Devices and methods are presented for delivery of various macrostructures into cells, such as depleted cells as well as methods of generating engineered cells using said devices and methods. Cells are placed on a porous membrane. A force is applied by a configurable actuator to a deformable fluid reservoir that generates an applied pressure to macrostructures in a solution, causing the macrostructures to pass through the porous membrane and triggering uptake of at least some of the macrostructures into the cells to form transfected cells. Said devices and methods may be used in a process to replace defective endogenous mtDNA with corrected mtDNA to generate cellular-based therapeutics for administration to a patient. The customizable actuator may be configured with parameters to optimize efficiency for a given cell type and material to be transfected. Machine learning techniques also may be utilized to optimize transfection parameters.

A method and apparatus for improving transfection efficiency and/or an intra-cellular delivery process in one or more eukaryotic cells is provided. The method includes providing at least one naked agent suitable for transfection and/or intra-cellular delivery. Introducing the at least one naked agent to one or more eukaryotic cells to form a mixture or transfection mixture and allowing the mixture or transfection mixture to undergo a transfection process or intra-cellular delivery process to form one or more transfected or treated eukaryotic cells. The method includes directing pulsed electromagnetic signals provided at any or any combination of a pre-determined frequency, at a pre-determined pulse rate, or at a pre-determined power, at the at least one a naked agent at step (a) prior to creating the mixture or transfection mixture, at the mixture or transfection mixture in step (b), at the mixture or transfection mixture in step (c) and/or at the transfected or treated cell mixture after step (c).

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

A method and apparatus for improving transfection efficiency in eukaryotic cells including the steps of providing a transfection mixture including an agent associated with at least one amphiphilic construct suitable for transfection. Adding the transfection mixture to one or more eukaryotic cells to form a transfection complex and allowing the transfection complex to undergo a transfection process to form one or more transfected cells. The method also includes the step of directing pulsed electromagnetic signals provided at any or any combination of a pre-determined frequency, at a pre-determined pulse rate, or at a pre-determined power, at the transfection mixture at step (a) prior to creating the transfection complex, at the transfection complex in step (b), at the transfection complex in step (c) and/or at the transfected cell complex after step (c).

A well for electrically stimulating at least one cell. The well includes a bottom portion and comprises an adaptive electrode arrangement for introducing an electric field into the well. The adaptive electrode arrangement includes a pair of electrodes disposed within the well. Each electrode of the pair of electrodes has a distal end and is independently and axially displaceable relative to the other electrode and the bottom portion of the well. The distal end of each electrode of the pair of electrodes is in contact with the bottom portion of the well, ensuring a uniform and constant electric field is applied within the well.

The present disclosure is drawn to microfluidic cellular membrane modification. In one example, a method of modifying cells can include pumping a fluid comprising cells in a forward direction through a microfluidic channel, applying an electric field within the microfluidic channel as cells flow in the forward direction through the electric field and beyond within the microfluidic channel, and pumping the fluid in a backward direction through the microfluidic channel, wherein cells flow in the backward direction returning through the electric field.

The present disclosure provides cartridges, pump assemblies, and other systems, methods, and apparatuses for manufacture of a cell therapy. A rigid cartridge includes rigid walls defining a rigid housing. An interior surface of a first rigid wall includes a first mating mechanism to engage a second mating mechanism of a docking device to removably coupled with the rigid cartridge. A rigid wall includes a planar upper surface including an aperture. The aperture is configured to receive and fixedly engage a corresponding connector.

Some systems, devices and methods detailed herein provide a restraint device for holding a microslide or another slide instrument in an operative position in a petri dish.

Disclosed herein are compositions and methods for biological upgrading of electrocatalysis-derived formate presents a promising approach to sequester CO.sub.2, valorize curtailed electrons, and establish a sustainable formate bioeconomy.

The present disclosure relates to a process for electrostatic spray drying of a living microorganism, the process comprising the following steps: a. Providing a suspension, comprising a number of components, including a microorganism, a solvent and an additive; b. Applying an electrostatic charge to said suspension; c. Forming droplets of said suspension; d. Drying said droplets, thereby forming dried particles; and e. (Optionally) collecting the dried particles.