The present disclosure relates to a cell processing container for use in one or more unit operations in cell and/or gene therapy manufacture, the container having a base section, a top section arranged substantially in parallel with the base section and a wall element arranged between the top section and the base section and defining an internal lumen of the container, in which the wall element of the cell culture container preferably is compressible with respect to the top and base section and the wall element of the cell culture container is composed of a flexible material, wherein the cell processing container comprises at least one sterile connector end configured to operatively couple with a further sterile connector end to form a sterile connector between the cell processing container and a further container to which the cell processing container is to be fluidly connected.

A cell processing device for use in performing one or more unit processes in one or more of cell or gene therapy manufacturing, comprising a cell processing platform fluidly coupled to at least one auxiliary container and to at least one primary container, the cell processing platform comprising a body portion comprising at least one fluid inlet fluidly connected to a fluid outlet, and an auxiliary container port fluidly coupled to the at least one fluid inlet of the body portion, wherein the at least one auxiliary container is received in sealing engagement with the auxiliary container port such that the auxiliary container lumen is fluidly connected with the at least one fluid inlet of the body portion, and a primary container is received in sealingly engagement with the primary container port such that the primary container lumen is fluidly connected with the fluid outlet of the body portion.

The present disclosure relates to a cell processing platform for use in one or more unit operations in cell and/or gene therapy manufacture, the platform comprising a body portion comprising at least one fluid inlet fluidly connected to a fluid outlet, and an auxiliary container port fluidly coupled to the at least one fluid inlet of the body portion, wherein the auxiliary container port is configured and arranged to receive and sealingly engage with an auxiliary container and to fluidly connect the auxiliary container lumen with the at least one fluid inlet of the body portion, and a primary container port configured and arranged to sealingly engage with a primary container and to fluidly connect the primary container lumen with the fluid outlet of the body portion.

Microfluidic devices are described that include a microfluidic channel, a first array of one or more magnets above the microfluidic channel, each magnet in the first array having a magnetic pole orientation opposite to a magnetic pole orientation of an adjacent magnet in the first array, and a second array of one or more magnets beneath the microfluidic channel, each magnet in the second array having a magnetic pole orientation opposite to a magnetic pole orientation of an adjacent magnet in the second array. The first array is aligned with respect to the second array such that magnetic fields emitted by the first array and second array generate a magnetic flux gradient profile extending through the channel. An absolute value of the profile includes a first maximum and a second maximum that bound a local minimum. The local minimum is located within the microfluidic channel or less than 5 mm away from a wall of the microfluidic channel. Methods of using the new devices are also described.

A compact device for measuring incubation of living cells with molecules attached to superparamagnetic nanoparticles consisting of iron cores by magnetizing the nanoparticles with an external pulsed field, measuring the time dependence of decaying magnetic fields of the nanoparticles as they attach to the cells by a magnetic sensor, extracting number of attached nanoparticles per cell and rate of incubation by mathematical analysis of the magnetic field emitted by the incubating cells versus time.

The present disclosure is generally directed to methods and devices for the precise and simultaneous optical irradiation and oscillating magnetic field radiation of a target, such as mammalian cells and/or nanostructures.

Assemblies, systems, and methods for isolation of target material are provided. In various embodiments, an assembly for target material isolation includes a housing having an upper portion and a lower portion together defining an inner chamber. The inner chamber includes a semi-toroidal shape and the semi-toroidal shape defines a longitudinal axis. The assembly further includes one or more fluidic connection from the exterior of the housing to the inner chamber. An isolation material (e.g., polymer wool and/or magnetic beads) may be disposed within the inner chamber. A system includes a configured to fit at least a portion of the housing and releasably couple the assembly. Upon activation of the motor, the assembly may rotate about the longitudinal axis. An angle of the platform may be adjustable to thereby change the angle of the longitudinal axis about which the assembly rotates.

Methods and devices are provided for simultaneous irradiation of a target, such as mammalian cells and/or nanostructures. The target is simultaneously exposed to optical irradiation and oscillating magnetic field radiation. The simultaneous optical irradiation and oscillating magnetic field radiation occur within an incubator-actuator device. The incubator-actuator device includes a sample chamber, a magnetic field generating coil, and, a light-emitting diode (LED) placement cage.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.