexible sheet material, a resource reclamation device, and an enclosure. The flexible sheet material facilitates growth of a biofilm. The liquid reservoir is configured to retain a contacting liquid in contact with the flexible sheet material and the mechanism rotates the flexible sheet material through the contacting liquid. The resource reclamation device associated with the enclosure is configured to collect and provide a resource for reuse.