Generation of energy and storage of energy for subsequent use is provided by electromethanogenesis of carbon dioxide into a fuel gas and the storage of the fuel gas for subsequent use. An electromethanogenic reactor includes an anode conductor and a cathode conductor wherein the cathode conductor includes submicron to micron scale pores. Electromethanogenesis microbes and/or enzymes are located in the micron scale pores of the cathode electrode conductor. Carbon dioxide is introduced into the electromethanogenic reactor and the electromethanogenesis microbes/enzymes and the carbon dioxide interact and produce a fuel gas. The fuel gas is stored for subsequent use, for example use in power generation.

To provide a technology for confirming that a desired particle is recovered in single cell analysis.

The present technology provides a particle confirming method including a correlating step of correlating ID information possessed by a particle trapped in a well in a particle trapping region with position information of the well, a discharging step of discharging the particle from the well, an ID information acquiring step of acquiring ID information of the particle after the discharging step, and a confirming step of confirming the position of the well in which the particle has been trapped, on the basis of the acquired ID information. In addition, the present technology also provides a particle trapping chip and a particle analyzing system to be used for carrying out the method.

In various embodiments, a Laser-actuated Supercritical Injector (LASI) is provided. This device provides high-speed fluidic jet injection into biological samples, such as cells, organs, and tissues (including skin). In certain embodiments the LASI devices exploit high-speed fluidic jets that are pushed by rapid bubble expansion in a fluid. The bubbles are formed when liquid confined in microcavities or holes are heated up to above the supercritical temperature of the fluid. This leads to the formation of a short but ultra-high vapor pressure (supercritical) fluid that ejects the fluid (and any cargo contained therein) out through microchannels. This jet penetrates a cell, organ or tissue juxtaposed to a surface containing the microchannels and the jet provide sufficient force to penetrate into the cell, tissue, or organ leading to effective deliver of a cargo.

A system, device and method for electroporation of living cells and the introduction of selected molecules into the cells utilizes a fluidic system where living cells and biologically active molecules flow through a channel that exposes them to electric fields, causing the molecules to be transferred across the cell membrane. The device is structured in a manner that allows precise control of the cells location, motion, and exposure to electric fields within the flow channel device. The method is particularly well suited for the introduction of DNA, RNA, drug compounds, and other biologically active molecules into living cells.

Example systems, apparatuses, methods, and computer program products are disclosed for electroporating cells in a tissue using a set of voltage pulses generated by capacitor charge circuitry based on a voltage supply. An example method includes continuously monitoring a set of characteristics of the voltage supply and the set of voltage pulses; generating a first set of monitor signals based on the set of characteristics; detecting a first fault condition based on the first set of monitor signals; and generating a first crowbar trigger activation signal. The example computer method further includes: detecting a second fault condition based on a second set of monitor signals generated based on the first set of monitor signals; and generating a second crowbar trigger activation signal. Subsequently, the example computer method includes electrically disconnecting the capacitor charge circuitry from electroporation electrode circuitry based on either the first or second crowbar trigger activation signal.

The purpose of the present invention is to provide a method which is designed to form an intracellular recording electrode for a cell by a simple operation which is less invasive to the cell and does not need a magnetic force, and with which the short-term or long-term intracellular potential can be accurately measured. More specifically, provided is a method comprising: securing, to a manipulator or the like, a holder provided on a conductor having a conductive nano structure at a tip; making the tip nano structure part penetrate a cell membrane while adjusting the amount of pressure applied to the target cell, thereby forming an intracellular recording electrode independently secured above the cell; and measuring the intracellular potential. The conductive nano structure at the tip and the conductor main body do not have to be magnetic but may be stuck together by magnetic force or may be formed as one body. When the cell membrane potential of a target cell cultured in a typical culture vessel is recorded, by forming the conductor main body of a magnetic electrode (MagEle) and independently securing same using a ring-shaped magnet that is provided on the lower surface of the culture vessel and that secures a light projection path or a light observation path through the center thereof, measurement of the intracellular potential of the target cell and fluorescent observation of changes in intracellular potential due to a light stimulus or intracellular calcium dynamics can be performed simultaneously.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

The present disclosure provides a flow-through electroporation device configured for use in an automated multi-module cell processing environment and configured to decrease cell processing time and the risk of clogging.

One or more light sources may apply stimuli to a colony of organisms. The stimuli may include visible and non-visible light. The stimuli, taken together, may tend to favor survival of organisms that are adapted to perform photosynthesis which involves absorbing energy from infrared or ultraviolet light. In some cases, the set of stimuli may include illuminating the entire colony of organisms with green light, illuminating only a first portion of the colony with pulsed ultraviolet light, and illuminating only a second portion of the colony with pulsed infrared light.

Systems and methods are provided for transfecting cells, such as mammalian cells and nonmammalian cells, using an electroporation apparatus having an electroporation chamber including an upper micromesh electrode, a lower micromesh electrode and a path defined in the electroporation chamber. The electroporation apparatus includes a first input allowing passage of cells into the electroporation chamber and a first output allowing passage of electroporated cells from the electroporation chamber. The first input and the first output are separated by an offset distance.