This invention is related to technology of tissue-engineered constructs biofabrication from tissue spheroids. This novel technology of scaffold-free, nozzle-free and label-free bioassembly opens a unique opportunity for rapid biofabrication of 3D tissue and organ constructs with complex geometry. A combination of intense magnetic and acoustic fields could enable rapid levitational bioassembly of complex-shaped 3D tissue constructs from tissue spheroids at low concentration of paramagnetic agent (gadolinium salt) in the medium. Magnetic field provides objects levitation due to field configuration with the lowest magnetic field density in the center of working volume of medium with tissue spheroids, and three-dimensional acoustic field forms internal and external construct geometry by means of acoustic radiation forces.

Devices for magnetic 3d culture are described including magnetic lids/bases for single Petri plates and adjustable height cap for same. Similar devices for multi-magnet culture plates wherein multiwell plates have all adjacent magnets orientated in the opposite polarity, and methods of making same.

The present invention provides an apparatus for applying electrical stimulation to cells in a liquid culture medium without using electrodes immersed in the liquid culture medium. This apparatus is an electrical stimulation apparatus A for electrically stimulating cells in a liquid culture medium (2). A liquid culture medium vessel (3) includes: a ring-shaped recess (7) for holding the liquid culture medium (2); and a through hole (6) formed within the ring-shaped recess (7). A magnetic core (1) is made of a magnetic material and disposed such that a portion thereof is inserted through the through hole (6) of the liquid culture medium vessel (3). An excitation coil (5) is wound around the magnetic core (1). A coil power supply (8) supplies a varying current to the excitation coil (5).

A method of producing a cell structure includes applying a magnetic field, in a container (40), to a plurality of culture carriers to arrange the culture carriers. The culture carriers include at least one of a carrier (10) and a cell holding carrier (30), the carrier (10) having a magnetic portion (12), formed only in a part of the carrier (10), and a cell holder (11). The method also includes culturing cells (20) held on the cell holder (11) while maintaining the culture carriers in the arranged state.

Assemblies, systems, and methods for isolation of target material are provided. In various embodiments, an assembly for target material isolation includes a housing having an upper portion and a lower potion together defining an inner chamber. The inner chamber includes a semi-toroidal shape and the semi-toroidal shape defines a longitudinal axis. The assembly further includes one or more fluidic connection from the exterior of the housing to the inner chamber. An isolation material (e.g., polymer wool and/or magnetic beads) may be disposed within the inner chamber. A system includes a configured to fit at least a portion of the housing and releasably couple the assembly. Upon activation of the motor, the assembly may rotate about the longitudinal axis. An angle of the platform may be adjustable to thereby change the angle of the longitudinal axis about which the assembly rotates.

Cell-culture platforms are described that can impart cyclic strain on a biologically-relevant culture substrate. Accordingly, systems and methods of the invention can provide a more accurate model of physiological strain and forces than earlier technologies. Systems of the invention enable high-throughput experimentation in standard culturing equipment, while utilizing a 3D physiologically-relevant environment.

A bioprocessing method for cell therapy includes the steps of genetically modifying a population of cells in a bioreactor vessel to produce a population of genetically modified cells, and expanding the population of genetically modified cells within the bioreactor vessel to generate a number of genetically modified cells sufficient for one or more doses for use in a cell therapy treatment without removing the population of genetically modified cells from the bioreactor vessel. Cells are settled on a gas permeable, liquid impermeable membrane for expansion, and are resuspended when, or after, the desired cell density is reached.

Systems and methods generally useful in medicine, cellular biology, nanotechnology, and cell culturing are discussed. In particular, at least in some embodiments, systems and methods for magnetic guidance and patterning of cells and materials are discussed. Some specific applications of these systems and methods may include levitated culturing of cells away from a surface, making and manipulating patterns of levitated cells, and patterning culturing of cells on a surface. Specifically, a method of culturing cells is presented. The method may comprise providing a plurality of cells, providing a magnetic field, and levitating at least some of the plurality of cells in the magnetic field, wherein the plurality of cells comprise magnetic nanoparticles. The method may also comprise maintaining the levitation for a time sufficient to permit cell growth to form an assembly.

The present invention provides a cell culture base that avoids contact between a photoresponsive layer and cells. The cell culture base of the present invention includes: a support layer; a photoresponsive layer; and a cell culture layer, wherein the photoresponsive layer is laminated on the support layer, the cell culture layer is laminated on the photoresponsive layer, the photoresponsive layer contains a photoresponsive substance that causes at least one of heat and change in magnetism by light irradiation, and the cell culture layer is capable of culturing cells.

This present invention provides means for cell harvesting and isolation to be carried out with a single apparatus. This represents a more convenient way for the processing of cells compared with known methods and represents a useful development for the manufacture of cell therapies.