There is provided a cell culture vessel for culturing a cell in an interior thereof, wherein a width of a bottom surface is equal to or larger than a height of a side surface, the cell culture vessel comprising a flow path configured to supply a fluid into the interior, and wherein the interior is able to be closed.

A biological component concentration fluid assembly includes magnetizing microparticles that are surface-activated to bind with (or are bound to) a biological component; a multi-fluid density gradient column with a first fluid layer, a second fluid layer, and a third fluid layer; and a magnet to attract and draw the magnetizing microparticles from the first fluid layer, through the second fluid layer, and into the third fluid layer. The first fluid layer has a first fluid density, and a second fluid layer has a second fluid density that is greater than the first fluid density, and is positioned beneath the first fluid layer. A third fluid layer has a third fluid density that is greater than the second fluid density and is positioned beneath the second fluid layer. The second and third fluid layers in this example are formulated to interact with the surface of the magnetizing microparticles.

The present disclosure includes a method of forming and loading a multi-fluid density gradient column. The method can include forming a multi-fluid density gradient column and loading magnetizing microparticles into a first fluid layer or a second fluid layer of the multi-fluid density gradient column. Forming the multi-fluid density gradient column can include loading a first fluid having a first fluid density in a multi-fluid density gradient column to form a first fluid layer and loading a second fluid having a second fluid density greater than the first fluid density in the multi-fluid density gradient column to form a second fluid layer. The multi-fluid density gradient column can be fluidly coupled to a fluid processing device. The magnetizing microparticles can be surface-activated to bind with a biological component or can be bound to the biological component.

A 3D multi-organ co-culture chip is provided by present disclosure and comprises a chip body, wherein one or more groups of culture modules are arranged on the chip body; an each group of the culture modules comprises a fluid storage hole of which an end is open and is positioned at an upper surface of the chip body; a first culture micropore which is positioned below a corresponding fluid storage hole and communicates with the corresponding fluid storage hole; a second culture micropore which is positioned below a corresponding first culture micropore and communicates with the corresponding first culture micropore; and a plurality of second fluid operation holes, wherein an end of an second fluid operation hole is open and is positioned on the upper surface of the chip body and an other end of the second fluid operation hole communicates with a corresponding second culture micropore through a channel.

Spent stillage remaining after the fermentation of a feedstock for ethanol production may be processed to recover, use, and/or recycle the constituent components of the stillage. Stillage may be mixed, heated, and held at a desired temperature for a period of time. The stillage may then be cooled and treated with an enzyme. The enzymatically treated stillage may be emulsified with oil and water, and then permitted to settle into discrete layers. Individual layers may then be processed.

The present disclosure relates to settling devices for separating particles from a bulk fluid with applications in numerous fields. The particle settling devices of the present disclosure may include a stack of truncoconical cones that may be arranged in opposite orientation, apex to base. Other embodiments include several concentric vertical tubes attached to conical surfaces at the bottom, with inclined settling strips attached to the vertical tubes in annular regions between the tubes. These devices are useful for separating small (millimeter or micron sized) particles from a bulk fluid with applications in numerous fields, such as biological (microbial, mammalian, plant, insect or algal) cell cultures, solid catalyst particle separation from a liquid or gas and waste water treatment.

A system for isolating a target molecule from a bioprocess fluid includes a single-use disposable separation device having a plurality of perimeter-bonded layers defining one or more mesofluidic channels of the separation device, wherein each layer includes a biocompatible polymer material, wherein the separation device is configured to separate at least a portion of particles from the bioprocess fluid to generate a substantially clarified bioprocess fluid, and a chromatography system fluidically coupled at the outflow of the separation device in a configuration for further processing the clarified bioprocess fluid.

A method includes containing an algae slurry within a cultivation vessel, introducing the algae slurry into a dissolved air floatation (DAF) system, and operating the DAF system in a low-recovery mode and thereby selectively removing impurities from the algae slurry. Algal biomass is then harvested from the algae slurry after removing the impurities from the algae slurry.

Specialized culture plates for imaging cells in a quick, high throughput manner are provided. Ideally the wells of the culture plate have triangular, square, or V-shaped wells or cell sorting walls having a plurality of vertices, and more complicated variations thereof are also possible. The plates are tilted or rotated to collect the cells at the vertex or vertices of the wells, optionally vibrated to speed the collection, then the vibration and tilt or rotation removed for some period of time, whereon the cells are imaged through the flat transparent bottom of the plate.

A bioprocessing method for cell therapy includes the steps of genetically modifying a population of cells in a bioreactor vessel to produce a population of genetically modified cells, and expanding the population of genetically modified cells within the bioreactor vessel to generate a number of genetically modified cells sufficient for one or more doses for use in a cell therapy treatment without removing the population of genetically modified cells from the bioreactor vessel. Cells are settled on a gas permeable, liquid impermeable membrane for expansion, and are resuspended when, or after, the desired cell density is reached.