A series of multi-dimensional acoustic standing waves is set up inside a growth volume of a bioreactor. The acoustic standing waves are used to hold a cell culture in place as a nutrient fluid stream flows through the cell culture. The nutrient fluid stream dislodges some cells from the cell culture, which can then be recovered for cell therapy applications. The cell culture continues to expand and reproduce, permitting continuous recovery of cells from the bioreactor.

A device for analysis of cells comprises: an integrated circuit arrangement on a substrate; a dielectric layer formed above the integrated circuit arrangement; a microelectrode array layer formed above the dielectric layer, said microelectrode array layer comprising a plurality of individual electrodes, wherein each electrode is connected to the integrated circuit arrangement through a via in the dielectric layer; and wherein a plurality of longitudinal trenches in the dielectric layer and the microelectrode array layer are for stimulating cell growth on a surface of the device

The present disclosure relates to a multi-chamber bioreactor, preferably in a polymeric material with a 3D structure, adapted for cell-mono and co-culture, with at least two entries and outputs of culture medium adaptable to be used as a static culture system and to incorporate a dynamic platform creating a bioreactor. The disclosure also relates to a technique based on a bioreactor device that allows the creation of two or more different tissues integrated with the natural phenotype, using an integrated and continuous 3D support structure.

The present application provides a method for lysis or electroporation of cells in a biological sample including the following steps: passing cells of the sample, suspended in a fluid, through a flow path with a preset flow speed, wherein the flow path runs through a detection apparatus for detecting individual cells and wherein the flow path includes at least two electrodes for generating an electric field, which electrodes are located downstream of the detection apparatus and which electrodes are coated with a dielectric material with a relative permittivity greater than 3.9, wherein the coaling at least covers the surface of the electrodes that faces the flow path, and when the presence of a specific cell is detected in the detection apparatus, then an electric field is generated between the electrodes when the detected cell passes between the electrodes in dependence of the flow speed, wherein the electric field causes electroporation or lysis of the cell.

A method of targeted electroporation and/or lysis of eukaryotic cellular bodies in a biological sample with at least two subgroups of eukaryotic cellular bodies, wherein each subgroup has a different susceptibility to electroporation and/or lysis in electric fields, including the following steps: transferring the biological sample in a chamber, exposing the biological sample to an electric field in the chamber, wherein the electric field is generated by at least two electrodes which are coated with a dielectric material with a relative permittivity greater than 3.9, and selecting the electric parameters of the electric field such as the field strength, the frequency or the wave form so that the subgroups are differently affected by said electric field for electroporation and/or lysis; as well as devices for the method.

A system, device and method for electroporation of living cells and the introduction of selected molecules into the cells utilizes a fluidic system where living cells and biologically active molecules flow through a channel that exposes them to electric fields, causing the molecules to be transferred across the cell membrane. The device is structured in a manner that allows precise control of the cells location, motion, and exposure to electric fields within the flow channel device. The method is particularly well suited for the introduction of DNA, RNA, drug compounds, and other biologically active molecules into living cells.

A method for accelerating the migration of cells by applying a time-varying magnetic field to induce eddy currents that promote electrotaxis (galvanotaxis) of cells. The method of the present invention accelerates the healing of wounds by electrotaxis of cells.

System (100) and method for processing biomass. The system comprises a combined heat and power plant (102), an interface (114) for feeding biogas to a traffic fuel production unit, interfaces (114) to a district heating system (106a) and an electrical grid (106b), and a hydrolysis device (108), a digestion device (110), a dryer (116) and a heat recovery unit (112), which are operatively coupled for transferring heat, intermediate products and final products of the process, wherein raw biomass is received into the Fuel hydrolysis device (108), biomass processed by the hydrolysis device (108) is fed to the digestion device (110), biogas obtained in the digestion device (110) is fed to the traffic fuel production unit (104), heat is recovered from the hydrolysis device (108), biomass processed by the digestion device (110) is dried by the heat recovered from the hydrolysis device (108), heat is recovered from the dryer (116), heat recovered from the dryer (116) is fed to the hydrolysis device (108) to be used in pre-heating of the received raw biomass, heat recovered from the dryer (116) is fed to the district heating (106a), and production of electricity is fueled by the dried biomass from the dryer (116).

A device for use in laser optical transfection of biological targets including an optofluidic microdevice and a piece of optical glass. The optofluidic microdevice has a central vertical outlet and a microchannel network that includes a plurality of entrapping channels with narrowings. The microchannel network is fused with the optical glass. In one aspect the device is used with a petri dish having an optical window. In another aspect the device is used with a well plate having a plurality of wells and associated optical windows. In a third aspect the device is used with a barrier. Each of the aspects forms a peripheral space around the optofluidic microdevice capable of retaining a live culture of biological targets and material that is desired to be injected into those biological targets. Polymer tubing is inserted into the central vertical outlet which connects the device to an external pump. The external pump provides an inward suction force which draws the biological targets from the peripheral space into the microchannel network. The biological targets are then captured at the openings or within the narrowings in the entrapping channels of the microchannel network where they can be transfected by laser light emitting from a laser through the optical glass.

The subject of the invention is a microprobe for selective electroporation comprising at least two metal electrodes (A) immersed in a glass rod (E), characterised in that the glass rod (E) made of a primary glass is 50 μm to 2 mm in diameter, preferably 50 μm to 500 μm, the metal electrodes (A) made of a metal alloy are formed as rods with diameter of 1 μm to 100 μm, preferably 20 μm to 30 μm, wherein endings of those rods are exposed, wherein the primary glass and the metal alloy are matched in such manner that dilatometric softening temperature DTM of the primary glass is highly similar to the temperature of melting for the metal alloy.
The invention also includes a method of manufacturing of such a microprobe.