A device for DNA repair phototherapy is provided. A method of use for the device to provide DNA phototherapy to damaged DNA is also disclosed.

The present invention provides a cell treatment apparatus that can improve the operating rates of an observation unit and a laser irradiation unit in the case of treating a plurality of cell culture vessels. The cell treatment apparatus of the present invention includes: a cell treatment chamber in which cells in a cell culture vessel are treated; an observation unit that can observe the cells in the cell culture vessel; a laser irradiation unit that can irradiate the cells with lasers; a first moving unit that can move the observation unit; and a second moving unit that can move the laser irradiation unit, wherein the cell treatment chamber includes a plurality of regions in which the cells can be treated, each region includes a first cell culture vessel placement unit and a second cell culture vessel placement unit, in the first cell culture vessel placement unit, the cells in the cell culture vessel are observed by the observation unit, in the second cell culture vessel placement unit, the cells in the cell culture vessel are irradiated with lasers by the laser irradiation unit, the observation unit is moved by the first moving unit in a state of being able to observe the cells in the cell culture vessel in the first cell culture vessel placement unit of each region, and the laser irradiation unit is moved by the second moving unit in a state of being able to irradiate the cells in the cell culture vessel in the second cell culture vessel placement unit of each region with lasers.

A particle analysis device includes multiple stacked plates joined together; an upper liquid space adapted to store a first liquid; a lower liquid space adapted to store a second liquid; a connection pore connecting the upper liquid space to the lower liquid space; a first hole extending from the top surface to the upper liquid space, the first liquid flowing through the first hole; and a second hole extending from the top surface to the lower liquid space, the second liquid flowing through the second hole. A first electrode and a second electrode that are sheets are pinched between two of the plates. The first electrode applies an electric potential to the first liquid in the upper liquid space through the first hole, whereas the second electrode applies an electric potential to the second liquid in the lower liquid space through the second hole. The particle analysis device further includes a first electrode-rod-insertion hole extending from the top surface to the first electrode, and a second electrode-rod-insertion hole extending from the top surface to the second electrode. The first electrode and the second electrode are not exposed at any side surface of the particle analysis device.

A microfluidic system for causing perturbations in a cell membrane, the system including a microfluidic channel defining a lumen and being configured such that a cell suspended in a buffer can pass therethrough, wherein the microfluidic channel includes a cell-deforming constriction, wherein a diameter of the constriction is a function of the diameter of the cell.

A method of alignment and fusion of cells with an electrical field includes firing a first fluid dispenser of an electrofusion device until a first impedance sensor in a first microfluidic channel of the electrofusion device detects a presence of a first cell. The method includes firing a second fluid dispenser of the electrofusion device until a second impedance sensor in a second microfluidic channel of the electrofusion device detects a presence of a second cell. The method includes moving the first cell and the second cell into a merging chamber of the electrofusion device by firing a third fluid dispenser of the electrofusion device, in response to alignment of the first cell in the first microfluidic channel with the second cell in the second microfluidic channel. The method further includes fusing the first cell and the second cell in the merging chamber by creating an electrical field in the merging chamber.

A method to enhance homology directed recombination (HDR) efficiency, and a kit therefor, are provided.

Systems and methods for automated optical tweezer (OT)-based mitochondrial transfer are provided. The system for automated optical tweezer-based mitochondrial transfer includes a microfluidic device and an optical tweezer micromanipulation system. The microfluidic device includes one or more confinement means for confining cells and a channel for flowing mitochondria near the confinement means. The optical tweezer micromanipulation system is configured to trap at least one of the mitochondria within the channel of the microfluidic device for transport of the mitochondria to one of the confined cells.

The present invention relates to a flatbed air-liquid interface exposure module for exposing a plurality of cells at an air-liquid interface to nanoparticles, the flatbed air-liquid interface exposure module comprising a moisturizing section, an exposure section, and an aerosol duct. Further provided are related systems and methods.

Provided herein is a pipette tip for electroporation including an outer surface, a void located within the outer surface, and a conductor located at least partially on or within the outer surface, in electrical communication with at least a portion of the outer surface and the void. Further provided herein is a pipette tip for electroporation including a body, a connector, and an elongated part. The connector is located at the distal end of the body and further includes at least one connecting post, a connecting part in mechanical communication with the connector, and a conductor located at least partially on or within the connector, wherein the conductor surrounds at least a portion of the connecting post. The elongated part also has a void and is located at the distal end of the connector. The void of the body, connector, and elongated part are in fluid communication.

The present invention relates to a device (1) for treating cells for stimulating cell growth, comprising a unit (2) for generating and emitting electric pulses and an electric field resulting therefrom, and a treatment space (3) that can be penetrated by the emitted electric pulses, wherein the device (1) further comprises a unit (4, 9) which can be moved into and out of said treatment space (3) such that cell material provided within a compartment (4a) of said unit (4), or provided in a container (5) on or in said unit (4, 9), can be moved into and out of said treatment space (3), and wherein said treatment space (3) is provided between two electrodes (2a, 2b) or plates of a capacitor parallel to each other.