An airlift bioreactor for perfusion cell culture comprises a single- or multi-tiered riser for working volume scale-up, an internal inclined gravity settler for cell retention, an outlet port for cell-free spent culture medium removal, and an inlet port for fresh medium addition.

An anaerobic reactor (1) for treating waste water includes a reaction vessel (2) and a three phase separator (4) above the reaction vessel and arranged to receive effluent from the reaction vessel. The three phase separator includes an outer wall (10, 14) connected at its bottom to the top of the reaction vessel and a liquid outlet (42), a lid (16) closing the top of the outer wall. The lid has a gas outlet (17) above the level of the liquid outlet. The three phase separator also includes a funnel (18) arranged above the reaction vessel, a guide wall (30) spaced from and arranged radially outward of the funnel so to surround an upper aperture of the funnel and a baffle wall (36) spaced from and arranged between the guide wall and the liquid outlet.

There are provided methods for the production of lipids such as sophorolipids. Also provided are apparatus for use in the production.

A method for producing hydrogen from organic material. Organic material and hydrogen-producing microorganisms are provided in a completely mixed bioreactor for breaking down the organic material into H.sub.2, CO.sub.2, fatty acids, and alcohols. H.sub.2, CO.sub.2, and a first liquid effluent are recovered from the completely mixed bioreactor. The first liquid effluent includes hydrogen-producing microorganisms, fatty acids, and alcohols. The first liquid effluent is provided into a gravity settler for separating the first liquid effluent into a concentrated biomass (including hydrogen-producing microorganisms) and a second liquid effluent (including at least a portion of the fatty acids and the alcohols). The concentrated biomass is provided into the completely mixed bioreactor. An input voltage is applied to at least one of the completely mixed bioreactor and the gravity settler for facilitating an electrohydrogenesis process therein.

The present disclosure relates to settling devices for separating particles from a bulk fluid with applications in numerous fields. The particle settling devices of the present disclosure may include a stack of truncoconical cones that may be arranged in opposite orientation, apex to base. Other embodiments include several concentric vertical tubes attached to conical surfaces at the bottom, with inclined settling strips attached to the vertical tubes in annular regions between the tubes. These devices are useful for separating small (millimeter or micron sized) particles from a bulk fluid with applications in numerous fields, such as biological (microbial, mammalian, plant, insect or algal) cell cultures, solid catalyst particle separation from a liquid or gas and waste water treatment.

A cell culture apparatus includes a culture tank having an inlet and an outlet, a culture-medium supplying unit that supplies a culture medium to the culture tank through the inlet, and a culture-medium discharging unit that discharges the culture medium through the outlet. The culture tank includes a division wall that can divide the internal space in a direction of gravity into an upper space and a lower space. The outlet opens into the upper space, and the division wall enables switching between a state where the internal space of the culture tank is divided into the upper space and the lower space and a state where the upper space and the lower space are in communication with each other.

The invention generally provides a device and a method for isolating cells from tissues and using same in vivo or in vitro.

The present disclosure relates to a system for washing lipoaspirate, comprising a chamber; a lid connecting to the top side of the chamber and comprising a harvest port configured to connect to and receive a harvested lipoaspirate from a hose; a sub-assembly comprising a mesh basket and disposed in an upper portion of the chamber, the sub-assembly configured to drain the lipoaspirate through the mesh basket; a tray located in a lower portion of the chamber and comprising a foam assembly configured to concentrate a drained lipoaspirate; and an extraction port along a side wall of the chamber and configured to permit extraction of a concentrated lipoaspirate from the system.

A bioreactor for use on a moving platform including a bag. According to a first aspect, the bag defines a three-dimensional geometrical shape including at least one lower collecting vertex in which at least two edges of the geometrical shape meet at an angle not greater than 90?. The bioreactor further includes at least one port or sensor arranged in the vicinity of the collecting vertex. According to a second aspect, the bag defines a two-dimensional geometrical shape or a three-dimensional geometrical shape. The geometrical shape includes at least one edge, in which at least two faces of the geometrical shape meet at an angle not greater than 90?. A longitudinal port/sensor element is arranged along the edge and includes several ports and/or sensors. A bioreactor motion system including a moving platform and a bioreactor according to the first or second aspect. A method of performing a bioprocess.

A method of separating waste material to a plurality of separated materials is disclosed herein. The method is effected by subjecting the waste material to a separation according to specific gravity, to thereby obtain two or more fractions, and optionally subjecting one or more of said fractions to additional separation procedures, to thereby obtain two or more of a low-density polymeric material, a high-density polymeric material, a metal, a glass, an oil, and lignocelluloses. The disclosed method can further be effected by processing one or more of the separated materials to thereby obtain one or more processed materials of a beneficial use. Further disclose herein are separated and/or processed materials obtainable by the method, articles-of-manufacturing comprising same, and systems for separating and/or processing the waste material.