According to the present disclosure, there is provided a cell stimulation and culture platform using a ultrasonic hologram, including a culture vessel in which a culture well with an open lower portion is formed; a transmission sheet which is installed to cover a lower surface of the culture vessel and where a biological sample is seated; a platform body that is filled with a liquid medium and an open upper end is covered by the transmission sheet; an ultrasonic transducer that is installed inside the platform body in a state of being spaced apart from the biological sample; and an ultrasonic hologram lens that is installed on the ultrasonic transducer, and spatially modulates the phase of the ultrasonic waves using a surface structure designed to have different height distributions to focus the ultrasonic waves in a set pattern shape on a target surface on which the biological sample is located.

The present disclosure relates to a pneumatic microfluidic platform for high-throughput studies of cardiac hypertrophy that enables repetitive (hundreds of thousands of times) and robust (over several weeks) manipulation of cardiac μtissues. The platform is reusable for stable and reproducible mechanical stimulation of cardiac μtissues (each containing only 500 cells). Heterotypic and homotypic μtissues produced in the device were pneumatically loaded in a range of regimes, with real-time on-chip analysis of tissue phenotypes. Concentrated loading of the three-dimensional cardiac tissue faithfully recapitulated the pathology of volume overload seen in native heart tissue. Sustained volume overload of μtissues was sufficient to induce pathological cardiac remodeling associated with upregulation of the fetal gene program, in a dose-dependent manner.

The present invention delivers a substance such as a reagent into a cell. A tubular body coated with an electroconductive polymer for introducing a substance into a cell and/or recovering a substance from a cell. The use of a tubular body coated with an electroconductive polymer to introduce a substance into a cell and/or recover a substance from a cell. A device for introducing a substance into a cell and/or recovering a substance from a cell, the device being equipped with a substrate and a tubular body coated with an electroconductive polymer, and the tubular body being positioned on the substrate. A system for introducing a substance into a cell and/or recovering a substance from a cell, the system being equipped with the abovementioned device, a voltage supply unit for supplying a voltage, and, as needed, electrodes.

Embodiments described herein relate generally to a three-dimensional ex vivo skeletal muscle tissue comprising a hydrogel and a plurality of cells that includes skeletal muscle cells, at least a portion of the cells being encapsulated inside the hydrogel. In some embodiments, the skeletal muscle tissue is characterized by one or more contractions in response to an electrical and/or chemical stimulation.

Disclosed is an in vitro exposure system that may radiate a uniform field having a constant wavefront to an experimental cell container and expose each cell container to a same electromagnetic field.

Microfluidic device comprising at least one cell culture unit for forming, culturing, growing and/or maintaining a 3D tissue structure such as a 3D strip of cardiac tissue, wherein the at least one cell culture unit comprises: a respective culture chamber for culturing cells having a chamber outlet opening; and a cell supply channel arranged to guide a microfluidic flow of liquid holding cells between a channel inlet and a channel outlet, wherein the cell supply channel is provided with a flow inhibitor which is operable to selectively provide a flow inhibiting state or a flow permitting state depending on a fluid pressure at the flow inhibitor, wherein, in the flow inhibiting state, the flow inhibitor is configured to substantially inhibit liquid flow between the cell supply channel and the culture chamber, wherein, in the flow permitting state, the flow inhibitor is configured to permit such liquid flow such that the cell supply channel is in liquid communication with the culture chamber to supply the culture chamber with cells, wherein the culture chamber is provided with at least two mutually spaced apart elastic support structures which extend in the culture chamber and which are configured for elastically supporting a tissue formed in the culture chamber, in particular a cultured 3D tissue formed from the cells, wherein the elastic support structures are elastically deformable, in particular flexible, in particular to vary a mutual distance of said support structures under influence of a varying contraction force between said support structures.

The present disclosure is directed to devices, systems and methods that include a stage and an actuator configured to transmit a orthogonal force to the stage, wherein the actuator is configured to receive a plurality of orthogonal acceleration signals, wherein the orthogonal acceleration signals comprise an actuator frequency signal and an actuator magnitude signal.

A system and process for enhancing sensory attributes of a beverage product are described. According to one embodiment, the process comprises providing a consumable beverage. A sonication process is applied to the consumable beverage utilizing a sonication unit. Chemical reactions in the consumable beverage are catalyzed to modify a sensory attribute of the consumable beverage.

There is provided a bioreactor which is provided with a lid (13) that facilitates access to the incubation cavity. Specifically the end wall of the incubation cavity is constituted by the lid (13) so that removal of the cap renders the incubation cavity fully accessible.

This disclosure relates to methods of growing cells within a hydrogel scaffold and pressurizing the hydrogel and cells to induce the cells to stretch and differentiation. The disclosed method can include coating a substrate of a bioreactor with a hydrogel and seeding cells onto the hydrogel and/or the substrate. The disclosed method can further include growing the seeded cells into a cell mass and pressurizing the cell mass and the hydrogel within the bioreactor. Pressurizing the cell mass and the hydrogel induces the cell mass and hydrogel to mechanically stretch, thereby inducing hypertrophy and cell alignment.