Separating apparatus, comprising a sedimentation settler and a collection vessel disposed underneath and being in fluid communication with the sedimentation settler, the collection vessel forming a receiving chamber having an outlet at or adjacent to the chamber bottom and having an inlet opening, wherein the collection vessel is arranged such the flow direction of the fluid in the area underneath the sedimentation settler is substantially in line with the direction of the channels of the sedimentation settler.

The present invention is a system for optimizing production of biomass. The system contains one or more growth modules under a shading element that diffuses light. Each of the growth modules has a plurality of vertical growth columns arranged on a bottom manifold. The biomass is grown within a liquid growth medium held within each of the vertical growth columns until it reaches a desired growth. The biomass is then harvested through gravitational flow of the biomass and the liquid growth medium out of the bottom of each growth column. The bottom manifold collects gravitational flow of the biomass and liquid growth media from each growth column and aggregates the output of each individual column into a collective output of the growth module.

The present set of embodiments relate to a system, method, and apparatus for separating cells from a suspension using a gravity driven cell separation device. The apparatus may include a chamber that is separated into a gas compartment and a liquid compartment allowing for transfer of gas through a membrane into the liquid compartment where the cell suspension flows during operation. The embodiments are designed to maximize the gravitational separation effect on the suspension while mitigating the loss of surface area through which gas transfer can occur through a membrane to provide nutrients to cells in the suspension by tilting the cell separation device on two or more axes.

A closed cell separation operation apparatus includes a first tube body having a vent and an inlet, a second tube body having a vent and an inlet and a connecting pipe, connecting the first tube body and a second tube body; the vent of the first tube body allows air to enter and exit the first tube body and the inlet of the first body allows a fluid from an external fluid source to enter the first tube body; and the first tube body having an outlet; the vent of the second tube body allows air to enter and exit the second tube body and the inlet of the second tube body allows at least one part of the fluid from the outlet of the first tube body to enter the second tube body and the second tube body having an outlet. The closed cell separation operation apparatus provides a closed, contamination-free environment for both centrifugation and post-centrifugation supernatant separation.

The present invention relates to a cell culture device comprising at least a fluidic channel having at least a cell medium inlet and a cell medium outlet, said fluidic channel comprising a lower wall extending between the cell medium inlet and the cell medium outlet, the cell culture device further comprising at least one recess configured to receive a plurality of cells, said recess being formed by the lower wall and defining a bottom surface, and at least a cell trap, said cell trap being configured to capture a cell advected in the fluidic channel and then to make the cell sediment to the bottom surface of the recess.

The embodiments of the present application relate to a photosynthetic bioreactor system in the field of bioreactors. The system includes at least one bioreactor with a transparent cultivation region for photosynthetic microorganisms; a conditioning device for producing ideal liquid culture medium; a collection device for recovering organic matter from the bioreactor; and a nano-bubble generating device for converting carbon dioxide-containing gas into minute bubbles for liquid introduction. The bioreactor includes a culture medium inlet, a culture medium outlet, and a gas inlet communicating with the transparent cultivation region. The conditioning device connects to each bioreactor's culture medium inlet, the collection device connects to each culture medium outlet, and the nano-bubble generating device connects to each air inlet. The transparent cultivation region has a thickness less than a preset dimension to enable light penetration.

The present invention relates to methods and systems for performing perfusion cell culture whereby the supernatant of the bleed stream is recovered.

Provided is a cell culture method including introducing a factor into cells in a cell culture vessel, and culturing the cells into which the factor has been introduced in the same cell culture vessel. Also provided is a mononuclear cell collection method including treating blood to prepare a treated blood from which erythrocytes have been at least partially removed, diluting the treated blood, causing sedimentation of mononuclear cells contained in the diluted treated blood, removing the supernatant from the diluted treated blood, and collecting the mononuclear cells.

A 3D multi-organ co-culture chip is provided by present disclosure and comprises a chip body, wherein one or more groups of culture modules are arranged on the chip body; an each group of the culture modules comprises a fluid storage hole of which an end is open and is positioned at an upper surface of the chip body; a first culture micropore which is positioned below a corresponding fluid storage hole and communicates with the corresponding fluid storage hole; a second culture micropore which is positioned below a corresponding first culture micropore and communicates with the corresponding first culture micropore; and a plurality of second fluid operation holes, wherein an end of an second fluid operation hole is open and is positioned on the upper surface of the chip body and an other end of the second fluid operation hole communicates with a corresponding second culture micropore through a channel.

A method for treating an aqueous fluid comprising a biodegradable organic substance in an installation comprising an upflow bioreactor containing a sludge bed, said sludge bed comprising biomass, an external separator, and a conditioning tank, the method comprising: treating the fluid in the conditioning tank; feeding the treated fluid into a lower part of the bioreactor and forming biogas; withdrawing the fluid from an upper part of the bioreactor, which withdrawn fluid comprises biomass; feeding the aqueous fluid withdrawn from the upper part of the bioreactor into the external separator wherein the aqueous fluid comprising the biomass is separated into a liquid phase, and a fluid phase enriched in biomass; returning said fluid phase enriched in biomass from the external separator to the bioreactor; and returning a part of said liquid phase to the conditioning tank.